onchocerciasis/phosphatase

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Histochemical enzyme variation in Onchocerca volvulus microfilariae from rain-forest and Sudan-savanna areas of the Onchocerciasis Control Programme in West Africa.

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Histochemical staining methods for acid phosphatase were used to study the differences among microfilariae of various West African strains of Onchocerca volvulus in both forest and Sudan-savanna onchocerciasis zones. The results have shown statistically significant differences in the staining

Detection, purification and characterisation of a secretory alkaline phosphatase from Onchocerca species.

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An Onchocerca secretory alkaline phosphatase (E.C. 3.1.3.1) of molecular weight 90 kDa when in crude extract, but which dimerises to about 180 kDa upon purification, was detected, purified and characterised. The enzyme was found to be secreted by both O. ochengi and O. volvulus worms. It was shown

The river blindness drug Ivermectin and related macrocyclic lactones inhibit WNT-TCF pathway responses in human cancer.

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Constitutive activation of canonical WNT-TCF signaling is implicated in multiple diseases, including intestine and lung cancers, but there are no WNT-TCF antagonists in clinical use. We have performed a repositioning screen for WNT-TCF response blockers aiming to recapitulate the genetic blockade

Some observations on onchocerciasis including sowda in the Yemen Arab Republic.

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A pilot study on onchocerciasis and sowda was carried out in the south-western region of the Yemen Arab Republic. Of 61 persons examined in eight villages 35 (= 57%) were found positive for microfilariae by the skin-snip method, 13 had typical manifestations of sowda, 17 had other

A monoclonal antibody-based immunodiagnostic assay for onchocerciasis.

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Five murine monoclonal antibodies raised against Onchocerca volvulus cuticular extracts and termed MOVs (1-4 and 6) were selected based on reactivity with O. volvulus cryosections, and non-reactivity with cryosections of human skin and/or nodular tissue. Two others MOVs 5 and 7 reacted with both.

Histochemical enzyme-staining patterns of onchocerca volvulus microfilariae and their occurrence in different onchocerciasis areas.

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Histochemical differentiation of Onchocerca volvulus microfilariae from 164 patients in West African rain-forest (Liberia), Sudan-savanna (Upper Volta), Guatemala and the Yemen has been carried out using a staining method for the demonstration of acid phosphatase. Intrauterine microfilariae showed

Onchocerciasis in British cattle: a study of Onchocerca gutturosa and O. lienalis in North Wales.

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Onchocerca gutturosa and O. lienalis infections in British cattle were studied by examination of cattle post-mortem originating from North Wales and Cheshire (north west England). In 463 adult animals, the microfilarial (mf) prevalence was 28.5%. In 95.3% of the mf infected animals, gravid worms

Specific and cross-reacting antibodies in human responses to Onchocerca volvulus and Dracunculus medinensis infections.

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Immunoelectroblotting and enzyme-linked immunosorbent assay were used to identify non-cross-reacting antigenic components of Dracunculus medinensis and the filarial worms Onchocerca volvulus, Loa loa, Wuchereria bancrofti, Brugia malayi, and Mansonella ozzardi. Parasite specific serodiagnostic ELISA

Suramin inhibits cell growth and glycolytic activity and triggers differentiation of human colic adenocarcinoma cell clone HT29-D4.

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Suramin, a drug used in the treatment of trypanosomiasis and onchocerciasis inhibits growth factor-induced mitogenesis. In the present report, we show that suramin inhibits the growth of human colic adenocarcinoma cells HT29-D4 and rapidly induces their differentiation into enterocyte-like cells. As

The development of Onchocerca ochengi in surrogate temperate Simuliidae, with a note on the infective larva.

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Laboratory reared nulliparous female flies of six temperate species of Simuliidae were examined for their susceptibility to infection with Onchocerca ochengi by intrathoracic injection of cryopreserved skin microfilariae obtained from cattle in Mali. Three species (S. equinum, S. ornatum and S.

Histochemical differentiation of filarial larvae found in Simulium damnosum s.l. in West Africa.

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The azo-dye method for the histochemical demonstration of acid phosphatase activity was used to differentiate filarial larvae within and outside th area of the Onchocerciasis Control Programme (OCP) in natural infections of female S. damnosum s.l. caught in West Africa. The histochemical patterns of

Preliminary studies on the histochemical differentiation of strains of Onchocerca volvulus microfilariae in Togo.

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Skin snips were taken from 75 people living in four villages of northern Togo. The 7824 microfilariae that emerged were examined by staining for the presence of acid phosphatase. Four distinct patterns of enzyme staining were observed, and descriptions of the stained microfilariae are given. The

Hydrolytic activity of an onchocercoma.

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The onchocercoma or nodule produced by the nematode Onchocerca volvulus (Filarioidea) in the skin of patients suffering from onchocerciasis has not been examined by histochemical techniques. In this work we have used histochemical techniques to evaluate 5 hydrolytic enzymes, namely phosphatases,

The suramin-treated rat as a model of mucopolysaccharidosis. Variation in the reversibility of biochemical and morphological changes among different organs.

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We have examined the reversibility of the biochemical and pathological changes induced in the spleen, kidney and lung of the suramin-treated rat which we have previously proposed as a useful model of the human condition, mucopolysaccharidosis (MPS). Rats were injected with a single intravenous dose

A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus.

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BACKGROUND Serological assays for human IgG4 to the Onchocerca volvulus antigen Ov16 have been used to confirm elimination of onchocerciasis in much of the Americas and parts of Africa. A standardized source of positive control antibody (human anti-Ov16 IgG4) will ensure the quality of surveillance

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