Shanghai kou qiang yi xue = Shanghai journal of stomatology 2019-Aug
[Antibacterial activity of erythritol on periodontal pathogen].
Միայն գրանցված օգտվողները կարող են հոդվածներ թարգմանել
Մուտք / Գրանցվել
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METHODS
Pg, Aa, Av were anaerobically cultured (80%N2, 10%CO2, 10%H2) at 37℃ in 2, 4, 8, 16, 32, 64, 128 g/L erythritol- BHI mixture groups (experimental groups) and BHI groups (control group). The lowest erythritol concentration without turbidity or precipitation was the minimum inhibitory concentration. Pg was cultured in MIC, 1/2, 1/4, 1/8 MIC erythritol- BHI mixture groups (experimental groups) and BHI groups (control group). Each kind of bacteria in each concentration group was centrifugalled and cleaned before added into DMEM. The mixed suspension was co-cultured with the periodontal ligament cells in four generations for 24 hours, the supernatan was removed , then the total RNA in cracking cells was extracted and reversing transcription. At last, the relative expression of IL-1, IL-6, TNF-a was detected real-time fluorescent quantitative PCR. The data were analyzed with SPSS19.0 software package.