The Role of Pyroptosis in Chronic Venous Disease
Keywords
Coimriú
Cur síos
Pyroptosis is a pro-inflammatory form of regulated cell death and is dependent on networks of extracellular fibers (NET,s), on the enzymatic activity of inflammatory proteases that belong to the family of cysteine-dependent aspartate-specific proteases (caspase), and cytokines. The role of inflammation has been previously demonstrated in chronic venous disease (CVD), which is a condition that affects up to 80% of adult population in western countries.
According to the Comprehensive Classification System for Chronic Venous Disorders (CEAP) divided into 6 Clinical stages:
C0: no visible or palpable signs of venous disease. C1: telangiectasies or reticular veins. C2: varicose veins. C3: edema. C4a: pigmentation and eczema. C4b: lipodermatosclerosis and atrophie blanche. C5: healed venous ulcer. C6: active venous ulcer.
The aim of this study is to evaluate the role of pyroptosis in the onset and progression of CVD.
The Investigators will select 200 participants with chronic venous disease (CVD) according to Clinical stage of CEAP (C1-C6 patients) [Group A] . The investigators will also select 200 healthy participants without CVD (C0) as controls [Group B].
Peripheral vein blood samples will be collected from both groups. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated between the two groups and between the subgroups of group A, according to clinical stage.
Dátaí
| Fíoraithe Deireanach: | 10/31/2018 |
| Cuireadh isteach den chéad uair: | 11/11/2018 |
| Clárú Measta Curtha isteach: | 11/14/2018 |
| Arna chur suas ar dtús: | 11/15/2018 |
| Nuashonrú Deireanach Curtha isteach: | 11/14/2018 |
| Nuashonrú Deireanach Postáilte: | 11/15/2018 |
| Dáta Tosaigh an Staidéir Iarbhír: | 11/11/2018 |
| Dáta Críochnaithe Bunscoile Measta: | 11/11/2019 |
| Dáta Críochnaithe an Staid Mheasta: | 11/11/2020 |
Coinníoll nó galar
Idirghabháil / cóireáil
Procedure: Peripheral blood draw
Céim
Grúpaí Láimhe
| Lámh | Idirghabháil / cóireáil |
|---|---|
| Group A - Participants with CVD Patients with chronic venous disease (CVD) will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples. | |
| Group B - Healthy Participants Voluntary healthy subjects will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples. |
Critéir Incháilitheachta
| Aois Incháilithe le haghaidh Staidéir | 18 Years Chun 18 Years |
| Gnéas Incháilithe le haghaidh Staidéir | All |
| Modh samplála | Non-Probability Sample |
| Glacann Oibrithe Deonacha Sláintiúla | Sea |
| Critéir | Inclusion Criteria: - Chronic Venous Disease (C1-C6 CEAP classification ) Exclusion Criteria: - Peripheral artery disease - Malignant Tumors - Severe cardiovascular and cerebrovascular disease - Liver and kidney failure |
Toradh
Bearta Toraidh Príomhúla
1. Pyroptosis evaluation 1 [2 years]
2. Pyroptosis evaluation 2 [2 years]
3. Pyroptosis evaluation 3 [2 years]
