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Proline biosynthesis: multiple defects in Chinese hamster ovary cells.
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Thrombin receptor polymorphism in Chinese hamster.
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We report the existence of a new Chinese hamster thrombin receptor allele, characterized by an in-frame insertion of three nucleotides at position 250 of the published sequence. As a consequence, an additional proline is inserted into a proline-rich region of the extracellular amino-terminal domain
Salivary proline-rich protein polymorphisms in Chinese, Malays and Indians in Singapore.
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Salivary proline-rich protein (PRP) polymorphism, PRH1, PRH2, Ps, Pm (PmF), PmS and Gl, were investigated in three ethnic groups in Singapore: Chinese, Malays and Indians. The phenotype and gene frequencies were presented and comparison with other ethnic groups was made. The As protein, which was
Allelic variants of acidic proline-rich proteins observed in Japanese, Chinese, and Malays.
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Three new variants of acidic proline-rich proteins (At, Au, Aw) were found in human parotid saliva by isoelectric focusing and basic gel electrophoresis. Electrophoretic comparison of the purified proteins and their tryptic peptides suggested minor charge and size differences from other acidic PRPs.
[Mutational analysis of three Chinese pedigrees with adrenoleukodystrophy].
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OBJECTIVE
To identify the mutational genotype of three Chinese families with X-linked adrenoleukodystrophy (X-ALD: MIM#300100).
METHODS
Total RNA was extracted from the peripheral blood leukocytes of patients 1, 2 and the mother of patient 3, using RNA blood Mini kit (QIAGEN). After reverse
[Genetic polymorphism of the PRODH and 5-HT(2A) receptor gene in Korean-Chinese and Han nationality in Chinese Yanbian area].
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OBJECTIVE
To investigate the polymorphisms distribution of genes related with mental diseases, such as proline dehydrogenase gene (PRODH) and serotonin 2A(5-HT(2A)) receptor gene, among Korean-Chinese and Han nationality in Chinese Yanbian area.
METHODS
By utilizing techniques of polymerase chain
Variant Chinese hamster cells resistant to the proline analog L-azetidine 2-carboxylic acid.
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Variants resistant to the toxic effects of the proline analog L-azetidine 2-carboxylic acid (AZCA) have been isolated from the Chinese hamster tissue culture line G3 by a three-step selection procedure using increasing concentrations of AZCA. Cells surviving each of the three selective steps have
Novel RUNX2 mutations in Chinese individuals with cleidocranial dysplasia.
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Cleidocranial dysplasia (CCD) is an inherited autosomal-dominant skeletal disease caused by heterozygous mutations in the osteoblast-specific transcription factor, RUNX2. We performed mutation analysis of RUNX2 on four unrelated Chinese individuals with CCD. Three novel distinct mutations were
Evidence for allelic exclusion in Chinese hamster ovary cells.
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Earlier results suggested that the functional hemizygosity of genes in pseudodiploid Chinese hamster ovary (CHO) cells is due to the silencing of one allele by DNA methylation. From this one could make a strong prediction that we have now been able to confirm by genetic experiments, using thymidine
Proline incorporation into protein by Rickettsia prowazekii during growth in Chinese hamster ovary (CHO-K1) cells.
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Both the requirement of Rickettsia prowazekii for the amino acid proline for growth and rickettsial proline incorporation were determined in Chinese hamster ovary (CHO-K1) cells auxotrophic for proline. Incubation of cells in Dulbecco modified Eagle medium supplemented with various concentrations of
Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells
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Chinese hamster ovary (CHO) cells are the superior host cell culture models used for the bioproduction of therapeutic proteins. One of the prerequisites for bioproduction using CHO cell lines is the need to generate stable CHO cell lines with optimal expression output. Antibiotic selection is
Biochemical characterization of azetidine carboxylic acid-resistant Chinese hamster cells.
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We have isolated variants of Chinese hamster lung (CHL) fibroblasts with decreased sensitivity to the toxic proline analog, L-azetidine-2-carboxylic acid (AZCA). Resistance to AZCA is a stable characteristic of these cell lines since it is maintained after 40 generations in nonselective medium. Two
Precore sequence variation in Chinese isolates of hepatitis B virus.
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Direct sequencing of polymerase chain reaction-amplified serum hepatitis B virus (HBV) DNA was used to characterize the precore region of HBV from Chinese patients with chronic hepatitis. Two types of mutually exclusive variants were found in hepatitis B e antigen (HBeAg)-negative patients. The
Characterization of a mutator gene in Chinese hamster ovary cells.
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We have recently reported the isolation of a class of mutants (called thy-) that is both resistant to arabinosyl cytosine and auxotrophic for thymidine. thy- mutants have a 5- to 10-fold elevated pool of dCTP and are deficient in the synthesis of dTTP as an apparent consequence of a single mutation
A search for allelic recombination in Chinese hamster cell hybrids.
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Mutants resistant to 6-thioguanine were selected from CHO cells which were either temperature sensitive or proline requiring. These mutants were stable and had low levels of hypoxanthine guanine phosphoribosyl transferase (HGPRT). Hybrids were selected which were heteroallelic at the hgprt locus and
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