5 rezultatima
In order to maximize staining, modifications of immunostaining methods have included proteolytic enzyme digestion of tissue. The authors performed a study of the effect of ficin in 110 paraffinized specimens, including tonsil, lymph nodes, benign vascular and nerve sheath tumors, and various
OBJECTIVE
In order to tracing the lung cancer, A monoclonal antibody was produced.
METHODS
The McAb, named SM-1, was specifically reacted with lung cancer confirmed by the immunopathological studies, then the McAb was digested into fragments by ficin and bromelain proteases.
RESULTS
Both of the
OBJECTIVE
Using monoclonal antibody (mAb) Fab' fragment to develop mAb immunoconjugates for cancer.
METHODS
Fab' fragment of mAb 3A5 was prepared by digestion of the antibody with pepsin and then reduced by dithiothreitol (DT), while Fab' fragment of mAb 3D6 was obtained by digestion of the antibody
In the latest two decades, the interest received by plant proteases has increased significantly. Plant enzymes such as proteases are widely used in medicine and the food industry. Some proteases, like papain, bromelain and ficin are used in various processes such as brewing, meat softening,
BACKGROUND
Vascular endothelial growth factor (VEGF) is a key regulator of physiologic and pathogenic angiogenesis in diseases such as cancer and diabetic retinopathy. It is known that cysteine proteases from plants, like bromelain and papain are capable to suppress inflammatory activation. Recent