phytohemagglutinin/lafyèv

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[Skin response to phytohemagglutinin in patients with typhoid fever, bronchial asthma and Hodgkin's disease].

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The skin response to phytohemagglutinin was investigated as an index of cellular immunity in group of 50 children made up of 11 with thypoid fever, 13 with bronchial asthma type I, 15 with Hodgkin's disease and 11 controls. A mild but statistically significant (p less than 0.005) depression was

Specific role of each human leukocyte type in viral infections. 3. 17D yellow fever virus replication and interferon production in homogeneous leukocyte cultures treated with phytohemagglutinin.

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In vivo and in vitro effects of moderately virulent African swine fever virus on mitogenesis of pig lymphocytes.

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Six pigs were infected oro-nasally with a moderately virulent African swine fever (ASF) virus from the Dominican Republic (DR II). The effect of virus infection on the pig's immune system was tested by measuring peripheral leucocyte numbers and the ability of mononuclear leucocytes (MNL) to respond

[Cellular and humoral immunity in general exogenous hyperthermia].

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The proliferative activity of splenic cells (SC) to phytohemagglutinin (PHA), concanavalin A (Con A), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and alloantigens, as well as the immune response to sheep red cells (SRS) were investigated in mice undergoing hyperthermia. There was an increase in

Tumor necrosis factor in familial Mediterranean fever.

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OBJECTIVE The pleiotropic inflammatory effects of tumor necrosis factor (TNF) prompted a study of this cytokine in familial Mediterranean fever (FMF), a recurrent polyserositis of unknown etiology. METHODS Thirty-six asymptomatic and 24 patients with acute FMF were studied and compared with 20

The effect of in vivo hyperthermia on selected lymphokines in man.

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We have previously demonstrated that artificially induced hyperthermia in man enhances the subsequent production of interferon gamma (IFN-gamma) in isolated leukocyte cultures. The mechanism(s) responsible for this response may involve changes in the circulating lymphocyte populations and may also

[The pathogenetic role of peripheral T-lymphocytes activation-induced cell death in hemorrhagic fever with renal syndrome].

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OBJECTIVE To study the activation-induced cell death (AICD) in peripheral blood T-lymphocytes (PBL-T) from patients of hemorrhagic fever with renal syndrome (HFRS) and the effect of AICD on the pathogenesis of HFRS. METHODS The PBL-T of patients with HFRS were isolated by negative selection with

Depression of CD4 T cell subsets and alteration in cytokine profile in boutonneuse fever.

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Interferon (IFN)-gamma, interleukin (IL)-10, IL-6, and tumor necrosis factor (TNF)-alpha were significantly increased in sera from Sicilian patients with acute boutonneuse fever (BF) compared with those of healthy controls. IFN-gamma levels dropped sharply within the second week after infection.

Increase in activated T cells and reduction in suppressor/cytotoxic T cells in acute rheumatic fever and active rheumatic heart disease: a longitudinal study.

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In a prospective study, patients with acute rheumatic fever (ARF) and active rheumatic heart disease (ARHD) showed a significant increase in CD4+ T cells, CD22+ B cells, and CD4:CD8 cell ratio but a significant decrease in the percentages of CD8+ and CD3+ T lymphocytes compared with patients with

Effect of local hyperthermia on lymph immune cells and lymphokines of normal human skin.

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The effects of 3 h lasting local hyperthermia on immune cell traffic through the normal human skin to afferent lymphatics, cell phenotypes, responsiveness, and stimulatory properties were studied in eight men. Cells were harvested from lymph drained from foot skin. Heating the skin in a water bath

Effect of hyperthermia on the survival of normal human peripheral blood mononuclear cells.

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Human peripheral blood mononuclear cells from normal healthy volunteers were exposed to elevated temperatures of 41-43 degrees for up to 6 hr. Thereafter, the cells were stimulated with phytohemagglutinin in vitro in order to measure indirectly the surviving fraction. DNA replication in heated cells

Hyperthermia and human leukocyte functions: effects on response of lymphocytes to mitogen and antigen and bactericidal capacity of monocytes and neutrophils.

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It has recently been demonstrated that fever, or hyperthermia, results in enhanced survival of lizards infected by Aeromonas hydrophila. In the present study, the effects of hyperthermia on certain immune functions were assayed in vitro with purified human leukocytes. Lymphocyte transformation

Correlation between B-cell mitogenicity and immunosuppressor effects of a protein released by porcine monocytes infected with African swine fever virus.

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Virus-free supernatants of cultured swine monocytes infected by African swine fever virus (ASFV) suppressed in vitro proliferation of porcine and human blood mononuclear cells in response to phytohemagglutinin and the in vivo primary immune response of C57BL/6 mice against sheep RBC. The

Simultaneous analysis of DNA replication and apoptosis during treatment of HL-60 cells with camptothecin and hyperthermia and mitogen stimulation of human lymphocytes.

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A new method is described which combines the identification of DNA replicating and apoptotic cells in a single measurement by flow cytometry. The detection of DNA replicating cells is based on incorporation of 5-bromo-2'-deoxyuridine or 5-iodo-2'-deoxyuridine, followed by selective photolysis at the

Demonstration of a circulating suppressor factor of thymocyte proliferation during endotoxin fever in humans.

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Sub-pyrogenic levels of human leukocytic pyrogen (LP) have been shown to enhance phytohemagglutinin-induced murine thymocyte proliferation. It was concluded that LP is similar to lymphocyte-activating factor (LAF). Since endotoxin stimulates the production of LP and LAF, we attempted to employ in

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