Biological Activities of Triterpenoids and Phenolic Compounds from Myrica cerifera Bark.

Seven triterpenoids, 1 - 7, two diarylheptanoids, 8 and 9, four phenolic compounds, 10 - 13, and three other compounds, 14 - 16, were isolated from the hexane and MeOH extracts of the bark of Myrica cerifera L. (Myricaceae). Among these compounds, betulin (1), ursolic acid (3), and myricanol (8) exhibited cytotoxic activities against HL60 (leukemia), A549 (lung), and SK-BR-3 (breast) human cancer cell lines (IC50 3.1 - 24.2 μm). Compound 8 induced apoptotic cell death in HL60 cells (IC50 5.3 μm) upon evaluation of the apoptosis-inducing activity by flow cytometric analysis and by Hoechst 33342 staining method. Western blot analysis on HL60 cells revealed that 8 activated caspases-3, -8, and -9 suggesting that 8 induced apoptosis via both mitochondrial and death receptor pathways in HL60. Upon evaluation of the melanogenesis-inhibitory activity in B16 melanoma cells induced with α-melanocyte-stimulating hormone (α-MSH), erythrodiol (7), 4-hydroxy-2-methoxyphenyl β-d-glucopyranoside (13), and butyl quinate (15) exhibited inhibitory effects (65.4 - 86.0% melanin content) with no, or almost no, toxicity to the cells (85.9 - 107.4% cell viability) at 100 μm concentration. In addition, 8, myricanone (9), myricitrin (10), protocatechuic acid (11), and gallic acid (12) revealed potent DPPH radical-scavenging activities (IC50 6.9 - 20.5 μm).