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cystathionine/brasika
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Overexpression of cystathionine-gamma-synthase enhances selenium volatilization in Brassica juncea.
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Selenium (Se) can be assimilated and volatilized via the sulfate assimilation pathway. Cystathionine-gamma-synthase (CGS) is thought to catalyze the synthesis of Se-cystathionine from Se-cysteine, the first step in the conversion of Se-cysteine to volatile dimethylselenide. Here the hypothesis was
Biochemical characterization of cystine lyase from broccoli (Brassica oleracea var. italica).
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Cystine lyase is the enzyme responsible for off-aroma deterioration in fresh unblanched broccoli. In this research, cystine lyase purification from broccoli has been optimized. Only one protein peak with cystine lyase activity was found during purification. Broccoli cystine lyase was purified
Purification and Characterization of Cystine Lyase a from Broccoli Inflorescence.
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One of the three isoforms of an enzyme degrading L-cystine was purified to homogeneity from broccoli (Brassica oleracea var. italica) inflorescences, with use of a sensitive assay based on derivatization of a reaction product with monobromobimane. The reaction product with a thiol group was found to
The formation of sulphur-containing amino acids in germinating seeds of rape (Brassica napus L.).
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Sodium [(35)S]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label
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