Acute Undifferentiated Fever in Ethiopia
Avainsanat
Abstrakti
Kuvaus
General objective:
To assess the causes of acute undifferentiated febrile illness and evaluation of biomarkers for differentiation of bacterial and viral infections among outpatients at University of Gondar (UOG) Hospital, Northwest Ethiopia
Specific objectives
1. To determine the number of malaria cases, bacterial infections (by blood culture and polymearase chain reaction (PCR) for Rickettsia and Borrelia), and arboviral infections (DENV, YFV, CHIKV) among all acute febrile patients
2. To evaluate the diagnostic performance different assays (RDT, RT-(reverse transcriptase)PCR, ELISA) for the diagnosis of DENV
3. To evaluate the qualitative detection of C-reactive protein (CRP) and Myxovirus resistance protein (MxA) (by FebriDx RDT) and quantitative CRP detection for differentiating bacterial and viral infections
Study design, population, materials and methods: a cross-sectional cohort study on febrile patients presenting with acute fever at the emergency ward of the UOG hospital from June to August 2019. Clinical and epidemiological data will be recorded in a pseudo-anonymized and collected using an electronic data collection tool (KoBoToolbox). Blood will be collected for RDT testing, blood culture, PCR and serum for ELISA and RT-PCR.
Sample size: 200 acute febrile patients
Expected results and relevance: Evaluation of the causes of acute febrile illness and the role of biomarkers in differentiating viral and bacterial infections will increase the awareness of circulating pathogens and improve patient management. This evidence will contribute to a more rational use of laboratory diagnostic tests, antibiotics and antimalarial treatment.
Päivämäärät
| Viimeksi vahvistettu: | 01/31/2020 |
| Ensimmäinen lähetys: | 02/10/2020 |
| Arvioitu ilmoittautuminen lähetetty: | 02/10/2020 |
| Ensimmäinen lähetetty: | 02/12/2020 |
| Viimeisin päivitys lähetetty: | 02/13/2020 |
| Viimeisin päivitys lähetetty: | 02/17/2020 |
| Todellinen opintojen alkamispäivä: | 05/31/2019 |
| Arvioitu ensisijainen valmistumispäivä: | 08/30/2019 |
| Arvioitu tutkimuksen valmistumispäivä: | 12/29/2020 |
Ehto tai tauti
Vaihe
Kelpoisuusehdot
| Tutkimukseen soveltuvat iät | 15 Years Vastaanottaja 15 Years |
| Sukupuolet, jotka ovat kelpoisia tutkimukseen | All |
| Näytteenottomenetelmä | Probability Sample |
| Hyväksyy terveelliset vapaaehtoiset | Ei |
| Kriteeri | Inclusion Criteria: - Patients presenting with acute fever (an axillary temperature of ≥37.5°C; symptom duration ≤7 days) - ≥ 15 years old - signed informed consent Exclusion Criteria: - pregnant woman & children (<15 y) - febrile patient suspected of urinary tract infection or acute respiratory tract infection - Individuals who had taken antimicrobial and antimalarial drug in the last 2 weeks - Patients with acute injury or trauma or for which participation in the study implies an unacceptable health risk as determined by the physicians |
Tulokset
Ensisijaiset tulosmittaukset
1. The differentiation of bacterial infections and viral infections among acute febrile patients with biomarkers [within 7 days after onset of fever]
Toissijaiset tulosmittaukset
1. The number of malaria cases, bacterial infections, viral infections and unknown etiologies among acute febrile patients in Gondar [within 7 days after onset of fever]
2. The comparison of different assays for diagnosis of DENV [within 7 days after onset of fever]
3. The comparison of the qualitative and qualitative detection of CRP for differentiating bacterial and viral infections [within 7 days after onset of fever]
