Participants will be provided with control chicken-meat and control eggs, and will be requested to eat at least three portions/week of the control chicken-meat, and to eat at least three control eggs/week, for 6 months.

Participants will be provided with control chicken-meat and omega-3-PUFA enriched eggs, and will be requested to eat at least three portions/week of the control chicken-meat, and to eat at least three omega-3-enriched eggs/week, for 6 months.

Participants will be provided with omega-3-PUFA enriched chicken-meat and control eggs, and will be requested to eat at least three portions/week of the omega-3-PUFA enriched chicken-meat, and to eat at least three control eggs/week, for 6 months.

Participants will be provided with omega-3-PUFA enriched chicken-meat and omega-3-PUFA enriched eggs, and will be requested to eat at least three portions/week of the omega-3-PUFA enriched chicken-meat, and to eat at least three omega-3-PUFA enriched eggs/week, for 6 months.

Healthy Diet

Control Chicken-meat

Control Eggs

Omega-3-PUFA Enriched Chicken-meat

Omega-3PUFA Enriched Eggs

Royal College of Surgeons at Beaumont Hospital

Impacts of Omega-3-PUFA Enriched Chicken-meat and Eggs in Healthy Humans

A Study of the Absorption, Distribution and Cardiovascular Benefits for Humans of Eating Chicken-meat and Eggs Enriched With Omega-3 PUFAs Over a 6-month Period

Sum of EPA and DHA expressed as percentage of total fatty acids in erythrocyte membranes

Sum of plasma levels of EPA and DHA (microgram/gram)

Mean 24-hour ambulatory systolic blood pressure (mmHg)

Mean 24-hour ambulatory diastolic blood pressure (mmHg)

Mean 24-hour ambulatory heart rate (beats/minute)

High-sensitivity C-reactive protein (mg/L)

Urinary thromboxane/creatinine ratio (pg/mg creatinine)

(Z Verbal Fluency (Letter Fluency) + Z Verbal Fluency (Category Fluency) + Z Verbal Fluency (Switching Fluency) - Z Trail Making Test (Number Letter Switching - [(Number Sequencing + Letter Sequencing)/2]) -Z Colour Word Interference (Inhibition - [(Colour Naming + Word Reading)/2]) - Z Colour Word Interference (Inhibition Switching - [(Colour Naming + Word Reading)/2]))/ 6

(Z Word List (Immediate Recall) + Z Word List (Delayed Recall) + Z Word List (Recognition) + Z Logical Memory (Immediate Recall) + Z Logical Memory (Delayed Recall) + Z Logical Memory (Recognition) ) /6

(Z Complex Figure (Immediate Recall) + Z Complex Figure (Delayed Recall))/2

(Z Trail Making Test (Visual Scanning) + Z Trail Making Test (Number Sequencing) + Z Trail Making Test (Letter Sequencing) + Z Colour Word Interference (Colour naming) + Z Colour Word Interference (Word Reading)) /5) * - 1

(Z Finger Tapping (Dominant Hand) + Z Finger Tapping (Non-Dominant Hand)) /2

All de-identified individual participant data that underlie the results in a publication, and applicable supporting clinical trial documents, may be available upon request.

Alice Stanton

Professor of Cardiovascular Therapeutics

Devenish Nutrition, Belfast, United Kingdom.

Beaumont Hospital

University College Dublin

This will be a controlled, double-blind randomized study. Using a 2X2 factorial design, 160 participants will be randomized to eat at least three portions/week of omega-3-PUFA enriched (or control) chicken-meat, and to eat at least three omega-3-PUFA enriched (or control) eggs/week, for 6 months.
Numbers in each treatment group are indicated below; Control chicken-meat & Control Eggs (n=40) Control chicken-meat & Omega-3 PUFA enriched Eggs (n=40) Omega-3 PUFA enriched chicken-meat & Control Eggs (n=40) Omega-3 PUFA enriched chicken-meat & Omega-3 PUFA enriched Eggs (n=40)

Randomisation and masking The randomisation schedule was computer generated. Separate sealed opaque envelopes were prepared for male and female participants. All investigators involved in participant recruitment, data collection and entry, sample collection, processing and analysis, will be masked to the randomisation groups until data entry is completed and the database is locked.

Subcutaneous adipose tissue, serum, plasma, ''buffycoat'', erytrocytes and urine is stored in nitrogen vapour at the biobank of the Diet, Cancer and Health cohort study. Also, whole blood in filter paper and toenail clippings has been stored within the biobank.

Acute Myocardial Infarction

Ischemic Stroke

Peripheral Artery Disease

Exposure to dietary intake of alpha-linolenic acid (ALA) will be assessed using a detailed food frequency questionaire filled in by participants included in the Diet, Cancer and Health cohort at baseline. Exposure to adipose tissue content of ALA will be determined by gas chromatography in all incident cases and in subcohort of 3500 participants randomly drawn from the total cohort.

Alpha-linolenic acid

Alpha-linolenic Acid and the Risk of ASCVD

Alpha-linolenic Acid and the Risk of Atherosclerotic Cardiovascular Disease

Christian Sørensen Bork

Dr. Christian Sørensen Bork

Aalborg University Hospital

Danish Heart Foundation

Omega-3 fatty acid ethyl esters, 2 soft gels of total 1000mg (660mg EPA +340mg DHA)/day.

210mg of Omega-7 fatty acid and 1000mg (660mg EPA +340mg DHA) of Omega-3

Inflammation

Ingestion of omega 7 + 3 daily for 8 weeks.

Omega 7 + 3

Omega 3

Placebo

Appalachian State University Human Performance Lab, North Carolina Research Campus

Omega 7 + 3 Combination and Systemic Inflammation

Influence of Omega 7 + 3 Combination on Systemic Inflammation and Plasma Lipoproteins in Women: a 2-month, Double Blind, Placebo-Controlled, Randomized Community Trial

Effects of 8-weeks supplementation with omegas 7 and 3 fats combination versus omega-3 fats and placebo on systemic inflammation (CRP and cytokine panel).

Effects of 8-weeks supplementation with omegas 7 and 3 fats combination versus omega-3 fats and placebo on plasma lipoprotein panel.

linolenic acid/stroke

Impacts of Omega-3-PUFA Enriched Chicken-meat and Eggs in Healthy Humans

Alpha-linolenic Acid and the Risk of ASCVD

Omega 7 + 3 Combination and Systemic Inflammation

Effects of California Walnuts on Vascular Function in Postmenopausal Women

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