Apoptotic Changes in Gingiva Caused by Smoking
Märksõnad
Abstraktne
Kirjeldus
Periodontal disease disrupts soft tissue metabolism in the gingiva through a decrease in the production of collagen, the quality, and quantity of the connective tissue. The etiology and pathogenesis of chronic periodontitis are mostly revealed, however, the mechanism of environmental factors such as smoking yet to be clarified. Major consequences of smoking in gingival tissues are suggested to be the reduction in neutrophil and fibroblast function, decreased immunoglobulin G production, increased periodontal pathogen bacteria prevalence, difficulty in eliminating pathogens with mechanical therapy, and reduction in growth factor production. In the present study, markers of tissue destruction, matrix metalloproteinase-8 and tissue inhibitor of matrix metalloproteinase-1, hypoxia markers, vascular endothelial growth factor and hypoxia-inducible factor and apoptotic markers, bax, bcl-2, and caspase-3 were evaluated.
Kuupäevad
| Viimati kinnitatud: | 07/31/2018 |
| Esmalt esitatud: | 08/12/2018 |
| Hinnanguline registreerumine on esitatud: | 08/12/2018 |
| Esmalt postitatud: | 08/14/2018 |
| Viimane värskendus on esitatud: | 08/14/2018 |
| Viimati värskendus postitatud: | 08/15/2018 |
| Õppe tegelik alguskuupäev: | 01/09/2017 |
| Eeldatav esmane lõpetamise kuupäev: | 01/09/2018 |
| Eeldatav uuringu lõpetamise kuupäev: | 04/09/2018 |
Seisund või haigus
Faas
Käerühmad
| Arm | Sekkumine / ravi |
|---|---|
| Healthy individuals Gingival biopsies of healthy individuals who were never-smokers and had no systemic or oral disease or condition. | |
| periodontitis patients Gingival biopsies of periodontitis patients who were never-smokers and had no systemic disease or condition. | |
| Healthy smokers Gingival biopsies of healthy individuals who were smokers but no systemic or oral disease or condition. | |
| Smokers with periodontitis Gingival biopsies of periodontitis patients who were smokers but no systemic disease or condition. |
Abikõlblikkuse kriteeriumid
| Õppimiseks sobivad vanused | 30 Years To 30 Years |
| Uuringuks kõlblikud sood | All |
| Proovivõtumeetod | Probability Sample |
| Võtab vastu tervislikke vabatahtlikke | Jah |
| Kriteeriumid | Inclusion Criteria: - Age range from 30 to 45, - the existence of at least 20 functioning teeth, - systemical health, - no antibiotic use within 6 months, - no periodontal therapy within 6 months, - no pregnancy or lactation, - no drug use, in addition; for the smokers existence of the smoking condition for at least five years. Exclusion Criteria: - Patients younger than 30 older than 50 years old, - the absence of occlusion, - drug use, - pregnancy/lactation, - previous antibiotic use, - previous periodontal therapy, - the existence of any systemical disease. |
Tulemus
Esmased tulemusnäitajad
1. Fibroblast and total inflammatory cell counts [Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after.]
Sekundaarsed tulemusmõõdud
1. Apoptotic markers [Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after]
2. Hypoxia and tissue destruction markers [Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after]
