NAC Supplementation and Skeletal Muscle Performance
Märksõnad
Abstraktne
Kirjeldus
The major thiol-disulfide couple of reduced (GSH) and oxidized glutathione (GSSG) is a key-regulator of major transcriptional pathways regulating aseptic inflammation and recovery of skeletal muscle following aseptic injury. Antioxidant supplementation may hamper exercise-induced cellular adaptations.
Our objective was to examine how thiol-based antioxidant supplementation affects skeletal muscle's performance and redox-sensitive signalling during the inflammatory and repair phases associated with exercise-induced micro-trauma.In a double-blind, counterbalanced design, 12 men received placebo (PLA) or N-acetylcysteine (NAC, 20 mg/kg/day) following muscle-damaging exercise (300 eccentric contractions). In each trial, muscle performance was measured at baseline, post-exercise, 2h post-exercise and daily for 8 consecutive days. Muscle biopsies from vastus lateralis and blood samples were collected pre-exercise and 2h, 2d, and 8d post-exercise.
Kuupäevad
| Viimati kinnitatud: | 12/31/2012 |
| Esmalt esitatud: | 01/20/2013 |
| Hinnanguline registreerumine on esitatud: | 01/24/2013 |
| Esmalt postitatud: | 01/28/2013 |
| Viimane värskendus on esitatud: | 01/24/2013 |
| Viimati värskendus postitatud: | 01/28/2013 |
| Õppe tegelik alguskuupäev: | 12/31/2009 |
| Eeldatav esmane lõpetamise kuupäev: | 08/31/2011 |
| Eeldatav uuringu lõpetamise kuupäev: | 03/31/2012 |
Seisund või haigus
Sekkumine / ravi
Dietary Supplement: n-acetylcysteine/placebo supplementation
Faas
Käerühmad
| Arm | Sekkumine / ravi |
|---|---|
| Experimental: n-acetylcysteine/placebo supplementation n-acetylcysteine supplementation, orally in three daily dosages, at 20 mg/kg/day, daily for eight days after exercise placebo, orally in three daily dosages, content: 500 mL drink that contained water (375 mL), sugar-free cordial (125 ml), and 2 g of low-calorie glucose/dextrose powder. | Dietary Supplement: n-acetylcysteine/placebo supplementation n-acetylcysteine administration: 20 mg//kg/day, orally, daily for eight days following exercise
placebo administration: 500 mL orally, daily for eight days following exercise |
Abikõlblikkuse kriteeriumid
| Õppimiseks sobivad vanused | 18 Years To 18 Years |
| Uuringuks kõlblikud sood | Male |
| Võtab vastu tervislikke vabatahtlikke | Jah |
| Kriteeriumid | Inclusion Criteria: a) recreationally trained as evidenced by their maximal oxygen consumption levels (VO2max >45 ml/kg/min), b) were engaged in systematic exercise at least three times/week for ≥12 months), c) non-smokers, d) abstained from any vigorous physical activity during the study, e)abstained from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, and medications during the study. Exclusion Criteria: a) a known NAC intolerance or allergy, b) a recent febrile illness, c) history of muscle lesion, d) lower limb trauma |
Tulemus
Esmased tulemusnäitajad
1. Change in reduced glutathione in blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
2. Change in reduced glutathione in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
3. Change in protein carbonyls in red blood cells and serum [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
4. Change in protein carbonyls in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
5. Change in thiobarbituric acid reactive substances in red blood cells and serum [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
6. Change in thiobarbituric acid reactive substances in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
7. Change in oxidized glutathione in red blood cells and blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
8. Change in total antioxidant capacity in serum [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
9. Change in oxidized glutathione in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
10. Change in catalase activity in red blood cells and serum [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
11. Change in glutathione peroxidase activity in red blood cells [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
12. Change in creatine kinase activity in plasma [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
13. Change in C-reactive protein in plasma [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
14. Change in macrophage infiltration in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
15. Change in white blood cell count in blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
16. Change in neutrophil count in blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
17. Change in fatty acid binding protein in plasma [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
18. Change in cortisol concentration in blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
19. Change in testosterone concentration in plasma [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
20. Change in cytokine concentration in plasma [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
21. Change in adhesion molecule concentration in blood [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
22. Change in intracellular signalling proteins in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
23. Change in myogenic determination factor (MyoD) protein levels in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
24. Change in tumor necrosis factor α in muscle [one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise]
Sekundaarsed tulemusmõõdud
1. Change in muscle function of knee extensor and flexor muscle [one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise]
2. Body composition [One day before exercise]
3. Maximal aerobic capacity [One day before exercise]
4. Change in profile of dietary intake [one hour before exercise, daily for 8 days post-exercise]
5. Change in side effect occurence [one hour before exercise, daily for 8 days post-exercise]
