oenanthotoxin/oenanthe crocata

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Identification of oenanthotoxin and related compounds in hemlock water dropwort poisoning.

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The case histories are presented of three separate incidents of poisoning by ingestion of Hemlock Water Dropwort tubers (Oenanthe crocata). Two of these cases involved a fatality. An analytical profile is provided for oenanthotoxin, the major toxic principal of the tubers. Chromatographic,

Application of liquid chromatography-mass spectrometry to the investigation of poisoning by Oenanthe crocata.

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Liquid chromatography-mass spectrometry (LC-MS) analysis of methanol extracts of Oenanthe crocata roots revealed that oenanthotoxin co-eluted with another major polyalkyne, 2,3-dihydro-oenanthotoxin, using the existing high performance liquid chromatography (HPLC) method (isocratic elution from C18

Hemlock water dropwort poisoning.

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Severe plant poisoning is relatively uncommon in adults. We report two adults who ingested hemlock water dropwort roots, having mistaken them for wild parsnip. One developed prolonged convulsions, severe metabolic acidosis and respiratory distress requiring mechanical ventilation. The

Inhibitory effects of oenanthotoxin analogues on GABAergic currents in cultured rat hippocampal neurons depend on the polyacetylenes' polarity.

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Oenanthotoxin (OETX) and dihydro-OETX are polyacetylenic diols occurring in Oenanthe crocata and are known to exert proconvulsant effects. We have recently demonstrated that these compounds downregulated GABAergic currents (Appendino et al., 2009) and that OETX induced open channel block and

Fluorimetric determination of oenanthotoxin.

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Oenanthotoxin as crude yellow crystals was obtained, after the extraction and treatment from Oenanthe crocata L. fresh roots. The fluorescence response of oenanthotoxin solutions undergo changes, depending on the solvent used. Chloroformic solutions of oenanthotoxin showed a maximum fluorescent

Falcarindiol allosterically modulates GABAergic currents in cultured rat hippocampal neurons.

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Falcarindiol (1), a C-17 polyacetylenic diol, shows a pleiotropic profile of bioactivity, but the mechanism(s) underlying its actions are largely unknown. Large amounts of 1 co-occur in water hemlock (Oenanthe crocata) along with the convulsant polyacetylenic toxin oenanthotoxin (2), a potent

Polyacetylenes from sardinian Oenanthe fistulosa: a molecular clue to risus sardonicus.

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An investigation of Oenanthe fistulosa from Sardinia afforded oenanthotoxin (1a) and dihydrooenanthotoxin (1b) from the roots and the diacetylenic epoxydiol 2 from the seeds. The absolute configuration of 1a and 1b was established as R by the modified Mosher's method, and the structure of 2 by

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