corydine/dactylicapnos

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Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis.

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Dactylicapnos scandens (D. Don) Hutch (Papaveraceae) is a well-known traditional Chinese herb used for treatment of hypertension, inflammation, bleeding and pain for centuries. Although the major bioactive components in this herb are considered as isoquinoline alkaloids (IQAs), little is known about

[Alkaloids from Dactylicapnos scandens Hutch].

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OBJECTIVE To investigate the alkaloids in the roots of Dactylicapnos scanden (D. Don) Hutch. METHODS The compounds were isolated by various column chromatographic methods. The structures were elucidated by spectroscopic analysis. RESULTS Eight compounds were isolated and identified as d-isocorydine

Preparative isolation of alkaloids from Dactylicapnos scandens using pH-zone-refining counter-current chromatography by changing the length of the separation column.

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pH-Zone-refining counter-current chromatography was successfully applied for the preparative separation of alkaloids from Dactylicapnos scandens. The two-phase solvent system was composed of petroleum ether-ethyl acetate-methanol-water (3:7:1:9, v/v), where 20 mM of triethylamine (TEA) was added to

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