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Thirty-two quarters, five of which harbored subclinical mastitis, were examined daily for one month. The usefulness of milk antitrypsin, BSA (bovine serum albumin), NAGase, somatic cells and bacteriological analysis in differentiating the inflamed quarters from the healthy control quarters was
The investigation was performed on 3 cows, sampled repeatedly before and during the initial 48 h of artificially induced, acute, clinical mastitis. The results of the investigation both augment and support those of earlier work on the levels and significant correlations of glucose, serum albumin and
An experimentally induced Escherichia coli infection of a bovine mammary gland resulted in a 30-fold increase in lactoferrin (Lf) concentration in the mammary secretion by 90 h postinoculation and a 4-fold increase in total daily production of Lf by 264 h postinoculation in the infected quarter. A
Foremilk, residual milk, and blood samples were studied for 10 days during acute mastitis episodes induced by endotoxin infused via the teat canal. Quarter milk and blood samples were collected frequently for 3 days after the infusion and thereafter once or twice daily. Leukocyte concentration in
From this investigation, it is apparent that owing to the superior diagnostic differentiating of udder conditions by means of the IDF/BSA criteria, a range of detailed data has become available which facilitates an unprecedented insight in the dynamic balancing during normal lactation of several
NAGase activity (NAGase) and serum albumin concentrations were determined in milk from 101 traditionally managed camels in the Sudan. NAGase, a lysosomal enzyme released from damaged epithelial cells as well as other somatic cells in milk, was recorded for quarter milk samples from camels (n = 353)
Cell concentration, electrical conductivity, and serum albumin concentration of milk were evaluated for predicting infection status of quarters in three herds. Probability of misclassifying quarters was lowest for cell concentration. For discriminating quarters infected with Staphylococcus aureus
This comparison of results from parallel investigations, conducted on the small herd model already discussed, has made it abundantly clear that during lactation, udder health as such depends on the 3 major determinants of intramammary epithelial integrity, somatic cellular defence and bacterial
The purpose in this experiment was to determine the relationship between subclinical mastitis and the estrous cycle. Subclinical mastitis was defined as a quarter in which milk had a somatic cell concentration greater than 500,000/ml and was bacteriologic culture-positive on 2 consecutive
The effect of combined administration of flunixin meglumine (FM) and nordihydroguaiaretic acid (NDGA) on milk prostaglandin F2 alpha (PGF2 alpha) and leukotriene B4 (LTB4) concentrations, and inflammatory indicators of bovine mastitis was examined. Mastitis was induced in six Holstein cows by the
Concentrations of prostaglandin F2 alpha (PGF2 alpha) and thromboxane B2 (TXB2) were evaluated in the milk of cows with naturally occurring (n = 3) and experimentally induced (n = 5) acute coliform mastitis. These arachidonic acid metabolites were measured by radioimmunoassay in unextracted milk.
During and after diapedesis, milk polymorphonu-clear neutrophils (PMN) release many proteases that have the potential of degrading extracellular matrix proteins and milk proteins. However, the kinetics of milk proteolysis during inflammation and the underlying mechanisms are poorly defined. The
Prostaglandin (PG) levels in milk and plasma samples from mastitic cows were determined by radioimmunoassay and compared with erythrocyte glutathione peroxidase (GSH-Px) and other relevant parameters in milk and blood. The overall levels of PGE2, PGF2 alpha and thromboxane B2 (TXB2) in milk were two