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vitis/− nicotine
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CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia.
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The CBF/DREB1 transcription factors control an important pathway for increased freezing and drought tolerance in plants. We report here the isolation of one CBF/DREB1-like gene, CBF4, from both freezing-tolerant wild grape (Vitis riparia) and freezing-sensitive cultivated grape (Vitis vinifera). The
Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera.
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Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years, most of these vectors are applied in annual or herbaceous plants. The aim of this work was to develop a
Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco.
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We report the identification of the inducer of CBF expression 1 (ICE1) from Vitis amurensis, an upstream transcription factor that regulates the transcription of CBF-like genes. The structure of the basic helix-loop-helix domain of VaICE1 is closely related to that of ICE1 in woody plants. This gene
Surface motility and associated surfactant production in Agrobacterium vitis.
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OBJECTIVE
Agrobacterium vitis is the causal agent of crown gall of grapevine. Surface motility (swarming), an important mechanism for bacterial colonization of new environments and a previously unknown behaviour of Ag. vitis, was demonstrated.
METHODS
Surface motility assays were performed on
The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco.
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Among the Agrobacterium T-DNA genes, rolB, rolC, orf13, orf8, lso, 6b and several other genes encode weakly homologous proteins with remarkable effects on plant growth. The 6b oncogene induces tumors and enations. In order to study its properties we have used transgenic tobacco plants that carry a
Regulation of long-chain N-acyl-homoserine lactones in Agrobacterium vitis.
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Homologs of quorum-sensing luxR and luxI regulatory genes, avsR and avsI, were identified in Agrobacterium vitis strain F2/5. Compared to other LuxI proteins from related species, the deduced AvsI shows the greatest identity to SinI (71%) from Sinorhizobium meliloti Rm1021. AvsR possesses
Characterization and phylogenetic diversity of Allorhizobium vitis isolated from grapevine in Morocco.
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Molecular characterization of DNA repair protein Ku70 from Vitis vinifera and its purification from transgenic tobacco.
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The DNA double strand break repair in plants is preferentially by non homologous end joining (NHEJ) pathway. A key protein of NHEJ pathway is Ku70. We have identified Ku70 homolog (VvKu70) from grapevine genome database. In this report we characterize a Ku70 homologue from Vitis vinifera cv. Mango.
Molecular Characterization and Overexpression of VpRPW8s from Vitis pseudoreticulata Enhances Resistance to Phytophthora capsici in Nicotiana benthamiana.
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RPW8 genes are atypical broad-spectrum genes that provide resistance to powdery mildew, downy mildew, the cauliflower mosaic virus in Arabidopsis thaliana, and powdery mildew in tobacco. They play important roles in basal plant pathogen defense. They also provide insights into a novel disease
Molecular characterization of VvSDIR1 from Vitis vinifera and its functional analysis by heterologous expression in Nicotiana tabacum.
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The proteins harboring really interesting new gene (RING) finger domains comprise a large family and play key roles in a variety of cellular processes. One among them is the tolerance to biotic and abiotic stresses in plants. In the present study, we characterize Vitis vinifera salt- and
The presence and characterization of a virF gene on Agrobacterium vitis Ti plasmids.
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Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show
The Effect of Transcription Factor MYB14 on Defense Mechanisms in Vitis quinquangularis-Pingyi.
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In the current study, we identified a transcription factor, MYB14, from Chinese wild grape, Vitis quinquangularis-Pingyi (V. quinquangularis-PY), which could enhance the main stilbene contents and expression of stilbene biosynthesis genes (StSy/RS) by
Pest categorisation of non-EU viruses and viroids of Vitis L
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Following a request from the EU Commission, the Panel on Plant Health addressed the pest categorisation of the viruses and viroids of Vitis L. determined as being either non-EU or of undetermined standing in a previous EFSA opinion. These infectious agents belong to different genera and are
Isolation and characterization of a chitinase gene VCH3 promoter from grapevine (Vitis amurensis).
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The 1216-bp 5' upstream region of the gene encoding a class III chitinase VCH3 was isolated from grapevine (Vitis amurensis Rupr.) by adaptor-PCR (GenBank accession number AF441123), and the transcriptional start site of the VCH3 gene was identified by primer extension, which corresponds to the
Molecular Cloning and Functional Characterization of a Dihydroflavonol 4-Reductase from Vitis bellula.
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Vitis bellula is a new grape crop in southern China. Berries of this species are rich in antioxidative anthocyanins and proanthocyanidins. This study reports cloning and functional characterization of a cDNA encoding a V. bellula dihydroflavonol reductase (VbDFR) involved in the biosynthesis of
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