Neurodegenerative Diseases

The aim is to evaluate the role of ccf-mtDNA as a specific and early biomarker for different clinical pictures

cif mtDNA biomarker

Stefano Gambardella

Ccf mtDNA as a Neurodegenerative Biomarker

Ccf mtDNA as a Biomarker in Neurological and Neurodegenerative Diseases

Radiological and neuro and physiological approces; different laboratory test (CSF analysis,ematological test);neurological impairment is assessed with Expanded Disability Status Scale and through radiological assessment, cognitive impairment.

The plasma levels of GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL- 8, IL-10, and TNF-α will be measured using the Human Cytokine Magnetic 10-Plex Panel (Thermo Fisher Scientific) according to the manufacturer's instructions. We will add the same plasma sample to each plate for cytokine multiplex assay and calculated inter assay CV (%). The quantization of plasma cytokines will be compared with the quantization of the ccf mtDNA and the clinical stage of the pathology

Data will be presented as the mean ± standard deviation (SD). Categorical variables will compared using a chi-squared test. For a comparison of averages for the two groups, a Student's t-test will be used. When the data set will not normally distributed, a Mann-Whitney U-test will be used. Analysis of covariance (ANCOVA) controlling for age and sex will examined to evaluate the effects of age and sex on CFS, plasma or serum mtDNA copy number and cytokine levels. For a comparison of four groups, a one-way analysis of variance (ANOVA) followed by multiple comparisons with Tukey's method will used. Correlation coefficients between mtDNA copy number in plasma and cytokines will be calculated using Spearman's rank correlation coefficients. A P-value < 0.05 will considered as statistically significant and will corrected for multiple comparisons using Bonferroni's method.

Doctor, Principal Investigator

Neuromed IRCCS

acute ischemic stroke patients within 6h after stroke onset

Ischemic Stroke

There is no intervention at all.

Xuanwu Hospital

Molecular Mechanisms of microRNA-494 Involving in Cerebral Ischemia

Study on the Molecular Mechanism of miR-494 Mediating Cell Cycle Regulation Following Cerebral Ischemia

Individual participant data for all primary outcome measures will be made available.

Ji Xunming,MD,PhD

Vice President of Xuanwu Hospital,Capital Medical University

Capital Medical University

Adults only, all genders, US-born African American

Hypertension

National Institutes of Health Clinical Center, 9000 Rockville Pike

Genomics, Environmental Factors and Social Determinants of Cardiovascular Disease in African-Americans Study (GENE-FORECAST)

GENE-FORECAST(R): Genomics, Environmental Factors and Social Determinants of Cardiovascular Disease in African-Americans Study

Our objective is to develop a community-based cohort and novel genomic science resource for defining the biological significance of ancestry-related genomic variation in African-Americans within the GENE FORECAST (R): GENomics, Environmental FactORs and the Social DEterminants of Cardiovascular Disease in African Americans STudy.

histone/stroke

Ccf mtDNA as a Neurodegenerative Biomarker

Molecular Mechanisms of microRNA-494 Involving in Cerebral Ischemia

Genomics, Environmental Factors and Social Determinants of Cardiovascular Disease in African-Americans Study (GENE-FORECAST)

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