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mannitol/peruna

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Changes in carbohydrate content of potato calli during osmotic stress induced by mannitol.

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Endogenous carbohydrate (fructose, glucose and sucrose) fractions were measured in calli of potato genotypes with different field tolerance to drought. Under in vitro stress conditions induced by 0.8 M mannitol, sucrose level of calli increased extremely in medium-tolerant (by 424.5%) and sensitive

Penetration of Mannitol into Potato Discs.

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Growth and developmental responses of potato to osmotic stress under in vitro conditions.

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The effect of mannitol on different genotypes of potato was studied in callus and plantlet culture. In vitro responses of five potato genotypes with well-known field behaviour to water deficit were analysed. After a 4-week-long cultivation on media containing mannitol up to 0.8 M, different

Use of potato extract broth for culturing root-nodule bacteria.

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Liquid media containing potato extract and 1% of glucose or sucrose were used to culture root-nodule bacteria (rhizobia) in shaken Erlenmeyer flasks. For comparison, these bacteria were also cultured in yeast extract-mannitol broth (YEMB) as a standard medium. Proliferation of rhizobia was monitored

Relationship between the electrical and rheological properties of potato tuber tissue after various forms of processing.

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The impedance at frequencies of 1-1000 kHz and dynamic bending storage modulus measured by the vibrating reed method were compared for potato tuber tissue, which had been processed by various methods. Raw potato tuber tissue strips were either heated for 30 min up to 100 degrees C or frozen-thawed.

Tissue distribution and change in potato starch phosphorylase mRNA levels in wounded tissue and sprouting tubers.

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Starch phosphorylase has been cloned from a lambda gt10 cDNA library of potato tuber mRNA. Selected recombinants have been used to demonstrate that phosphorylase mRNA is most abundant in tubers but is also detectable in stolon, root, stem and leaf tissue. The level of phosphorylase mRNA was greatly
Experiments were performed using naturally sunlit Soil-Plant-Atmosphere Research chambers that provided ambient or twice ambient CO2. Potato plants were grown in pots that were water sufficient (W), water insufficient for 12-18 days during both vegetative and tuber development stages (VR), or water

Production and characterization of fengycin by indigenous Bacillus subtilis F29-3 originating from a potato farm.

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Fengycin, a lipopeptide biosurfactant, was produced by indigenous Bacillus subtilis F29-3 isolated from a potato farm. Although inhibiting the growth of filamentous fungi, the fengycin is ineffective against yeast and bacteria. In this study, fengycin was isolated from fermentation broth of B.

Ralstonia solanacearum Race 3, Biovar 2, the Causal Agent of Brown Rot of Potato, Identified in Geraniums in Pennsylvania, Delaware, and Connecticut.

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The Plant Disease Diagnostic Laboratory of the Pennsylvania Department of Agriculture received diseased geranium (Pelargonium × hortorum) samples from several Pennsylvania (PA) greenhouses in 1999 and 2000 and from one Delaware (DE) greenhouse in 1999. Originating from Guatemala, plants exhibited

Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts.

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A method for regenerating plants from petiole protoplasts of the in vitro-raised sweet potato cultivar Jewel is described. Protoplast yields of 3.0-5.0×106 were obtained following 4-6 h digestion of 1- to 2-cm petioles (1 g fresh weight) with 1% Cellulase-R10, 2% Macerozyme-R10, and 0.3%

Insecticidal activity of some reducing sugars against the sweet potato whitefly, Bemisia tabaci, Biotype B.

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The effects of 16 sugars (arabinose, cellobiose, fructose, galactose, gentiobiose, glucose, inositol, lactose, maltose, mannitol (a sugar alcohol), mannose, melibiose, ribose, sorbitol, trehalose, and xylose) on sweet potato whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) survival were

Wound-inducible potato inhibitor II genes: enhancement of expression by sucrose.

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Expression of a chimeric potato Inhibitor II-CAT gene in transgenic tobacco plants was enhanced 50-fold when leaf tissue was floated on solutions containing 1% sucrose. The expression of the chimeric gene was also enhanced when leaf sections were floated on solutions of glucose, fructose, and

Plant regeneration of mesophyll protoplasts from axenic potato (Solanum tuberosum L.) shoot.

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Mesophyll protoplasts from commercial potatoes were cultured in shallow liquid media. The regenerated cells divided and formed calli. Regenerant plants of two potato lines (Ke Xin 4; 68-62) were obtained from the regenerated calli after transfer to solid medium. The division frequency of the

Induction of alcohol dehydrogenase in explants of potato tuber (Solanum tuberosum L.).

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During callus formation a huge increase in alcoholdehydrogenase activity was observed in potato tuber tissue discs. Callus formation was no prerequisite for this increase; slicing and subsequent incubation of potato tuber tissue discs always led to an increase in alcohol dehydrogenase activity,
This paper describes the general applicability of a new pregelatinized starch product in directly compressible controlled-release matrix systems. It was prepared by enzymatic degradation of potato starch followed by precipitation (retrogradation), filtration and washing with ethanol. The advantages
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