pinus/protease

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On the stability of isocitrate lyase from Pinus pinea.

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Isocitrate lyase is a key catalyst of the glyoxylate cycle. A feature of the enzyme from higher plants is the high instability, that causes innumerable problems in working for characterization of the enzyme. The present communication demonstrates that the optimal conditions for the storage of

[Changes in proteins and proteases during artificial aging in seeds of Pinus pinea].

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Changes in the germination-rate and the biochemical differences in soluble proteins and proteases in artificially aged Pinus pinea seeds have been investigated. In the aged seeds, soluble proteins decrease during the germination process, parallel to increased levels of proteases activities in both

PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster.

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We have isolated and characterized a complete retrotransposon sequence, named PpRT1, from the genome of Pinus pinaster. PpRT1 is 5,966 bp long and is closely related to IFG7 gypsy retrotransposon from Pinus radiata. The long terminal repeats (LTRs) have 333 bp each and show a 5.4% sequence

A novel Otubain-like cysteine protease gene is preferentially expressed during somatic embryogenesis in Pinus radiata.

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OTUBAINS are a recently discovered family of cysteine proteases that participate in the ubiquitin pathway. These proteins were originally described in animal systems and are involved in removing the ubiquitin chain attached to a protein destined for degradation. In a cDNA-AFLP screen designed to

Purification and characterization of the 7S vicilin from Korean pine (Pinus koraiensis).

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Pine nuts are economically important as a source of human food. They are also of medical importance because numerous pine nut allergy cases have been recently reported. However, little is known about the proteins in pine nuts. The purpose of this study was to purify and characterize pine nut storage

An experimental test of dietary enzyme modulation in pine warblers Dendroica pinus.

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Modulation of gut function is important in an ecological and evolutionary context because it likely determines what food items an animal can and cannot eat. We examined how diet affects activity of digestive enzymes in an omnivorous bird, the pine warbler (Dendroica pinus). Pine warblers were fed

[Effects of elevated CO2 on soil hydrolase activities associated with Pinus koraiensis seedlings].

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In this study, Pinus koraiensis seedlings were grown under elevated CO2 (500 and 700 micromol x mol(-1)), and the hydrolase activities in 0 - 10 cm soil layer were determined. The results showed that compared with those under ambient condition, the activities of soil protease, urease, amylase and

Proteolytic activity in relation to seasonal cambial growth and xylogenesis in Pinus banksiana.

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Proteolytic activity in the cambial zone and developing xylem of Pinus banksiana Lamb. was investigated over an annual cycle of growth and dormancy. Highest proteolytic activity was associated with the most active period of primary-wall radial expansion of cambial derivatives, in early spring,

Inhibitory effects of Pinus massoniana bark extract on hepatitis C virus in vitro.

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BACKGROUND Given that Pinus massoniana Lamb (Pinaceae) bark extract (PMBE) is a safe and non-toxic flavonoid found abundantly in nature, it was considered a promising novel candidate agent in the treatment of virus infection. OBJECTIVE Experiments were conducted to assay the antiviral character of

Involvement of VNS NAC-domain transcription factors in tracheid formation in Pinus taeda.

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Vascular plants have two types of water-conducting cells, xylem vessel cells (in angiosperms) and tracheid cells (in ferns and gymnosperms). These cells are commonly characterized by secondary cell wall (SCW) formation and programmed cell death (PCD), which increase the efficiency of water

Identification and expression of the chloroplast clpP gene in the conifer Pinus contorta.

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The clpP gene from the conifer Pinus contorta was identified and isolated from a chloroplast genomic library by heterologous hybridisation to the second exon of the chloroplast clpP gene in tobacco. DNA sequencing of two overlapping clones revealed an uninterrupted 615 bp open-reading frame with 41

NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.

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Plants have developed a complex defense response system against pests and pathogens. Defensins, produced by plants as part of their innate immune response, form the family of small, basic, cysteine-rich proteins with activity primarily directed against fungal pathogens. In addition, plant defensins

Integrated Physiological, Proteomic, and Metabolomic Analysis of Ultra Violet (UV) Stress Responses and Adaptation Mechanisms in Pinus radiata.

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Globally expected changes in environmental conditions, especially the increase of UV irradiation, necessitate extending our knowledge of the mechanisms mediating tree species adaptation to this stress. This is crucial for designing new strategies to maintain future forest productivity. Studies

Characterization of the orthodox Pinus occidentalis seed and pollen proteomes by using complementary gel-based and gel-free approaches.

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This work presents an analysis of Pinus occidentalis pollen and seed proteomes, in which both gel-based and gel-free approaches have been used. Proteins were extracted from P. occidentalis seeds and pollen by using the TCA/acetone/phenol precipitation protocol, and protein extracts were subjected to

Transcriptome analysis of Pinus monticola primary needles by RNA-seq provides novel insight into host resistance to Cronartium ribicola.

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BACKGROUND Five-needle pines are important forest species that have been devastated by white pine blister rust (WPBR, caused by Cronartium ribicola) across North America. Currently little transcriptomic and genomic data are available to understand molecular interactions in the WPBR

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