Anticomplementary flavonoids from Galphimia glauca.

In continuation of pharmacological investigations on polyphenolic compounds from the aerial parts of Galphimia glauca, flavonol acyl glycosides and gallic acid derivatives were screened in the classical and alternative complement assay. A reduction of complement-induced hemolysis was measured for quercetin-3-O-(2″-galloyl)-β-D-glucoside by 91.3 ± 1.76% (100 μM) and by 76.69 ± 4.43% (50 μM), respectively, and for quercetin-3-O-(6″-galloyl)-β-D-glucoside by 74.3 ± 6.77% (50 μM). Isoquercitrin, hyperoside, gallic acid, and ellagic acid inhibited the hemolysis to a much lower extent. In the alternative pathway quercetin-3-O-(2″-galloyl)-β-D-glucoside and quercetin-3-O-(2″-galloyl)-β-D-glucoside exhibited weaker inhibitory effects than in the classical pathway.