pseudorabies/phosphatase

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[Cytological and cytochemical studies of cell cultures infected with the avirulent viral mutant MK 35 of the pseudorabies virus].

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Cytological and cytochemical studies were carried out of cell cultures of chick embryo fibroblasts infected with the vaccinal mutant strain MK 35 (4.8 X 10(7) PFU) of the Pseudorabies virus. It was found that the first cytologic changes in the infected cultures presented definite focal character. In

Magnetic beads-based chemiluminescent assay for ultrasensitive detection of pseudorabies virus.

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A rapid, ultrasensitive and economical Pseudorabies virus (PRV) detection system based on magnetic beads (MBs) and chemiluminescence was developed in this paper. The carboxyl functionalized MBs (MBs-COOH) were covalently coupled with aminated DNA probes for capturing PRV biotinylated amplicon, the

The IE180 protein of pseudorabies virus suppresses phosphorylation of translation initiation factor eIF2α.

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We have previously shown that the porcine alphaherpesvirus pseudorabies virus (PRV) efficiently interferes with phosphorylation of the eukaryotic translation initiation factor eIF2α. Inhibition of phosphorylation of eIF2α has been reported earlier for the closely related alphaherpesvirus herpes

Chemiluminescent detection of amplified pseudorabies virus gp50 DNA with immobilized probes on microtiter wells.

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A highly specific and sensitive PCR assay for the envelope glycoprotein gp50 has been developed for the detection of PRV-DNA sequences. Primer pairs from PRV gp50 gene were used with the enzyme uracil N-glycosylase and dUTP instead dTTP to prevent contamination due to PCR product carry-over.

Retinal transplants restore visual responses: trans-synaptic tracing from visually responsive sites labels transplant neurons.

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This study aimed to test the hypothesis that visual responses in the superior colliculus (SC) originate from synaptic connections between fetal retinal transplants and degenerating host retinas. Sheets of embryonic day 19 rat retina expressing human placental alkaline phosphatase were transplanted

Detection of porcine parvovirus using nonradioactive nucleic acid hybridization.

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Nonradioactive slot blot hybridization assays were established for the detection of porcine parvovirus (PPV), using either a digoxigenin-labeled DNA probe or a biotinylated RNA probe. All probes were prepared from a 3.3-kb Pst1-EcoR1 DNA fragment of the NADL8 isolate of PPV. The sensitivity and

A restriction cleavage and transfection system for introducing foreign DNA sequences into the genome of a herpesvirus.

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This report describes a simple and efficient system for construction of recombinant pseudorabies (Aujeszky's disease) virus (PrV) which is based on the use of a unique restriction site inserted into the viral genome. This system enables the recovery of genetically modified viruses without screening

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