Oxidative Stress

Endothelial Function

Cardiovascular System

Hypertension

Lactose placebo for pills and saline for perfusion.

Placebos

Febuxostat

Rasburicase

Erasme Hospital

Uric Acid Effects on Endothelium and Oxydative Stress

Differential Effects of Uric Acid and Xanthine Oxidoreductase on Endothelial Function and Oxydative Stress

Perfusion unit (Laser Doppler Imager + iontophoresis of (ACh and SNP and hyperemia with ou without L-NAME). Assessment of endothelial function.

Carotido-femoral and carotido-radial pulse wave velocity and pulse wave analysis

Beat-to-beat measurements with Finapres and manually

eNOS, NOX, XO we will incubate endothelial cells with plasma or serum from subjects. Then we will measure the sus-mentionned enzymes' expression.

We will perform proteomics or metabolomics analysis directly on serum from participants and also on lysate of endothelial cells pre-incubated with plasma or serum from participants.

Benjamin De Becker

Investigator, Clinical Research

Erasme University Hospital

Fonds Erasme

Fonds National de la Recherche Scientifique

This is a prospective, randomized, placebo-controlled, double-blinded and 3 ways cross-over study.
Three populations : healthy male subjects under 40 y [1] ; healthy male and female participants between 40 and 65 years [2] and subjects with hypertension [3]. Minimum 15 participants in each group are needed.
The populations 2 and 3 will be studied together with subgroups analyses of the results for the status of hypertension, of treatment, age and gender.
For 5 subjects in each group, we will perform a fourth session with Placebo PO and Rasburicase IV. That session will be non-randomized and blinded only for the subject.

Febuxostat and Rasburicase will be prepared by an independant pharmacist.

l arginine/palavik

Uric Acid Effects on Endothelium and Oxydative Stress

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