Hepatitis C Virus Detection in Oral Squamous Cell Carcinoma
Avainsanat
Abstrakti
Kuvaus
Patient / Population: Paraffin embedded tissue samples of OSCC. Indicator: OSCC patients Control: Normal patients Formalin fixed paraffin embedded specimens from blocks retrieved from the archives of the Oral and Maxillofacial Pathology Department, Faculty of dentistry, Kasr Alini hospital setting from January 2014 till February 2018. All clinical and histopathological data from medical reports, will be reviewed and data will be collected regarding age, sex, tumor location, history of smoking and alcohol consumption, nodal status, tumor recurrences and histological classification of the patients. The specimens will be obtained from 2 groups:-
1. OSCC specimens. Histological diagnosis of H&E stained sections will be performed according to World health organisation (WHO) guidelines by 2 independent pathologists.
2. Normal oral mucosa specimens (control).
outcome: I) Immunohistochemical detection of Hepatitis C core antigen (Hep C cAg) : sc-58144, santa cruz biotechnology, USA) a mouse monoclonal antibody raised against Hepatitis C virus will be purchased and used for immunohistochemical staining. Staining procedure using an automated system Ventana Benchmark. The four µm thick paraffin embedded tissue sections will be deparaffinized in three changes of xylene then microwaved for antigen retrieval. The slides will be incubated with primary antibody overnight in the humidity chamber at room temperature. Then, the slides will be incubated with secondary antibody. The peroxidase activity will be made visible with diaminobenzidine. Finally, counterstaining will be obtained using Mayer's hematoxylin.
The immunostained sections will be examined using analyzer computer system (Germany).
Päivämäärät
| Viimeksi vahvistettu: | 01/31/2020 |
| Ensimmäinen lähetys: | 04/02/2017 |
| Arvioitu ilmoittautuminen lähetetty: | 04/05/2017 |
| Ensimmäinen lähetetty: | 04/11/2017 |
| Viimeisin päivitys lähetetty: | 01/31/2020 |
| Viimeisin päivitys lähetetty: | 02/04/2020 |
| Todellinen opintojen alkamispäivä: | 09/05/2017 |
| Arvioitu ensisijainen valmistumispäivä: | 02/06/2018 |
| Arvioitu tutkimuksen valmistumispäivä: | 09/14/2018 |
Ehto tai tauti
Interventio / hoito
Other: oral squamous cell carcinoma
Vaihe
Varren ryhmät
| Varsi | Interventio / hoito |
|---|---|
| Oral squamous cell carcinoma Sections (4-5 microns thick) will be cut for the immunohistochemical procedure, from each paraffin block and placed on positively charged (Opti-plus) slides by the technicians in the Oral and Maxillofacial laboratory setting.
Hep C cAg : sc-58144, santa cruz biotechnology, USA) a mouse monoclonal antibody raised against Hepatitis C virus will be purchased and used for immunohistochemical staining. | |
| Control Sections (4-5 microns thick) will be cut for the immunohistochemical procedure, from each paraffin block and placed on positively charged (Opti-plus) slides by the technicians in the Oral and Maxillofacial laboratory setting.
Hep C cAg : sc-58144, santa cruz biotechnology, USA) a mouse monoclonal antibody raised against Hepatitis C virus will be purchased and used for immunohistochemical staining. |
Kelpoisuusehdot
| Sukupuolet, jotka ovat kelpoisia tutkimukseen | All |
| Näytteenottomenetelmä | Probability Sample |
| Hyväksyy terveelliset vapaaehtoiset | Joo |
| Kriteeri | Inclusion Criteria: 1. Presence of sufficient paraffin-embedded tumor material; 2. Presence of oral cavity cancer (including oral tongue, floor of mouth, gingiva, lips, buccal mucosa) 3. Absence of recurrent head and neck cancer 4. Absence of previous oncologic therapy; 5. Histological-proven squamous cell carcinoma. - Exclusion Criteria: subjects with missing information on age or sex will be excluded. - |
Tulokset
Ensisijaiset tulosmittaukset
1. Hep C cAg by Immunohistochemistry [5 years]
