jatropha tanjorensis/nicotine

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Functional Validation of JcWRKY2, a Group III Transcription Factor Toward Mitigating Salinity Stress in Transgenic Tobacco.

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The plants being sessile cannot escape from the adverse environmental stresses, hence get negatively affected in terms of their growth and yield. Transcriptional control simultaneously regulate different cellular processes, minimizing the deleterious effects of these stresses. The salicylic acid

The JcWRKY tobacco transgenics showed improved photosynthetic efficiency and wax accumulation during salinity.

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Salinity is one of the major factors negatively affecting crop productivity. WRKY transcription factors (TFs) are involved in salicylic acid (SA) mediated cellular reactive oxygen species homeostasis in response to different stresses, including salinity. Therefore, the effect of NaCl, NaCl + SA and

Targeting of castor bean glyoxysomal isocitrate lyase to tobacco leaf peroxisomes.

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The cDNA encoding castor bean endosperm isocitrate lyase (ICL) was expressed under the control of the promoter of the small subunit of pea ribulose bisphosphate carboxylase in transformed tobacco. ICL protein was detected using anti-ICL antibodies on immunoblots of total leaf protein extracts.

A New AP2/ERF Transcription Factor from the Oil Plant Jatropha curcas Confers Salt and Drought Tolerance to Transgenic Tobacco.

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Jatropha curcas L. is a drought and salt-tolerant oil plant widely used for various purposes and has considerable potential as a diesel/kerosene substitute or extender. Understanding the molecular mechanisms underlie that the response to various biotic and abiotic stresses of this plant could be

Potential of trap crops for integrated management of the tropical armyworm, Spodoptera litura in tobacco.

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The tropical armyworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae), is an important pest of tobacco, Nicotiana tabacum L. (Solanales: Solanaceae), in South China that is becoming increasingly resistant to pesticides. Six potential trap crops were evaluated to control S. litura on tobacco. Castor

Engineering storage capacity for volatile sesquiterpenes in Nicotiana benthamiana leaves.

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Plants store volatile compounds in specialized organs. The properties of these storage organs prevent precarious evaporation and protect neighbouring tissues from cytotoxicity. Metabolic engineering of plants is often carried out in tissues such as leaf mesophyll cells, which are abundant and easily

DNA-A of a highly pathogenic Indian cassava mosaic virus isolated from Jatropha curcas causes symptoms in Nicotiana benthamiana.

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Jatropha curcas mosaic disease (JcMD) is a newly emerging disease that has been reported in Africa and India. Here, we report the complete nucleotide sequence of a new Indian cassava mosaic virus isolate (ICMV-SG) from Singapore. Infection of ICMV-SG showed more severe JcMD in Jatropha curcas and

Overexpressing Jatropha curcas CBF2 in Nicotiana benthamiana improved plant tolerance to drought stress.

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Jatropha curcas is an important bioenergy oil plant, and often planted on barren land to save the area of arable land. It is significant to improve the adaptability of J.curcas to various abiotic stresses. In the present study, we transferred a J.curcas gene, encoding a CBF2 transcription factor,

Preproricin expressed in Nicotiana tabacum cells in vitro is fully processed and biologically active.

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Ricin, the highly toxic glycoprotein expressed in the endosperm of castor seeds, is composed of a galactose-binding lectin B chain (RTB) disulfide linked to a RNA N-glycosidase A chain (RTA). Chemically modified ricin has been conjugated to monoclonal antibodies and used for targeted therapy of

Overexpression of the Jatropha curcas JcERF1 gene coding an AP2/ERF-type transcription factor increases tolerance to salt in transgenic tobacco.

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The JcERF1 gene, which is related to the ERF family (ethylene responsive factor coding genes), was isolated and characterized from the oil tree Jatropha curcas. The JcERF1 protein contains conserved an AP2/EREBP DNA-binding domain of 58 amino acid residues. The JcERF1 gene could be induced by

Differential regulation of the expression in transgenic tobacco of the gene for beta-glucuronidase under the control of the 5'-upstream regions of two catalase genes from castor bean.

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The regulatory functions of the 5'-flanking regions of two genes for catalase (cat1 and cat2) from castor bean were analyzed in transgenic tobacco plants that carried fusion constructs that included the gene for beta-glucuronidase (GUS) for Escherichia coli. Dry mature seeds from transgenic plants

The modified castor bean catalase intron is incompletely spliced in tobacco and Arabidopsis.

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In an attempt to insert the modified castor bean catalase intron (mCBC intron) into the coding sequence of the Cre recombinase gene, we found that the mCBC intron was not completely spliced from the resulting iCre gene in tobacco and Arabidopsis. Sequencing and allele-specific PCR analyses indicated

Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco.

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Deletion analysis of the promoter region of a gene for catalase, cat2, from castor bean (Ricinus communis) was performed to identify the cis-regulatory elements responsible for the expression of a beta-glucuronidase (GUS) fusion gene during seed formation and postembryonic development in transgenic

Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction.

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Ricin B (RTB), the non-toxic lectin subunit of ricin, is a promising mucosal adjuvant and carrier for use in humans. RTB fusion proteins have been expressed in tobacco hairy root cultures, but the secreted RTB component of these proteins was vulnerable to protease degradation in the medium.

Processing of preproricin in transgenic tobacco.

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The plant protein toxin ricin has found widespread application as a potential therapeutic agent for many human diseases and in disease-model systems such as those involving apoptosis. Genetic engineering and expression of the complete two-polypeptide chain toxin have only been possible in plants,

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