Tissue culture systems of tobacco (Nicotiana tabacum L., Nicotiana rustica L.) are described, in which up to 5% and more nicotine accumulated on a dry weight basis. Maximum amounts of nicotine (5.3 %, yield 920 mg/1) were detected in mixotrophic green cell suspensions of a newly developed flue-cured
Alfalfa (Medicago sativa L. cv. Canadian No. 1), tobacco (Nicotiana tabacum L. var. humilis) and wheat (triticum monococcum L.) cells were grown in a defined, liquid medium containing either ammonium sulfate, L-glutamine or potassium nitrate as the sole nitrogen source, and the effects of the