Process for preventing diarrhea in animals

FIELD OF THE INVENTION

This invention relates to a pharmaceutical composition for animals excluding humans which is used alone or in admixture with water or with a feed stuff.

BACKGROUND OF THE INVENTION

In the breeding of animals, there are problems such as the frequent occurrence of diarrhea and other diseases. In general, there has been supplied to animals an antibiotic or a feed stuff containing an antibiotic to avoid those diseases. However, in such cases, it has been fairly difficult to prevent such diseases. Further, the application of a large quantity of the antibiotics to animals causes the remaining of the antibiotics in the body, eggs, milk and the like of animals. The remaining antibiotics bring harmful influences on people who take meats, eggs and milk containing the antibiotics to produce sometimes anti-drugs bacilli in the bodies of the people.

Now, we have found a safe pharmaceutical composition for animals which is based on natural materials and can prevent the frequent occurrence of diarrhea and other diseases of animals.

Therefore, it is an object of the present invention to provide a pharmaceutical composition for animals which is based on safe natural materials and which can step up the growth of the animals by the prevention of the frequent occurrence of diarrhea and other diseases of animals. A further object is to provide a pharmaceutical composition favored by animals. Another object is to provide a pharmaceutical composition for animals being safe for people who take meats, eggs and milks of the animals.

SUMMARY OF THE INVENTION

The present invention relates to a pharmaceutical composition for animals excluding humans comprising:

(A) at least one of a vinegar, a flesh of Japanese apricot (ume apricot), oranges and strawberries, and

(B) (i) saccharides or (ii) a combination of saccharides and an edible organic acid.

Further, the present invention relates to a pharmaceutical composition for animals excluding humans comprising:

(A) at least one of a vinegar, a flesh of Japanese apricot (ume apricot), oranges and strawberries,

(B) (i) saccharides or (ii) a combination of saccharides and an edible organic acid, and

(C) Lactobacillus bifidus.

Still further, the present invention relates to a method of supplying said pharmaceutical compositions to animals.

In addition, the present invention relates to a feed stuff for animals containing said pharmaceutical compositions.

DETAILED DESCRIPTION

The pharmaceutical composition of the present invention may be fed to animals excluding humans such as domestic animals, e.g. cattle, horse, pig, sheep, goat and the like; poultries, e.g. hen, cock, chicken, turkey, duck, guinea fowl, quail and the like; pets, e.g. dog, cat and the like; fishes, e.g. yellowtail, horse mackerel, flat fish, sea bream, red sea bream, eel, carp, rainbow trout, sweet fish, tilapia and the like.

In the present invention, both of a fermented vinegar and a synthesized vinegar may be used as the vinegar in component (A). A fermented vinegar, which includes rice vinegar, malt vinegar, alcohol vinegar, cider vinegar, wine vinegar or the like, is preferable. Both of liquid and solid vinegars may be used. A powdery vinegar is more preferable in the view point of easy handling and easy feeding to animals and the like.

The flesh of Japanese apricot used in said component (A) may be used in the smashed form or in the form of powder. Extractives from the flesh of Japanese apricot also may be used. The flesh of Japanese apricot may be one previously salted or dipped in alcohol. The raw flesh of Japanese apricot also may be used. However, when the raw flesh of Japanese apricot is used, it should be avoided that the raw flesh is contaminated with hydrocyanic acid contained in the stone of the raw Japanese apricot.

The oranges, tomatoes and strawberries may be used in the form of smashed form, powder, juice and the like. Further, a powdery rind of oranges may be used. As the oranges, there may be used lemons, satsuma mandarins, oranges, citron fruits and the like.

The vinegars, flesh of Japanese apricot, oranges, tomatoes and strawberries may be used alone or in admixture of two or more of them. They provide the pharmaceutical composition of the present invention with a good taste to make the animals take the composition readily. Especially, vinegars and the flesh of the Japanese apricot are preferable, and they may be used alone or in combination of them.

In the present invention, as the saccharides in said component (B), there may be used sugar, glucose, lactose, fruit sugar and the like, which may be used alone or in combination of them. Especially, sugar and glucose are preferable in view of the animal's favorite.

As edible organic acid in said component (B), any of animal-edible organic acids may be used. As examples of them, there may be used carboxylic acids such as citric acid, malic acid, fumaric acid, formic acid, succinic acid, tartaric acid, lactic acid and the like, which may be used alone or in combination of them. Among them, citric acid, fumaric acid and lactic aid are more preferable in the view points of the prevention of diarrhea and other diseases of animals and animal's favorite.

Some of those carboxylic acids are the same as carboxylic acids included in said vinegars, flesh of Japanese apricot, oranges, tomatoes and strawberries used as component (A) in the present invention. However, said "edible organic acid" used as component (B) in the present invention means a refined organic acid and is added into the feed composition of the present invention independently of said component (A). As said component (B), saccharides may be used alone or in admixture with the edible organic acid.

The occurrence of diseases of animals is more effectively prevented by the addition of Lactobacillus bifidus to the pharmaceutical composition comprising said components (A) and (B). Both of dried and undried Lactobacillus bifidus may be used.

In the present invention, a mix proportion of each component may vary depending on animal's kind, age, body weight and the like. In general, the pharmaceutical composition of the present invention comprises about 0.1-50% by weight of the component (A) and about 99.9-50% by weight of component (B). When mixtures of saccharides and the edible organic acid are used as the component (B) in the present invention, the pharmaceutical composition of the present invention comprises about 0.1-50% of at least one of the vinegar, flesh of Japanese apricot, oranges, tomatoes and strawberries, about 10-99.9% of the saccharides and about 1-60% of the edible organic acid based on the total weight of the pharmaceutical composition. When Lactobacillus bifidus is further added, in general, it is added in amounts of about 2.5.times.10.sup.6-8 cells per 100 g of a dry basis of the components (A) and (B).

The preferable embodiments of the present invention are as follows:

In the following embodiments, "%" is based on the total weight of the composition.

(1) A pharmaceutical composition comprising 1-25% of the vinegar, 1-20% of the flesh of Japanese apricot, 15-95.5% of saccharide and 2.5-40% of the edible organic acid.

(2) A pharmaceutical composition comprising 1-25% of the vinegar and 75-99% of saccharides.

(3) A pharmaceutical composition comprising 1-20% of the flesh of the Japanese apricot and 80-99% of saccharides. (4) A pharmaceutical composition comprising 1-25% of the vinegar, 1-20% of the flesh of Japanese apricot and 55-98% of saccharides.

(5) A pharmaceutical composition comprising 1-25% of the vinegar, 1-20% of the flesh of Japanese apricot, 15-95.5% of saccharide and 2.5-40% of the edible organic acid and 2.5.times.10.sup.8-10 cells of the Lactobacillus bifidus per 100 g on a dry basis of the vinegar, flesh of Japanese apricot, saccharides and edible organic acid.

(6) A pharmaceutical composition comprising 1-25% of the vinegar, 35-96.5% of saccharides and 2.5-40% of the edible organic acid and 2.5.times.10.sup.8-10 cells of the Lactobacillus bifidus per 100 g on a dry basis of the vinegar, saccharides and edible organic acid.

(7) A pharmaceutical composition comprising 1-20% of the flesh of Japanese apricot. 40-96.5% of saccharides and 2.5-40% of the edible organic acid and 2.5.times.10.sup.8-10 cells of the Lactobacillus bifidus per 100 g on a dry basis of the flesh of Japanese apricot, saccharides and edible organic acid.

Each component element is mixed according to any conventional methods.

The pharmaceutical composition for animals of the present invention may be solely fed to animals excluding humans. However, the pharmaceutical composition is more preferably fed to animals in the form of a solution or dispersion in water, or in admixture with a base feed stuff such as corns, wheat bran, wheat, wheat flour, soybean meal, milo, fish meal, fish powder, their mixture or the like. In such cases, about 0.1-10% of the pharmaceutical composition is generally added to water or the base feed stuff based on the weight of water or the feed stuff.

Further, the pharmaceutical composition may previously be mixed with the feed stuff.

In general, the pharmaceutical composition of the invention is fed to animals in the amount of about 0.01-10 g per 1 kg of the body weight of the animal. However, the amount may be varied.

Further, the pharmaceutical composition of the invention may be used in admixture with other therapeutic agents.

The pharmaceutical composition for animals of the invention can prevent more effectively the frequent occurrence of diarrhea, loose feces and other diseases than a composition consisting of saccharides and an edible organic acid. As the result, the pharmaceutical composition of the present invention can step up the growth of the animals.

The pharmaceutical composition of the present invention is based on only safe natural materials and thus is safe to animals and people who take meats, eggs, milk and the like of the animals, and animals take it favorably. The animals which take the pharmaceutical composition of the present invention provide us with good meats. When the pharmaceutical composition of the present invention is fed to cows, the production of milk increases, and the proportion of the milk fat in milk increases.

Further, the present pharmaceutical composition for animals can be fed to animals in admixture with other therapeutic agents.

The present invention described herein is illustrated by the following examples. These examples do not limit the present invention.

In the following examples, "Mean Mil Fat percentage" (called hereinafter "M.M.F. %"), "Proportion of Grown Animals" (called hereinafter "P.G.A."), "Increased Body Weight/animal.multidot.day" (called hereinafter "I.W./a.d.") and "Ingestion amount/animal.multidot.day" (called hereinafter "Ingestion") are calculated according to the following equations. ##EQU1##

In the following examples, a powdery rice vinegar (Mitsukansu Co.) was used as the vinegar, and a powdery flesh of Japanese apricot produced by Ikeda Touka Co. was used as the flesh of Japanese apricot, and powdery rind of lemon (Nankaikako Co.) was used as the lemon powder, and tomato powder produced by Spreader Co. was used as the tomato powder, and dried Lactobacillus bifidus (Oriental Koubo Co.) was used as the Lactobacillus bifidus.

EXAMPLES 1-94 AND COMPARATIVE EXAMPLES 1-38

Components shown in Table 1 below ere mixed in accordance with weight proportions shown in Table 1 to prepare pharmaceutical compositions for animals of the present Examples 1-94 and Comparative Examples 1-38.

Each pharmaceutical composition was diluted with water by way of a given magnification shown in Table 1, and then fed freely to animals instead of water. After a given test period, the total number of animals suffering from diarrhea and loose feces (called hereinafter "total D & L") was counted. The results are shown in Table 1 below.

TABLE 1-(i) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Results Powdery flesh Lactobacillus Kind Number Feed Total D&L/ Citric of Japanese bifidus of of periods Dilution total number Sugar acid Vinegar apricot (cells/100 g) animal animals (days) (times) of __________________________________________________________________________ animals Example 1 90 7.5 2.5 Pig (4 10 21 25 42/210 weeks old) 2 91.25 7.5 1.25 Pig (4 10 21 25 40/210 weeks old) 3 88.75 7.5 2.5 1.25 Pig (4 10 21 25 20/210 weeks old) 4 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Pig (4 10 21 25 30/210 weeks old) 5 91.25 7.5 1.25 2.5 .times. 10.sup.8 /100 g Pig (4 10 21 25 31/210 weeks old) 6-A 88.75 7.5 2.5 1.25 2.5 .times. 10.sup.8 /100 g Pig (4 10 21 25 15/210 weeks old) 6-B 92.5 7.5 Pig (4 10 21 25 41/210 weeks old) 6-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Pig (4 10 21 25 40/210 weeks old) Comparative Example 1 92.5 7.5 Pig (4 10 21 25 71/210 weeks old) Example 7 96.9 3 0.1 Pig (4 10 21 25 60/210 weeks old) 8 96.9 3 0.1 Pig (4 10 21 25 67/210 weeks old) 9 96.8 3 0.1 0.1 Pig (4 10 21 25 50/210 weeks old) 10 96.9 3 0.1 2.5 .times. 10.sup.6 /100 g Pig (4 10 21 25 52/210 weeks old) 11 96.9 3 0.1 2.5 .times. 10.sup.6 /100 g Pig (4 10 21 25 53/210 weeks old) 12-A 96.8 3 0.1 0.1 2.5 .times. 10.sup.6 /100 g Pig (4 10 21 25 45/210 weeks old) 12-B 97 3 2.5 .times. 10.sup.6 /100 g Pig (4 10 21 25 59/210 weeks old) 12-C 97 3 Pig (4 10 21 25 60/210 weeks old) Comparative Example 2 97 3 Pig (4 10 21 25 75/210 weeks old) Example 13 40 25 15 20 Pig (4 10 21 25 30/210 weeks old) 14 55 25 20 10 21 25 31/210 15 40 25 15 20 Pig (4 10 21 25 12/210 weeks old) 16 60 25 15 2.5 .times. 10.sup.10 /100 g Pig (4 10 21 25 22/210 weeks old) 17 55 25 20 2.5 .times. 10.sup.10 /100 g Pig (4 10 21 25 21/210 weeks old) 18-A 40 25 15 20 2.5 .times. 10.sup.10 /100 g Pig (4 10 21 25 8/210 weeks old) 18-B 75 25 Pig (4 10 21 25 29/210 weeks old) 18-C 75 5 20 2.5 .times. 10.sup.10 /100 g Pig (4 10 21 25 31/210 weeks old) Comparative Example 3 75 25 Pig (4 10 21 25 60/210 weeks old) Example 19 90 7.5 2.5 Calf (2 10 21 25 50/210 weeks old) 20 91.25 7.5 1.25 Calf (2 10 21 25 49/210 weeks old) 21 88.75 7.5 2.5 1.25 Calf (2 10 21 25 25/210 weeks old) 22-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Calf (2 10 21 25 32/210 weeks old) 22-B 92.5 7.5 2.5 .times. 10.sup.8 /100 g Calf (2 10 21 25 48/210 weeks old) 22-C 92.5 7.5 Calf (2 10 21 25 50/210 weeks old) Comparative Example 4 92.5 7.5 Calf (2 10 21 25 108/210 weeks old) Example 23 96.9 3 0.1 Calf (2 10 21 25 60/210 weeks old) 24 96.9 3 0.1 Calf (2 10 21 25 56/210 weeks old) 25 96.8 3 0.1 0.1 Calf (2 10 21 25 34/210 weeks old) 26-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Calf (2 10 21 25 40/210 weeks old) 26-B 97 3 Calf (2 10 21 25 57/210 weeks old) 26-C 97 3 2.5 .times. 10.sup.8 /100 g Calf (2 10 21 25 59/210 weeks old) Comparative Example 5 97 3 Calf (2 10 21 25 98/210 weeks old) Example 27 40 25 15 20 Calf (2 10 21 100 60/210 weeks old) 28-A 60 25 15 2.5 .times. 10.sup.10 /100 g Calf (2 10 21 100 44/210 weeks old) 28-B 75 25 2.5 .times. 10.sup.10 /100 g Calf (2 10 21 100 61/210 weeks old) 28-C 75 5 20 Calf (2 10 21 100 58/210 weeks old) Comparative Example 6 75 25 Calf (2 10 21 100 101/210 weeks old) Example 29 90 7.5 2.5 Lamb (4 10 28 25 22/280 weeks old) 30 91.25 7.5 1.25 Lamb (4 10 28 25 20/280 weeks old) 31 88.75 7.5 2.5 1.25 Lamb (4 10 28 25 10/280 weeks old) 32-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Lamb (4 10 28 25 15/280 weeks old) 32-B 92.5 7.5 Lamb (4 10 28 25 23/280 weeks old) 32-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Lamb (4 10 28 25 20/280 weeks old) Comparative Example 7 92.5 7.5 Lamb (4 10 28 25 62/280 weeks old) Example 33 96.9 3 0.1 Lamb (4 10 28 25 32/280 weeks old) 34 96.9 3 0.1 Lamb (4 10 28 25 30/280

weeks old) 35 96.8 3 0.1 0.1 Lamb (4 10 28 25 20/280 weeks old) 36-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Lamb (4 10 28 25 29/280 weeks old) 36-B 97 3 2.5 .times. 10.sup.8 /100 g Lamb (4 10 28 25 31/280 weeks old) 36-C 97 3 Lamb (4 10 28 25 33/280 weeks old) Comparative Example 8 97.0 3 Lamb (4 10 28 25 60/280 weeks old) Example 37 40 25 15 20 Lamb (4 10 28 100 40/280 weeks old) 38-A 60 25 15 2.5 .times. 10.sup.10 /100 g Lamb (4 10 28 100 36/280 weeks old) 38-B 75 25 Lamb (4 10 28 100 38/280 weeks old) 38-C 75 5 20 2.5 .times. 10.sup.10 /100 g Lamb (4 10 28 100 40/280 weeks old) Comparative Example 9 75 25 Lamb (4 10 28 100 61/280 weeks old) Example 39 90 7.5 2.5 Chicken (3 200 35 25 423/7000 weeks old) 40 91.25 7.5 1.25 Chicken (3 200 35 25 403/7000 weeks old) 41 88.75 7.5 2.5 1.25 Chicken (3 200 35 25 198/7000 weeks old) 42-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Chicken (3 200 35 25 309/7000 weeks old) 42-B 92.5 7.5 2.5 .times. 10.sup.8 /100 g Chicken (3 200 35 25 403/7000 weeks old) 42-C 92.5 7.5 Chicken (3 200 35 25 394/7000 weeks old) Comparative Example 10 92.5 7.5 Chicken (3 200 35 25 1066/7000 weeks old) Example 43 96.9 3 0.1 Chicken (3 200 35 25 603/7000 weeks old) 44 96.9 3 0.1 Chicken (3 200 35 25 600/7000 weeks old) 45 96.8 3 0.1 0.1 Chicken (3 200 35 25 503/7000 weeks old) 46-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Chicken (3 200 35 25 621/7000 weeks old) 46-B 97 3 Chicken (3 200 35 25 634/7000 weeks old) 46-C 97 3 2.5 .times. 10.sup.8 /100 g Chicken (3 200 35 25 611/7000 weeks old) Comparative Example 11 97.0 3 Chicken (3 200 35 25 1050/7000 weeks old) Example 47 40 25 15 20 Chicken (3 200 35 100 611/7000 weeks old) 48-A 60 25 15 2.5 .times. 10.sup.10 /100 g Chicken (3 200 35 100 601/7000 weeks old) 48-B 75 25 2.5 .times. 10.sup.10 /100 g Chicken (3 200 35 100 641/7000 weeks old) 48-C 75 24 1 Chicken (3 200 35 100 636/7000 weeks old) Comparative Example 12 75 25 Chicken (3 200 35 100 1023/7000 weeks old) Example 49 90 7.5 2.5 Dog (65 20 35 25 40/700 days old) 50 91.25 7.5 1.25 Dog (65 20 35 25 45/700 days old) 51 88.75 7.5 2.5 1.25 Dog (65 20 35 25 25/700 days old) 52-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Dog (65 20 35 25 36/700 days old) 52-B 92.5 7.5 Dog (65 20 35 25 41/700 days old) 52-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Dog (65 20 35 25 50/700 days old) Comparative Example 13 92.5 7.5 Dog (65 20 35 25 141/700 days old) Example 53 96.9 3 0.1 Dog (65 20 35 25 62/700 days old) 54 96.9 3 0.1 Dog (65 20 35 25 64/700 days old) 55 96.8 3 0.1 0.1 Dog (65 20 35 25 50/700 days old) 56-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Dog (65 20 35 25 50/700 days old) 56-B 97 3 2.5 .times. 10.sup.8 /100 g Dog (65 20 35 25 66/700 days old) 56-C 97 3 Dog (65 20 35 25 58/700 days old) Comparative Example 14 97 3 Dog (65 20 35 25 120/700 days old) Example 57 40 25 15 20 Dog (65 20 35 100 70/700 days old) 58-A 60 25 15 2.5 .times. 10.sup.10 /100 g Dog (65 20 35 100 72/700 days old) 58-B 75 5 20 Dog (65 20 35 100 60/700 days old) 58-C 75 24 1 2.5 .times. 10.sup.10 /100 g Dog (65 20 35 100 65/700 days old) Comparative Example 15 75 25 Dog (65 20 35 100 112/700 days old) Example 59 10 50 30 10 Dog (65 20 35 100 62/700 days old) 60 10 50 15 25 Dog (65 20 35 100 59/700 days old) 61-A 88 1 1 10 Dog (65 20 35 100 56/700 days old) 61-B 99 1 Dog (65 20 35 100 60/700 days old) 61-C 99 1 Dog (65 20 35 100 63/700 days old) Comparative Example 16 10 90 Dog (65 20 35 100 116/700 days old) 17 50 50 Dog (65 20 35 100 109/700 days old) 18 99 1 Dog (65 20 35 100 113/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times)

TABLE 1-(ii) __________________________________________________________________________ Mix proportions of each component in pharma- ceutical composition for animals (wt. %) Results Powdery Kind Number Feed Total D&L/ Lactic rind of of of periods Dilution (*) total number Glucose acid Vinegar lemon animal animals (days) (times) of animals __________________________________________________________________________ Example 62 10 50 30 10 Dog (65 20 35 100 60/700 days old) 63 10 50 15 25 Dog (65 20 35 100 58/700 days old) 64 88 1 1 10 Dog (65 20 35 100 61/700 days old) 65 98.9 1 0.1 Dog (65 20 35 100 57/700 days old) 66-A 88.75 7.5 2.5 1.25 Dog (65 20 35 100 40/700 days old) 66-B 92.5 2.5 5 Dog (65 20 35 100 61/700 days old) 66-C* 92.5 Dog (65 20 35 100 64/700 days old) Comparative Example 19 10 90 Dog (65 20 35 100 100/700 days old) 20 50 50 Dog (65 20 35 100 110/700 days old) 21 99 1 Dog (65 20 35 100 108/700 days old) 22 92.5 7.5 Dog (65 20 35 100 110/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times) *In addition to glucose, 2.5 wt. % of powdery flesh of Japanese apricot and 50 wt. % of powdery tomato are mixed.

TABLE 1-(iii) __________________________________________________________________________ Mix proportions of each component in pharma- Results ceutical composition for animals (wt. %) Kind Number Feed Total D&L/ Fumaric Powdery of of periods Dilution (*) total number Glucose acid Vinegar tomato animal animals (days) (times) of animals __________________________________________________________________________ Example 67 10 50 30 10 Dog (65 20 35 100 61/700 days old) 68 10 50 15 25 Dog (65 20 35 100 63/700 days old) 69 88 1 1 10 Dog (65 20 35 100 60/700 days old) 70 98.9 1 0.1 Dog (65 20 35 100 58/700 days old) 71-A 88.75 7.5 2.5 1.25 Dog (65 20 35 100 38/700 days old) 71-B*.sup.2 99 Dog (65 20 35 100 60/700 days old) 71-C 99 1 Dog (65 20 35 100 59/700 days old) Comparative Example 23 10 90 Dog (65 20 35 100 101/700 days old) 24 50 50 Dog (65 20 35 100 105/700 days old) 25 99 1 Dog (65 20 35 100 109/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times) *.sup.2 In addition to 99 wt. % of glucose, 1 wt. % of powdery rind of lemon is mixed.

TABLE 1-(iv) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Results Powdery flesh Lactobacillus Kind Number Feed Total D&L/ Citric of Japanese bifidus of of periods Dilution total number Sugar acid Vinegar apricot (cells/100 g) animal animals (days) (times) of __________________________________________________________________________ animals Example 72 90 7.5 2.5 Cat (60 20 35 25 21/700 days old) 73 91.25 7.5 1.25 Cat (60 20 35 25 22/700 days old) 74 88.75 7.5 2.5 1.25 Cat (60 20 35 25 14/700 days old) 75-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Cat (60 20 35 25 20/700 days old) 75-B 92.5 7.5 Cat (60 20 35 25 23/700 days old) 75-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Cat (60 20 35 25 25/700 days old) Comparative Example 26 92.5 7.5 Cat (60 20 21 25 85/700 days old) Example 76 96.9 3 0.1 Cat (60 20 35 25 50/700 days old) 77 96.9 3 0.1 Cat (60 20 35 25 51/700 days old) 78 96.8 3 0.1 0.1 Cat (60 20 35 25 40/700 days old) 79-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Cat (60 20 35 25 49/700 days old) 79-B 97 3 2.5 .times. 10.sup.8 /100 g Cat (60 20 35 25 52/700 days old) 79-C 97 3 Cat (60 20 35 25 54/700 days old) Comparative Example 27 97 3 Cat (60 20 35 25 81/700 days old) Example 80 40 25 15 20 Cat (60 20 35 100 47/700 days old) 81-A 60 25 15 2.5 .times. 10.sup.10 /100 g Cat (60 20 35 100 44/700 days old) 81-B 75 5 20 Cat (60 20 35 100 46/700 days old) 81-C*.sup.3 75 2.5 .times. 10.sup.10 /100 g Cat (60 20 35 100 47/700 days old) Comparative Example 28 75 25 Cat (60 20 35 100 76/700 days old) Example 82 10 50 30 10 Cat (60 20 35 100 50/700 days old) 83 10 50 15 25 Cat (60 20 35 100 51/700 days old) 84-A 88 1 1 10 Cat (60 20 35 100 48/700 days old) 84-B 99 1 Cat (60 20 35 100 49/700 days old) 84-C 99 1 Cat (60 20 35 100 52/700 days old) Comparative Example 29 10 90 Cat (60 20 35 100 81/700 days old) 30 50 50 Cat (60 20 35 100 85/700 days old) 31 99 1 Cat (60 20 35 100 80/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times) *.sup.3 In addition to 75 wt. % of sugar and 2.5 .times. 10.sup.10 /100 g of Lactobacillus bifidus, 5 wt. % of powdery rind of lemon and 20 wt. % o powdery tomato are mixed.

TABLE 1-(v) __________________________________________________________________________ Mix proportions of each component in pharma- ceutical composition for animals (wt. %) Results Powdery Kind Number Feed Total D&L/ Lactic rind of of of periods Dilution (*) total number Glucose acid Vinegar lemon animal animals (days) (times) of animals __________________________________________________________________________ Example 85 10 50 30 10 Cat (60 20 35 100 48/700 days old) 86 10 50 15 25 Cat (60 20 35 100 47/700 days old) 87 88 1 1 10 Cat (60 20 35 100 49/700 days old) 88 98.9 1 0.1 Cat (60 20 35 100 48/700 days old) 89-A 88.75 7.5 2.5 1.25 Cat (60 20 35 100 25/700 days old) 89-B 99 1 Cat (60 20 35 100 50/700 days old) 89-C*.sup.4 99 1 Cat (60 20 35 100 52/700 days old) 89-D*.sup.5 92.5 5 Cat (60 20 35 100 48/700 days old) 89-E*.sup.6 92.5 5 Cat (60 20 35 100 49/700 days old) Comparative Example 32 10 90 Cat (60 20 35 100 89/700 days old) 33 50 50 Cat (60 20 35 100 90/700 days old) 34 99 1 Cat (60 20 35 100 88/700 days old) 35 92.5 7.5 Cat (60 20 35 100 89/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times) *.sup.4 In addition to 99 wt. % of glucose, 1 wt. % of powdery flesh of Japanese apricot is mixed. *.sup.5 In addition to 92.5 wt. % of glucose and 5 wt. % of vinegar, 2.5 wt. % of powdery flesh of Japanese apricot is mixed. *.sup.6 In addition to 92.5 wt. % of glucose and 5 wt. % of powdery rind of lemon, 2.5 wt. % of powdery tomato is mixed.

TABLE 1-(vi) __________________________________________________________________________ Mix proportions of each component in pharma- Results ceutical composition for animals (wt. %) Kind Number Feed Total D&L/ Fumaric Powdery of of periods Dilution (*) total number Glucose acid Vinegar tomato animal animals (days) (times) of animals __________________________________________________________________________ Example 90 10 50 30 10 Cat (60 20 35 100 43/700 days old) 91 10 50 15 25 Cat (60 20 35 100 48/700 days old) 92 88 1 1 10 Cat (60 20 35 100 49/700 days old) 93 98.9 1 0.1 Cat (60 20 35 100 47/700 days old) 94-A 88.75 7.5 2.5 1.25 Cat (60 20 35 100 28/700 days old) 94-B*.sup.7 99 Cat (60 20 35 100 51/700 days old) 94-C 99 1 Cat (60 20 35 100 53/700 days old) Comparative Example 36 10 90 Cat (60 20 35 100 90/700 days old) 37 50 50 Cat (60 20 35 100 87/700 days old) 38 99 1 Cat (60 20 35 100 88/700 days old) __________________________________________________________________________ (*) Water dilution magnification (times) *.sup.7 In addition to 99 wt. % of glucose, 1 wt. % of powdery rind of lemon is mixed.

EXAMPLES 95-114 AND COMPARATIVE EXAMPLES 39-44

Components shown in Table 2 below were mixed in accordance with weight proportions shown in Table 2 to prepare pharmaceutical compositions for animals of the present Examples 95-114 and Comparative Examples 39-44.

Each of the pharmaceutical composition for animals was added to Base Feed Stuff A or B shown below at a given weight proportion shown in Table 2 to prepare test feed stuffs. Each of the test feed stuffs was fed to animals freely. "I.W./a.d." and "Ingestion" were determined. The results are shown in Table 2 below.

______________________________________ Composition of Base Feed Stuff A ______________________________________ Corn 450 weight parts Milo 310 weight parts Soybean meal 146 weight parts Rapeseed meal 30 weight parts Fish powder 15 weight parts Meat born meal 10 weight parts Yellow grease 18 weight parts Calcium carbonate 5 weight parts Calcium phosphate 8 weight parts Salt 3 weight parts Premix vitamins & minerals 5 weight parts ______________________________________

______________________________________ Composition of Base Feed Stuff B ______________________________________ Corn 300 weight parts Milo 135 weight parts Wheat bran 70 weight parts Soybean meal 246 weight parts Wheat flour 180 weight parts Yellow grease 7 weight parts Molasses 30 weight parts Calcium carbonate 14.5 weight parts Calcium triphosphate 11 weight parts Salt 5 weight parts Vitamins and Minerals 1.5 weight parts ______________________________________

TABLE 2 __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Num- Powdery flesh Lactobacillus Kind ber Feed In- Citric Vine- of Japanese bifidus of of periods P.F.B.** I.W./ gestion Sugar acid gar apricot (cells/100 g) animal animals (days) K.B.F.S.* (wt. %) a.d. (g) __________________________________________________________________________ Example 95 90 7.5 2.5 Pig (4 20 14 A 2 471 500 weeks old) 96 91.25 7.5 1.25 Pig (4 20 14 A 2 464 490 weeks old) 97 88.75 7.5 2.5 1.25 Pig (4 20 14 A 2 468 510 weeks old) 98-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Pig (4 20 14 A 2 479 508 weeks old) 98-B 92.5 7.5 2.5 .times. 10.sup.8 /100 g Pig (4 20 14 A 2 469 511 weeks old) 98-C 92.5 7.5 Pig (4 20 14 A 2 465 506 weeks old) Compar- ative Example 39 92.5 7.5 Pig (4 20 14 A 2 386 435 weeks old) Example 99 96.9 3 0.1 Pig (4 20 14 A 2 464 491 weeks old) 100 96.9 3 0.1 Pig (4 20 14 A 2 450 481 weeks old) 101 96.8 3 0.1 0.1 Pig (4 20 14 A 2 460 498 weeks old) 102-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Pig (4 20 14 A 2 455 490 weeks old) 102-B 97 3 Pig (4 20 14 A 2 450 480 weeks old) 102-C 97 3 2.5 .times. 10.sup.8 /100 g Pig (4 20 14 A 2 448 478 weeks old) Compar- ative Example 40 97 3 Pig (4 20 14 A 2 388 440 weeks old) Example 103 40 25 15 20 Pig (4 20 14 A 2 453 486 weeks old) 104-A 60 25 15 2.5 .times. 10.sup.10 /100 g Pig (4 20 14 A 2 451 483 weeks old) 104-B 75 25 2.5 .times. 10.sup.10 /100 g Pig (4 20 14 A 2 448 478 weeks old) 104-C 75 5 20 Pig (4 20 14 A 2 446 479 weeks old) Compar- ative Example 41 75 25 Pig (4 20 14 A 2 398 456 weeks old) Example 105 90 7.5 2.5 Calf (3 20 21 B 4 1029 1780 weeks old) 106 91.25 7.5 1.25 Calf (3 20 21 B 4 1038 1769 weeks old) 107 88.75 7.5 2.5 1.25 Calf (3 20 21 B 4 1043 1779 weeks old) 108-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Calf (3 20 21 B 4 1029 1768 weeks old) 108-B 92.5 7.5 Calf (3 20 21 B 4 1021 1760 weeks old) 108-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Calf (3 20 21 B 4 1016 1758 weeks old) Compar- ative Example 42 92.5 7.5 Calf (3 20 21 B 4 880 1680 weeks old) Example 109 96.9 3 0.1 Calf (3 20 21 B 4 991 1769 weeks old) 110 96.9 3 0.1 Calf (3 20 21 B 4 986 1756 weeks old) 111 96.8 3 0.1 0.1 Calf (3 20 21 B 4 998 1768 weeks old) 112-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Calf (3 20 21 B 4 976 1750 weeks old) 112-B 97 3 2.5 .times. 10.sup.8 /100 g Calf (3 20 21 B 4 970 1740 weeks old) 112-C 97 3 Calf (3 20 21 B 4 965 1743 weeks old) Compar- ative Example 43 97 3 Calf (3 20 21 B 4 898 1698 weeks old) Example 113 40 25 15 20 Calf (3 20 21 B 4 976 1763 weeks old) 114-A 60 25 15 2.5 .times. 10.sup.10 /100 g Calf (3 20 21 B 4 981 1745 weeks old) 114-B 75 25 Calf (3 20 21 B 4 970 1748 weeks old) 114-C 75 5 20 2.5 .times. 10.sup.10 /100 g Calf (3 20 21 B 4 972 1741 weeks old) Compar- ative Example 44 75 25 Calf (3 20 21 B 4 887 1683 weeks old)

__________________________________________________________________________ *Kind of Base Feed Stuff **Proportion of the pharmaceutical composition added to Base Feed Stuff (wt. %)

EXAMPLES 115-157 AND COMPARATIVE EXAMPLES 45-63

Components shown in Table 3 below were mixed in accordance with weight proportions shown in Table 3 to prepare pharmaceutical compositions for animals of the present Examples 115-157 and Comparative Examples 45-63.

Each of the pharmaceutical composition for animals was added to Base Feed Stuff C shown below at a given weight proportion shown in Table 3 to prepare test feed stuffs. Each of the test feed stuff was fed to animals freely. The results are shown in Table 3 below.

______________________________________ Composition of Base Feed Stuff C ______________________________________ Corn 400 weight parts Milo 230 weight parts Soybean meal 270 weight parts Fish powder (65%) 50 weight parts Yellow grease 25 weight parts Calcium carbonate 5 weight parts Calcium orthophosphate 12 weight parts Salt 1.5 weight parts Premix vitamins & minerals 6.5 weight parts ______________________________________

TABLE 3-(i) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Num- Ci- Powdery flesh Lactobacillus Kind ber Feed tric Vine- of Japanese bifidus of of periods P.F.B.** P.G.A. Sugar acid gar apricot (cells/100 g) animal animals (days) K.B.F.S.* (wt. (%) __________________________________________________________________________ Example 115 90 7.5 2.5 Chicken (15 5200 45 C 1 99.6 days old) 116 91.25 7.5 1.25 Chicken (15 5200 45 C 1 99.6 days old) 117 88.75 7.5 2.5 1.25 Chicken (15 5200 45 C 1 99.5 days old) 118-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Chicken (15 5200 45 C 1 99.4 days old) 118-B 92.5 7.5 2.5 .times. 10.sup. 8 /100 g Chicken (15 5200 45 C 1 99.2 days old) 118-C 92.5 7.5 Chicken (15 5200 45 C 1 99.1 days old) Comparative Example 45 92.5 7.5 Chicken (15 5200 45 C 1 95.3 days old) Example 119 96.9 3 0.1 Chicken (15 5200 45 C 1 98.7 days old) 120 96.9 3 0.1 Chicken (15 5200 45 C 1 98.8 days old) 121 96.8 3 0.1 0.1 Chicken (15 5200 45 C 1 99.1 days old) 122-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Chicken (15 5200 45 C 1 98.9 days old) 122-B 97 3 Chicken (15 5200 45 C 1 98.7 days old) 122-C 97 3 2.5 .times. 10.sup.8 /100 g Chicken (15 5200 45 C 1 98.8 days old) Comparative Example 46 97 3 Chicken (15 5200 45 C 1 96.9 days old) Example 123 40 25 15 20 Chicken (15 5200 45 C 1 98.9 days old) 124-A 60 25 15 2.5 .times. 10.sup.10 /100 g Chicken (15 5200 45 C 1 98.8 days old) 124-B 75 25 2.5 .times. 10.sup.10 /100 g Chicken (15 5200 45 C 1 98.8 days old) 124-C 75 5 20 Chicken (15 5200 45 C 1 98.8 days old) Comparative Example 47 75 25 Chicken (15 5200 45 C 1 96.8 days old) Example 125 10 50 30 10 Chicken (15 2200 45 C 1 98.9 days old) 126 10 50 15 25 Chicken (15 2200 45 C 1 98.8 days old) 127-A 88 1 1 10 Chicken (15 2200 45 C 1 98.7 days old) 127-B 99 1 Chicken (15 2200 45 C 1 98.7 days old) 127-C 99 1 Chicken (15 2200 45 C 1 98.6 days old) Comparative Example 48 10 90 Chicken (15 2200 45 C 1 96.7 days old) 49 50 50 Chicken (15 2200 45 C 1 96.6 days old) 50 99 1 Chicken (15 2200 45 C 1 96.9 days old) __________________________________________________________________________ *Kind of Base Feed Stuff **Proportion of the pharmaceutical composition added to Base Feed Stuff (wt. %)

TABLE 3-(ii) __________________________________________________________________________ Mix proportions of each component in pharma- Num- ceutical composition for animals (wt. %) Kind ber Feed Lactic rind of of of periods P.F.B.** Glucose acid Vinegar lemon animal animals (days) K.B.F.S.* (wt. P.G.A. __________________________________________________________________________ (%) Example 128 10 50 30 10 Chicken (15 2200 45 C 1 98.7 days old) 129 10 50 15 25 Chicken (15 2200 45 C 1 98.9 days old) 130 88 1 1 10 Chicken (15 2200 45 C 1 98.6 days old) 131 98.9 1 0.1 Chicken (15 2200 45 C 1 98.8 days old) 132-A 88.75 7.5 2.5 1.25 Chicken (15 2200 45 C 1 99.4 days old) 132-B 99 1 Chicken (15 2200 45 C 1 98.7 days old) 132-C*.sup.8 99 Chicken (15 2200 45 C 1 98.6 days old) 132-D 92.5 Chicken (15 2200 45 C 1 98.7 days old) 132-E*.sup.9 92.5 Chicken (15 2200 45 C 1 98.8 days old) Comparative Example 51 10 90 Chicken (15 2200 45 C 1 96.8 days old) 52 50 50 Chicken (15 2200 45 C 1 96.7 days old) 53 99 1 Chicken (15 2200 45 C 1 96.9 days old) 54 92.5 7.5 Chicken (15 2200 45 C 1 97.0 days old) __________________________________________________________________________ *Kind of Base Feed Stuff **Proportion of the pharmaceutical composition added to Base Feed Stuff (wt. %) *.sup.8 In addition to 99 wt. % of glucose, 1 wt. % of powdery tomato is mixed. *.sup.9 In addition to 92.5 wt. % of glucose, 7.5 wt. % of powdery tomato is mixed.

TABLE 3-(iii) __________________________________________________________________________ Mix proportions of each component in pharma- ceutical composition for animals (wt. %) Kind Number Feed Fumaric Powdery of of periods P.F.B.** Glucose acid Vinegar tomato animal animals (days) K.B.F.S.* (wt P.G.A. __________________________________________________________________________ (%) Example 133 10 50 30 10 Chicken (15 2200 45 C 1 98.8 days old) 134 10 50 15 25 Chicken (15 2200 45 C 1 98.9 days old) 135 88 1 1 10 Chicken (15 2200 45 C 1 98.7 days old) 136 98.9 1 0.1 Chicken (15 2200 45 C 1 98.8 days old) 137 88.75 7.5 2.5 1.25 Chicken (15 2200 45 C 1 99.3 days old) Comparative Example 55 10 90 Chicken (15 2200 45 C 1 96.9 days old) 56 50 50 Chicken (15 2200 45 C 1 96.8 days old) 57 99 1 Chicken (15 2200 45 C 1 96.9 days old) __________________________________________________________________________ *Kind of Base Feed Stuff **Proportion of the pharmaceutical composition added to Base Feed Stuff (wt. %)

TABLE 3-(iv) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Num- Powdery flesh Lactobacillus Kind ber Feed Citric of Japanese bifidus of of periods P.F.B.** P.G.A. Sugar acid Vinegar apricot (cells/100 g) animal animals (days) K.B.F.S.* (wt. (%) __________________________________________________________________________ Example 138 90 7.5 2.5 Quail (20 500 40 C 1 98.4 days old) 139 91.25 7.5 1.25 Quail (20 500 40 C 1 98.6 days old) 140 88.75 7.5 2.5 1.25 Quail (20 500 40 C 1 98.8 days old) 141-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Quail (20 500 40 C 1 98.6 days old) 141-B 92.5 7.5 2.5 .times. 10.sup. 8 /100 g Quail (20 500 40 C 1 98.4 days old) 141-C 92.5 7.5 Quail (20 500 40 C 1 98.2 days old) Comparative Example 58 92.5 7.5 Quail (20 500 40 C 1 93.8 days old) Example 142 96.9 3 0.1 Quail (20 500 40 C 1 97.4 days old) 143 96.9 3 0.1 Quail (20 500 40 C 1 97.6 days old) 144 96.8 3 0.1 0.1 Quail (20 500 40 C 1 98.0 days old) 145-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Quail (20 500 40 C 1 97.6 days old) 145-B 97 3 Quail (20 500 40 C 1 97.2 days old) 145-C 97 3 2.5 .times. 10.sup.8 /100 g Quail (20 500 40 C 1 97.4 days old) Comparative Example 59 97 3 Quail (20 500 40 C 1 95.4 days old) Example 146 40 25 15 20 Quail (20 500 40 C 1 97.4 days old) 147-A 60 25 15 2.5 .times. 10.sup.10 /100 g Quail (20 500 40 C 1 97.6 days old) 147-B 75 25 2.5 .times. 10.sup.10 /100 g Quail (20 500 40 C 1 97.4 days old) 147-C 75 5 20 Quail (20 500 40 C 1 97.2 days old) Comparative Example 60 75 25 Quail (20 500 40 C 1 95.2 days old) Example 148 90 7.5 2.5 Turkey 500 40 C 1 98.8 (20 days old) 149 91.25 7.5 1.25 Turkey 500 40 C 1 99.0 (20 days old) 150 88.75 7.5 2.5 1.25 Turkey 500 40 C 1 98.8 (20 days old) 151-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Turkey 500 40 C 1 99.0 (20 days old) 151-B 92.5 7.5 Turkey 500 40 C 1 98.6 (20 days old) 151-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Turkey 500 40 C 1 98.8 (20 days old) Comparative Example 61 92.5 7.5 Turkey 500 40 C 1 89.4 (20 days old) Example 152 96.9 3 0.1 Turkey 500 40 C 1 97.4 (20 days old) 153 96.9 3 0.1 Turkey 500 40 C 1 97.6 (20 days old) 154 96.8 3 0.1 0.1 Turkey 500 40 C 1 97.4 (20 days old) 155-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Turkey 500 40 C 1 97.6 (20 days old) 155-B 97 3 2.5 .times. 10.sup.8 /100 g Turkey 500 40 C 1 97.2 (20 days old) 155-C 97 3 Turkey 500 40 C 1 97.0 (20 days old) Comparative Example 62 97 3 Turkey 500 40 C 1 90.6 (20 days old) Example 156 40 25 15 20 Turkey 500 40 C 1 97.8 (20 days old) 157-A 60 25 15 2.5 .times. 10.sup.10 /100 Turkey 500 40 C 1 98.0 (20 days old) 157-B 75 25 Turkey 500 40 C 1 97.6 (20 days old) 157-C 75 5 20 2.5 .times. 10.sup.10 /100 g Turkey 500 40 C 1 97.4 (20 days old) Comparative Example 63 75 25 Turkey 500 40 C 1 90.2 (20 days old) __________________________________________________________________________ *Kind of Base Feed Stuff **Proportion of the pharmaceutical composition added to Base Feed Stuff (wt. %)

EXAMPLES 158-220 AND COMPARATIVE EXAMPLES 64-82

Components shown in Table 4 below were mixed in accordance with weight proportions shown in Table 4 to prepare pharmaceutical compositions for animals of the present Examples 158-220 and Comparative Examples 64-82. 1 g of each of the pharmaceutical compositions for animals was added to 100 g of Base Feed Stuff D, E or F shown below to prepare test feed stuffs. Each of the test feed stuffs was fed to animals freely. "Growth %" is shown in Table 4 below.

______________________________________ Composition of Base Feed Stuff D ______________________________________ Fish powder 69.4 weight parts Beer yeast 2.0 weight parts .alpha.-Starch 23.0 weight parts Vitamins 3.6 weight parts Minerals 2.0 weight parts ______________________________________

______________________________________ Composition of Base Feed Stuff E ______________________________________ Fish powder 65.0 weight parts Cod-liver oil 5.0 weight parts Wheat flour 17.4 weight parts Vitamins 4.0 weight parts Minerals 5.0 weight parts Carboxy methylcellulose (CMC) 5.0 weight parts ______________________________________

______________________________________ Composition of Base Feed Stuff F ______________________________________ Fish powder 74.4 weight parts Corn starch 6.0 weight parts Cod-liver oil 10.0 weight parts Vitamins 3.6 weight parts Minerals 1.0 weight parts CMC 5.0 weight parts ______________________________________

TABLE 4-(i) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Cit- Powdery flesh Lactobacillus Kind Number Feed Feed per ric of Japanese bifidus of of periods day Sugar acid Vinegar apricot (cells/100 g) fish fishes (days) K.B.F.S.* (times) P.G.A. __________________________________________________________________________ Example 158 90 7.5 2.5 Eel 500 70 D 1 97.6 (30 g/fish) 159 91.25 7.5 1.25 Eel 500 70 D 1 97.8 (30 g/fish) 160 88.75 7.5 2.5 1.25 Eel 500 70 D 1 97.8 (30 g/fish) 161-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Eel 500 70 D 1 97.8 (30 g/fish) 161-B 92.5 7.5 2.5 .times. 10.sup.8 /100 g Eel 500 70 D 1 97.4 (30 g/fish) 161-C 92.5 7.5 Eel 500 70 D 1 97.6 (30 g/fish) Comparative Example 64 92.5 7.5 Eel 500 70 D 1 82.0 (30 g/fish) Example 162 96.9 3 0.1 Eel 500 70 D 1 95.0 (30 g/fish) 163 96.9 3 0.1 Eel 500 70 D 1 95.4 (30 g/fish) 164 96.8 3 0.1 0.1 Eel 500 70 D 1 95.4 (30 g/fish) 165-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Eel 500 70 D 1 95.8 (30 g/fish) 165-B 97 3 Eel 500 70 D 1 95.0 (30 g/fish) 165-C 97 3 2.5 .times. 10.sup.8 /100 g Eel 500 70 D 1 95.2 (30 g/fish) Comparative Example 65 97 3 Eel 500 70 D 1 83.6 (30 g/fish) Example 166 40 25 15 20 Eel 500 70 D 1 96.8 (30 g/fish) 167-A 60 25 15 2.5 .times. 10.sup.10 /100 g Eel 500 70 D 1 96.8 (30 g/fish) 167-B 75 25 2.5 .times. 10.sup.10 /100 g Eel 500 70 D 1 96.2 (30 g/fish) 167-C 75 5 20 Eel 500 70 D 1 96.4 (30 g/fish) Comparative Example 66 75 25 Eel 500 70 D 1 83.2 (30 g/fish) Example 168 10 50 30 10 Eel 500 70 D 1 94.0 (30 g/fish) 169 10 50 15 25 Eel 500 70 D 1 93.6 (30 g/fish) 170-A 88 1 1 10 Eel 500 70 D 1 93.8 (30 g/fish) 170-B 99 1 Eel 500 70 D 1 93.0 (30 g/fish) 170-C 99 1 Eel 500 70 D 1 93.2 (30 g/fish) Comparative Example 67 10 90 Eel 500 70 D 1 88.6 (30 g/fish) 68 50 50 Eel 500 70 D 1 87.8 (30 g/fish) 69 99 1 Eel 500 70 D 1 88.4 (30 g/fish) __________________________________________________________________________ *Kind of Base Feed Stuff

TABLE 4-(ii) __________________________________________________________________________ Mix proportions of each component in pharma- ceutical composition for animals (wt. %) Powdery Kind Number Feed Feed per Lactic rind of of of periods day Glucose acid Vinegar lemon fish fishes (days) K.B.F.S.* (times) P.G.A. __________________________________________________________________________ (%) Example 171 10 50 30 10 Eel 500 70 D 1 94.2 (30 g/fish) 172 10 50 15 25 Eel 500 70 D 1 96.2 (30 g/fish) 173 88 1 1 10 Eel 500 70 D 1 95.6 (30 g/fish) 174 98.9 1 0.1 Eel 500 70 D 1 93.8 (30 g/fish) 175-A 88.75 7.5 2.5 1.25 Eel 500 70 D 1 97.6 (30 g/fish) 175-B 99 1 Eel 500 70 D 1 93.8 (30 g/fish) 175-C*.sup.10 99 Eel 500 70 D 1 93.6 (30 g/fish) 175-D 92.5 7.5 Eel 500 70 D 1 94.2 (30 g/fish) 175-E*.sup.11 92.5 Eel 500 70 D 1 94.0 (30 g/fish) Comparative Example 70 10 90 Eel 500 70 D 1 88.6 (30 g/fish) 71 50 50 Eel 500 70 D 1 88.0 (30 g/fish) 72 99 1 Eel 500 70 D 1 87.0 (30 g/fish) 73 92.5 7.5 Eel 500 70 D 1 88.4 (30 g/fish) __________________________________________________________________________ *Kind of Base Feed Stuff *.sup.10 In addition to 99 wt. % of glucose, 1 wt. % of powdery tomato is mixed. *.sup.11 In addition to 92.5 wt. % of glucose, 7.5 wt. % of powdery tomat is mixed.

TABLE 4-(iii) __________________________________________________________________________ Mix proportions of each component in pharma- ceutical composition for animals (wt. %) Kind Number Feed Feed per Fumaric Powdery of of periods day Glucose acid Vinegar tomato fish fishes (days) K.B.F.S.* (times) P.G.A. __________________________________________________________________________ (%) Example 176 10 50 30 10 Eel 500 70 D 1 94.4 (30 g/fish) 177 10 50 15 25 Eel 500 70 D 1 93.8 (30 g/fish) 178 88 1 1 10 Eel 500 70 D 1 94.2 (30 g/fish) 179 98.9 1 0.1 Eel 500 70 D 1 94.0 (30 g/fish) 180 88.75 7.5 2.5 1.25 Eel 500 70 D 1 96.8 (30 g/fish) Comparative Example 74 10 90 Eel 500 70 D 1 87.4 (30 g/fish) 75 50 50 Eel 500 70 D 1 88.8 (30 g/fish) 76 99 1 Eel 500 70 D 1 86.2 (30 g/fish) __________________________________________________________________________ *Kind of Base Feed Stuff

TABLE 4-(iv) __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Num- Feed Cit- Powdery flesh Lactobiacillus Kind ber Feed per ric Vine- of Japanese bifidus of of periods day Sugar acid gar apricot (cells/100 g) fish fishes (days) K.B.F.S.* (times) P.G.A. __________________________________________________________________________ Example 181 90 7.5 2.5 Horse mackerel 200 140 E 4 97.0 (40 g/fish) 182 91.25 7.5 1.25 Horse mackerel 200 140 E 4 97.0 (40 g/fish) 183 88.75 7.5 2.5 1.25 Horse mackerel 200 140 E 4 98.5 (40 g/fish) 184-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Horse mackerel 200 140 E 4 97.0 (40 g/fish) 184-B 92.5 7.5 2.5 .times. 10.sup.8 /100 g Horse mackerel 200 140 E 4 96.5 (40 g/fish) 184-C 92.5 7.5 Horse mackerel 200 140 E 4 96.0 (40 g/fish) Comparative Example 77 92.5 7.5 Horse mackerel 200 140 E 4 78.5 (40 g/fish) Example 185 96.9 3 0.1 Horse mackerel 200 140 E 4 93.5 (40 g/fish) 186 96.9 3 0.1 Horse mackerel 200 140 E 4 93.5 (40 g/fish) 187 96.8 3 0.1 0.1 Horse mackerel 200 140 E 4 94.0 (40 g/fish) 188-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Horse mackerel 200 140 E 4 94.0 (40 g/fish) 188-B 97 3 Horse mackerel 200 140 E 4 93.0 (40 g/fish) 188-C 97 3 2.5 .times. 10.sup.8 /100 g Horse mackerel 200 140 E 4 92.5 (40 g/fish) Comparative Example 78 97 3 Horse mackerel 200 140 E 4 81.5 (40 g/fish) Example 189 40 25 15 20 Horse mackerel 200 140 E 4 96.5 (40 g/fish) 190-A 60 25 15 Horse mackerel 200 140 E 4 96.0 (40 g/fish) 190-B 75 25 2.5 .times. 10.sup.10 /100 g Horse mackerel 200 140 E 4 95.5 (40 g/fish) 190-C 75 5 20 Horse mackerel 200 140 E 4 95.0 (40 g/fish) Comparative Example 79 75 25 Horse mackerel 200 140 E 4 80.0 (40 g/fish) Example 191 90 7.5 2.5 Yellowtail 200 350 F 4 97.5 (20 g/fish) 192 91.25 7.5 1.25 Yellowtail 200 350 F 4 97.0 (20 g/fish) 193 88.75 7.5 2.5 1.25 Yellowtail 200 350 F 4 97.5 (20 g/fish) 194-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g Yellowtail 200 350 F 4 97.0 (20 g/fish) 194-B 92.5 7.5 Yellowtail 200 350 F 4 96.0 (20 g/fish) 194-C 92.5 7.5 2.5 .times. 10.sup.8 /100 g Yellowtail 200 350 F 4 96.5 (20 g/fish) Comparative Example 80 92.5 7.5 Yellowtail 200 350 F 4 85.0 (20 g/fish) Example 195 96.9 3 0.1 Yellowtail 200 350 F 4 95.0 (20 g/fish) 196 96.9 3 0.1 Yellowtail 200 350 F 4 94.5 (20 g/fish) 197 96.8 3 0.1 0.1 Yellowtail 200 350 F 4 95.0 (20 g/fish) 198-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g Yellowtail 200 350 F 4 95.0 (20 g/fish) 198-B 97 3 2.5 .times. 10.sup.8 /100 g Yellowtail 200 350 F 4 93.5 (20 g/fish) 198-C 97 3 Yellowtail 200 350 F 4 94.0 (20 g/fish) Comparative Example 81 97 3 Yellowtail 200 350 F 4 87.0 (20 g/fish) Example 199 40 25 15 20 Yellowtail 200 350 F 4 97.0 (20 g/fish) 200-A 60 25 15 2.5 .times. 10.sup.10 /100 g Yellowtail 200 350 F 4 96.0 (20 g/fish) 200-B 75 25 Yellowtail 200 350 F 4 96.0 (20 g/fish) 200-C 75 5 20 2.5 .times. 10.sup.10 /100 g Yellowtail 200 350 F 4 95.5 (20 g/fish) Comparative Example 82 75 25 Yellowtail 200 350 F 4 86.5 (20 g/fish) __________________________________________________________________________ *Kind of Base Feed Stuff

EXAMPLES 201-210 AND COMPARATIVE EXAMPLES 83-85

Components shown in Table 5 below were mixed in accordance with weight proportions shown in Table 5 to prepare pharmaceutical compositions for animals of the present Examples 201-210 and Comparative Examples 83-85. 0.5 g of each of the pharmaceutical compositions were added to 100 g of Base Feed Stuff G shown below to prepare test feed stuffs. Each of the test feed stuffs was fed freely to milk cows of one month old for 60 days. The amount of milk obtained was determined every day and the mean milk fat was determined every ten days. The results are shown in Table 5 below.

______________________________________ Composition of Base Feed Stuff G ______________________________________ Corn 40.0 weight parts Milo 13.5 weight parts Wheat flour 18.0 weight parts Barley 6.5 weight parts Wheat bran 9.0 weight parts Soybean oil meal 7.0 weight parts Molasses 2.7 weight parts Calcium carbonate 1.5 weight parts Calcium phosphate 1.1 weight parts Salt 0.5 weight parts Vitamins 0.1 weight parts Minerals 0.1 weight parts ______________________________________

TABLE 5 __________________________________________________________________________ Mix proportions of each component in pharmaceutical composition for animals (wt. %) Powdery flesh Lactobacillus Citric of Japanese bifidus Amount of milk/ Sugar acid Vinegar apricot (cells/100 g) day. cow (kg) M.M.F. (%) __________________________________________________________________________ Example 201 90 7.5 2.5 23.3 3.8 202 91.25 7.5 1.25 23.6 3.8 203 88.75 7.5 2.5 1.25 24.6 3.8 204-A 90 7.5 2.5 2.5 .times. 10.sup.8 /100 g 24.0 3.8 204-B 92.5 7.5 23.0 3.8 204-C 92.5 7.5 22.9 3.8 Comparative Example 83 92.5 7.5 17.9 3.4 Example 205 96.9 3 0.1 22.0 3.7 206 96.9 3 0.1 22.4 3.8 207 96.8 3 0.1 0.1 23.3 3.7 208-A 96.9 3 0.1 2.5 .times. 10.sup.8 /100 g 23.0 3.7 208-B 97 3 21.8 3.7 208-C 97 3 21.5 3.7 Comparative Example 84 97 3 18.2 3.4 Example 209 40 25 15 20 21.8 3.7 210-A 60 25 15 2.5 .times. 10.sup.10 /100 g 22.3 3.7 210-B 75 25 21.0 3.7 210-C 75 5 20 22.0 3.7 Comparative Example 85 75 25 18.4 3.4 __________________________________________________________________________

EXAMPLE 211

88.75 weight parts of sugar, 7.5 weight parts of citric acid, 2.5 weight parts of vinegar and 1.25 weight parts of powdery flesh of Japanese apricot were mixed to prepare a pharmaceutical composition for broilers. 0.7 g of the pharmaceutical composition was added to 100 g of Base Feed Stuff H shown below, and the mixture was then fed freely to 2500 heads of broilers from 15 to 8 days before their forwarding.

Then, 1.28 g of said pharmaceutical composition was added to 100 g of said Base Feed Stuff H, and fed freely to said 2500 heads of broilers during 7 days before their forwarding.

______________________________________ Composition of Base Feed Stuff H ______________________________________ Corn 450 weight parts Milo 260 weight parts Soybean meal 190 weight parts Fish powder 50 weight parts Yellow grease 30 weight parts Calcium carbonate 7 weight parts Calcium triphosphate 6 weight parts Salt Premix vitamins & minerals 5 weight parts ______________________________________

Said broilers were butchered and the quality of their bodies and meats was observed, and the yield of meats was determined. On the other hand, said Base Feed Stuff H not containing the pharmaceutical composition of the present invention was feed to other 2500 broilers in the same manner as above. These broilers were butchered, and the quality of their bodies and meats was also observed and the yield was determined.

The results are as follows:

Bodies of butchered broilers bred with the pharmaceutical composition of the present invention (called hereinafter "present broilers") had better skin color and had tighter bodies than those of the comparative broilers.

The yield of butchered broilers' bodies of the present broilers was 1.5% larger than that of the comparative broilers.

The thigh and breast of the present broilers had a beautiful pinkish color and were less fatty and were more resilient than those of the comparative broilers. The stomachs of the present broilers were less fatty.

The total weight of the fatty livers of the present 1200 broilers was 1.6 kg, and this was one third of that of 1200 comparative broilers. The yield of dissectioned bodies, which were dissectioned according to IV type-dissection of Japanese Agriculture Standard, was 40.0% in case of the present broilers. On the other hand, in case of the comparative broilers, that was 37.6%.

Breasts of the broilers were frozen in the temperature range of -3.degree. C. to -8.degree. C. for 81 hours, and then were thawed. Breasts of the present broilers were not hard. On the other hand, those of the comparative broilers were hard. Then, these thawed breasts were maintained at 16.degree. C. for 20 minutes. The breasts of the present broilers had less drips than those of the comparative broilers.

EXAMPLE 212

In this example, the pharmaceutical composition for broilers prepared in the above Example 211 was used for beef cattles.

The pharmaceutical composition was fed to beef cattles of 490 days old 36 g per beef cattle per day together with the Base Feed Stuff I shown below.

______________________________________ Base Feed Stuff I ______________________________________ Corn 40.0 weight parts Milo 13.5 weight parts Wheat flour 18.0 weight parts Barley 6.5 weight parts Wheat bran 9.0 weight parts Soybean oil meal 7.0 weight parts Molasses 2.7 weight parts Calcium carbonate 1.5 weight parts Calcium phosphate 1.1 weight parts Salt 0.5 weight parts Vitamins 0.1 weight parts Minerals 0.1 weight parts ______________________________________

The beef cattles were butchered and dissectioned, and then the quality of the beef was observed. The beef of the present beef cattles had clearer red color than the comparative beef cattles. In the beef of the present beef cattles, fats were contained in dense and marble-like states.