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rubella/glutatioon
Link salvestatakse lõikelauale
9 tulemused
Genome-Wide Analysis of the Glutathione S-Transferase Gene Family in Capsella rubella: Identification, Expression, and Biochemical Functions.
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Logi sisse
Extensive subfunctionalization might explain why so many genes have been maintained after gene duplication, which provides the engine for gene family expansion. However, it is still a particular challenge to trace the evolutionary dynamics and features of functional divergences in a supergene family
Recombinant rubella E1 fusion proteins for antibody screening and diagnosis.
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BACKGROUND
Until rubella is eradicated there will be a continuing need for rubella antibody surveillance. Antigen production using recombinant DNA technology may be a viable alternative to traditional techniques of producing antigens for enzyme immunoassays (EIAs).
OBJECTIVE
To investigate the
Identification of an RNA-stimulated NTPase in the predicted helicase sequence of the Rubella virus nonstructural polyprotein.
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Logi sisse
The replicative proteins of Rubella virus are generated from a polyprotein that is translated from the 5'-terminal segment of the viral genome. The determination of the genome sequence and the description of amino acid sequence motifs which are proposed to be characteristic for helicase proteins
Quantification of neomycin in rubella vaccine by off/on metal ion mediated photoluminescence from functionalized graphene quantum dots.
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Logi sisse
The determination of neomycin sulfate was made using photoluminescent amino-functionalized graphene quantum dots (obtained from hydro-exfoliation of a mixture of citric acid and glutathione). From the several ions tested, Fe3+ was the best mediator to enable an off/on photoluminescence
Validation of monoplex assays detecting antibodies against Corynebacterium diphtheriae and Clostridium tetani toxins, rubella virus and parvovirus B19 for incorporation into Multiplex Serology.
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Logi sisse
Serological assays detecting antibodies in serum or plasma samples are useful and versatile instruments to investigate an individual's infection and vaccination history, e.g. for clinical diagnosis, personal risk evaluation, and seroepidemiological studies. Multiplex Serology is a suspension bead
Use of rubella virus E1 fusion proteins for detection of rubella virus antibodies.
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Logi sisse
Two glutathione S-transferase fusion proteins containing 44 (p1503) and 75 (p1509) amino acid residues of the rubella virus E1 glycoprotein were expressed in Escherichia coli with the aim of producing a recombinant rubella virus antigen for use in serological assays. p1503 contained three
[The Preparation of Epitope-based Recombinant Rubella Virus Diagnostic Antigen].
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Logi sisse
To prepare an epitope-based recombinant Rubella virus (RV) recombinant diagnostic antigen(designated ‘H29’) and preliminarily evaluate its antigenicity. With Glutathione S-Transferase (GST) located at the N-terminal, and the His tag at the C-terminal, the epitope-based RV recombinant diagnostic
Binding of cellular p32 protein to the rubella virus P150 replicase protein via PxxPxR motifs.
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Logi sisse
A proline-rich region (PRR) within the rubella virus (RUBV) P150 replicase protein that contains three SH3 domain-binding motifs (PxxPxR) was investigated for its ability to bind cell proteins. Pull-down experiments using a glutathione S-transferase-PRR fusion revealed PxxPxR motif-specific binding
Avoidance of oxidative-stress perturbation in yeast bioprocesses by proteomic and genomic biostrategies?
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OBJECTIVE
Bioprocess oxidative stress caused by many reactive oxygen species (ROS) can lead to largely irreversible perturbation of yeast bioprocesses. These include the production of proteins derived from recombinant DNA yeast technology (aerobically grown Saccharomyces cerevisiae). These proteins
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