[Study on the alkaloids in Stephania hernandifolia].

OBJECTIVE

To study the alkaloids in Stephania hernandifolia.

METHODS

The dried herbs of S. hernandifolia were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatography on silica gel, Rp-18, MCI CHP 20P, Sephadex LH-20 were applied for the isolation and purification of the crude alkaloids fraction. The structures were elucidated by their physicochemical properties and spectral data.

RESULTS

Six alkaloids were obtained and identified as Cepharamine (I), l-tetrahadropalmatine (II), Plmatine (III), Stephamiersine (IV), Telitoxine (V), Daurioxoisoporphine D (VI).

CONCLUSIONS

Compounds I - VI are isolated from this plant for the first time, and compounds V, VI are isolated from the plants of Stephania for the first time.