Low-calorie Sweeteners and Adipose Signaling
Kata kunci
Abstrak
Deskripsi
The proposed study will investigate LCS-induced changes in metabolically "at-risk" young adults, under conditions that reflect typical beverage consumption. We will first identify molecular pathways potentially affected by LCS (adipose transcriptomics via global RNAseq) and then identify links between LCS-induced gene expression changes and circulating levels of key metabolic biomarkers and clinical outcomes. We will perform subcutaneous adipose biopsies before and after consumption of 12 ounces of diet soda three times daily for eight weeks. Within-subjects changes in adipose gene expression will be evaluated using RNAseq. We will then correlate transcriptomic changes with circulating levels of key inflammatory and metabolic biomarkers, to provide mechanistic insight into which pathways drive clinically-relevant LCS-induced metabolic impairments. We will measure glucose, insulin, inflammatory markers and adipose-derived hormones before and after eight weeks of LCS exposure.
tanggal
| Terakhir Diverifikasi: | 03/31/2019 |
| Pertama Dikirim: | 04/18/2017 |
| Perkiraan Pendaftaran Telah Dikirim: | 04/18/2017 |
| Pertama Diposting: | 04/23/2017 |
| Pembaruan Terakhir Dikirim: | 02/13/2020 |
| Pembaruan Terakhir Diposting: | 02/16/2020 |
| Tanggal Mulai Studi Sebenarnya: | 12/31/2016 |
| Perkiraan Tanggal Penyelesaian Utama: | 12/30/2018 |
| Perkiraan Tanggal Penyelesaian Studi: | 12/30/2018 |
Kondisi atau penyakit
Intervensi / pengobatan
Other: Diet Soda
Tahap
Kelompok Lengan
| Lengan | Intervensi / pengobatan |
|---|---|
| Experimental: Diet Soda Subjects will be asked to consume a diet soda three times daily for eight weeks. | Other: Diet Soda Subjects will be asked to consume diet soda three times daily for eight weeks. |
Kriteria kelayakan
| Usia yang Layak untuk Belajar | 18 Years Untuk 18 Years |
| Jenis Kelamin yang Layak untuk Belajar | Female |
| Menerima Relawan Sehat | Iya |
| Kriteria | Inclusion Criteria: 1. Female 2. 18-25 years of age 3. Overweight and mild to moderate obesity (25 ≤ body mass index (BMI) <35 kg/m2) 4. Consumption of <1 food/beverage with sucralose and/or acesulfame-potassium per month 5. Able and willing to comply with all study procedures for the duration of the study Exclusion Criteria: 1. Overt diabetes 2. Renal/gastrointestinal conditions 3. Pregnant/lactating 4. Recent significant weight change 5. Acute illness (w/fever) 6. Antibiotics in past 3 months 7. Use of illegal drugs/condition limiting ability to give consent. |
Hasil
Ukuran Hasil Utama
1. Transcriptomics [Pre/post 8 weeks of diet soda]
