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An extractive removal step optimized for a high-throughput α-cellulose extraction method for δ13C and δ18O stable isotope ratio analysis in conifer tree rings.

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Stable isotope ratios (δ13C and δ18O) of tree-ring α-cellulose are important tools in paleoclimatology, ecology, plant physiology and genetics. The Multiple Sample Isolation System for Solids (MSISS) was a major advance in the tree-ring α-cellulose extraction methods, offering greater throughput and

The effects of α-cellulose extraction and blue-stain fungus on retrospective studies of carbon and oxygen isotope variation in live and dead trees.

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Tree-ring carbon and oxygen isotope ratios from live and recently dead trees may reveal important mechanisms of tree mortality. However, wood decay in dead trees may alter the δ(13)C and δ(18)O values of whole wood obscuring the isotopic signal associated with factors leading up to and including

δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.

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Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate. Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted

Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases.

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Loblolly pine residues have enormous potential to be the raw material for advanced biofuel production due to extensive sources and high cellulose content. Hot water (HW) pretreatment, while being a relatively economical and clean technology for the deconstruction of lignocellulosic biomass, could

Identification of QTLs influencing wood property traits in loblolly pine ( Pinus taeda L.). II. Chemical wood properties.

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Chemical wood property traits were analyzed for the presence of quantitative trait loci (QTLs) in a three-generation outbred pedigree of loblolly pine ( Pinus taeda L.). These traits were assayed using pyrolysis molecular beam mass spectrometry and include mass spectrum peak intensities associated

Comparison of cellulose extraction methods for analysis of stable-isotope ratios of carbon and oxygen in plant material.

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The Jayme-Wise and diglyme-HCl methods for extracting cellulose from plant material for stable-isotope analysis differ considerably in ease of use, with the latter requiring significantly less time and specialized equipment. However, the diglyme-HCl method leaves a small lignin residue in the crude

Comparison of δ(13)C and δ(18)O from cellulose, whole wood, and resin-free whole wood from an old high elevation Pinus uncinata in the Spanish central Pyrenees.

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δ(13)C and δ(18)O values from sapwood of a single Pinus uncinata tree, from a high elevation site in the Spanish Pyrenees, were determined to evaluate the differences between whole wood and resin-free whole wood. This issue is addressed for the first time with P. uncinata over a 38-year long period.

A rapid and simple method for processing wood to crude cellulose for analysis of stable carbon isotopes in tree rings.

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For analysis of carbon isotope discrimination in wood, cellulose or holocellulose is often preferred to whole tissue because of the variability in isotopic composition of different wood components and the relative immobility of cellulose. Most currently used methods for the preparation of wood

Microanalytical method for the characterization of fiber components and morphology of woody plants.

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Microanalytical techniques were developed which allow the rapid characterization of fiber components and morphology of loblolly pine in a large number of samples. These techniques consist of extractives removal, holocellulose preparation, alpha-cellulose and lignin content determination, and fiber

Nucleotide variation in genes involved in wood formation in two pine species.

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Nucleotide diversity in eight genes related to wood formation was investigated in two pine species, Pinus pinaster and P. radiata. The nucleotide diversity patterns observed and their properties were compared between the two species according to the specific characteristics of the samples analysed.

Transcriptional and computational study of expansins differentially expressed in response to inclination in radiata pine.

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Plants have the ability to reorient their vertical growth when exposed to inclination. This response can be as quick as 2 h in inclined young pine (Pinus radiata D. Don) seedlings, with over accumulation of lignin observed after 9 days s. Several studies have identified expansins involved in cell

Tree-ring isotope records of tropical cyclone activity.

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The destruction wrought by North Atlantic hurricanes in 2004 and 2005 dramatically emphasizes the need for better understanding of tropical cyclone activity apart from the records provided by meteorological data and historical documentation. We present a 220-year record of oxygen isotope values of

Stable carbon isotopes of glucose received from pine tree-rings as bioindicators of local industrial emission of CO2 in Niepołomice Forest (1950-2000).

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The mass spectrometric investigations of carbon isotope composition of glucose received from α-cellulose samples derived from Scots pine (Pinus sylvestris L.) growing in Niepołomice Forest were the main aim of this study. The annual rings covered the time span from 1950 to 2000. α-Cellulose samples

The Impact of Climate, Sulfur Dioxide, and Industrial Dust on δ18O and δ13C in Glucose from Pine Tree Rings Growing in an Industrialized Area in the Southern Part of Poland.

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The mass spectrometric analysis of the impact of sulfur dioxide and dust emission on carbon and oxygen stable isotopic compositions of glucose hydrolysed from α-cellulose samples extracted from Scots pine growing in the vicinity of "Huta Katowice" steelworks was the main aim of this study. The

Characterization of Wood-Plastic Composites Made with Different Lignocellulosic Materials that Vary in Their Morphology, Chemical Composition and Thermal Stability.

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In this study, four kinds of lignocellulosic fibers (LFs), namely, those from Chinese fir (Cunninghamia lanceolata), Taiwan red pine (Pinus taiwanensis), India-charcoal trema (Trema orientalis) and makino bamboo (Phyllostachys makinoi), were selected as reinforcements and incorporated into

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Herbal & Natural Medicine

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