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6 rezultatima
Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens.
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In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clone 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two
Isoprenoid regulation of cell growth: identification of mevalonate-labelled compounds inducing DNA synthesis in human breast cancer cells depleted of serum and mevalonate.
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Growth arrest induced by serum depletion and/or treatment with mevinolin (an inhibitor of mevalonate synthesis) in the human breast cancer cell line Hs578T was overcome by exogenous mevalonate, indicating that some product or metabolite of mevalonate may be involved in the mediation of
Dolichol-like lipids with stimulatory effect on DNA synthesis: substrates for protein dolichylation?
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Substantial evidence has suggested that a nonsterol product of mevalonic acid (MVA) is essential for the initiation of DNA synthesis in mammalian cells. Several possible isoprenoid candidates have been suggested, but the identity of this compound still remains unknown. In this study we have isolated
Effects of isoprenoids on growth of normal human mammary epithelial cells and breast cancer cells in vitro.
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The possible growth regulatory role of isoprenoids (mevalonate-derived products) in secondary cultures of normal human mammary epithelial cells (HMEC), as compared to the two human breast cancer cell lines Hs578T and MDA231, was investigated. All three cell types responded promptly to inhibitors of
Unfolded protein response is required in nu/nu mice microvasculature for treating breast tumor with tunicamycin.
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Up-regulation of the dolichol pathway, a "hallmark" of asparagine-linked protein glycosylation, enhances angiogenesis in vitro. The dynamic relationship between these two processes is now evaluated with tunicamycin. Capillary endothelial cells treated with tunicamycin were growth inhibited and could
Dynamic Function of DPMS Is Essential for Angiogenesis and Cancer Progression.
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Dolichol phosphate mannose synthase (DPMS) is an inverting GT-A-folded enzyme and classified as GT2 by CAZy. DPMS sequence carries a metal-binding DXD motif, a PKA motif, and a variable number of hydrophobic domains. Human and bovine DPMS possess a single transmembrane domain, whereas that from S.
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