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malus/arabidopsis

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The Artificial Promoter rMdAG2I Confers Flower-specific Activity in Malus.

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Genetic modifications of floral organs are important in the breeding of Malus species. Flower-specific promoters can be used to improve floral organs specifically, without affecting vegetative organs, and therefore developing such promoters is highly desirable. Here, we characterized two paralogs of

Malus domestica ADF1 severs actin filaments in growing pollen tubes

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A dynamic actin cytoskeleton is essential for pollen tube growth and germination. However, the molecular mechanism that determines the organisation of the actin cytoskeleton in pollen remains poorly understood. ADF modulates the structure and dynamics of actin filaments and influences the

Characterization of auxin-induced ARRO-1 expression in the primary root of Malus domestica.

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ARRO-1, a novel 2-oxoacid-dependent dioxygenase (2-ODD) is up-regulated during IBA-induced adventitious root formation in stem discs of Malus domestica. Analysis of ARRO-1's expression profile in the primary root of apple seedlings indicates that it is also highly up-regulated in the root in

Boosting carotenoid content in Malus domestica var Fuji by expressing AtDXR through an Agrobacterium mediated transformation method.

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Apple (Malus domestica) fruits accumulate negligible levels of carotenoids, antioxidant pigments that are precursors for vitamin A in humans. As Vitamin A deficiency is an important public health issue, we aimed at increasing carotenoids in apple by constitutively expressing the Arabidopsis thaliana

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis.

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NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are

The expression of a tubby-like protein from Malus domestica (MdTLP7) enhances abiotic stress tolerance in Arabidopsis.

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Tubby-like proteins (TLPs), characterized by a signature tubby domain, are widespread in plants and animals. To date, only plant TLPs involved in multifarious stress responses and male gametophyte development have been identified. However, studies on the molecular functions of plant

Transcriptome Analysis Reveals New Insights into MdBAK1-Mediated Plant Growth in Malus domestica.

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BAK1 effects on plant stress responses have been well documented, but little is known regarding its effects on plant growth. In this study, we functionally characterized MdBAK1. Overexpressing MdBAK1 in Arabidopsis thaliana and apple trees promoted growth. Longitudinal

A WD40-repeat gene from Malus x domestica is a functional homologue of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1.

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The WD40 repeat protein TRANSPARENT TESTA GLABRA1 (TTG1) is involved in a multitude of developmental and biochemical reactions in Arabidopsis thaliana such as the production of seed coat colour and mucilage, pigmentation by anthocyanins as well as the formation of trichomes and root hairs. In this

Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora.

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Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the

Dominance induction of fruitlet shedding in Malus x domestica (L. Borkh): molecular changes associated with polar auxin transport.

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BACKGROUND Apple fruitlet abscission is induced by dominance, a process in which hormones such as auxin, cytokinins and strigolactone play a pivotal role. The response to these hormones is controlled by transcription regulators such as Aux/IAA and ARR, whereas auxin transport is controlled by influx

Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica

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Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically

The 3-hydroxy-3-methylglutaryl-coenzyme A reductase 5 gene from Malus domestica enhances oxidative stress tolerance in Arabidopsis thaliana.

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3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) is the first rate-limiting enzyme regulating the synthesis of terpenoids upstream of the mevalonate (MVA) pathway. In higher plants, members of the HMGR genes families play an important role in plant growth and development and in response to

Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica).

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CONCLUSIONS MdCRY2 was isolated from apple fruit skin, and its function was analyzed in MdCRY2 transgenic Arabidopsis. The interaction between MdCRY2 and AtCOP1 was found by yeast two-hybrid and BiFC assays. Cryptochromes are blue/ultraviolet-A (UV-A) light receptors involved in regulating various

Genome-wide identification, evolution, and expression analysis of GATA transcription factors in apple (Malus×domestica Borkh.).

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Plant GATA transcription factors are type-IV zinc-finger proteins that play important regulatory roles in plant growth and development. In this study, we identified 35 GATA genes classified into four groups in the whole genome sequence of Malus domestica. A physiochemical property analysis indicated
Members of the sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family play central roles in the abscisic acid (ABA) signaling pathway and in mediating osmotic stress signaling and tolerance in plants. Previously, 12 full-length coding sequences that belong to SnRK2 gene family were
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