[Isolation-preparation and determination of chromones from Saposhnikovia divaricata].
Kata kunci
Abstrak
OBJECTIVE
To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.
METHODS
The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).
RESULTS
All the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.
CONCLUSIONS
The method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.
