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griffonia simplicifolia/phosphatase
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Enzyme-linked lectin assay (ELLA): use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of alpha-D-galactopyranosyl end groups.
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Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound natural and synthetic glycoproteins bearing alpha-D-galactopyranosyl end
Expression of muscle capillary alkaline phosphatase is affected by hypoxia.
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Hypoxia stimulates angiogenesis in some microvascular beds, but no clear angiogenic effect of hypoxia has yet been demonstrated in adult skeletal muscle. In this study the distribution of alkaline phosphatase (APase) was compared with a novel microvascular marker, Griffonia simplicifolia I (GSI), to
Cytochemistry for lectins, actin, nucleotide tetrazolium reductases and several phosphatases in the porcine semitendinosus muscle: vascular development in young pigs.
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An ontogeny study of the porcine semitendinosus muscle was conducted to meet two objectives: 1) to evaluate enzyme histochemistry, lectin cytochemistry and actin staining for usefulness as quantitative markers of muscle capillaries and 2) to describe the ontogeny of capillary density changes in
Lectin binding in skeletal muscle. Evaluation of alkaline phosphatase conjugated avidin staining procedures.
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Cryostat sections from rat gracilis muscles were incubated with different biotinylated lectins: Con A (Concanavilin A), WGA (Wheat germ agglutinin), SBA (soybean agglutinin), GS I and GS II (Griffonia simplicifolia agglutinin), LCA (Lens culinaris agglutinin), PNA (peanut agglutinin) and PSA (Pisum
Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells.
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Rat brain microvessel endothelial cells were immortalized by transfection with a plasmid containing the E1A adenovirus gene. One clone, called RBE4, was further characterized. These cells display a nontransformed phenotype and express typical endothelial markers, Factor VIII-related antigen and
Griffonia simplicifolia I: fluorescent tracer for microcirculatory vessels in nonperfused thin muscles and sectioned muscle.
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Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly
A review of studies in our laboratory regarding ELLA methodology for the study of cell surface carbohydrates from tumors of varying metastatic potential.
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An enzyme-linked lectin assay, termed ELLA, has been developed to investigate differences in cell surface carbohydrate groups on the surface of murine fibrosarcoma cells of varying metastatic potential. We have conjugated alkaline phosphatase to Griffonia simplicifolia I-B4 and have obtained an
Combined retrograde tracing and enzyme/immunohistochemistry of trigeminal ganglion cell bodies innervating tooth pulps in the rat.
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Rat trigeminal neurons innervating tooth pulps were retrogradely labelled with fluorogold and analysed enzyme- and immunohistochemically for their content of substance P, calcitonin gene-related peptide, fluoride-resistant acid phosphatase, GM 1 ganglioside, carbonic anhydrase and neurofilament
Induction of angiogenesis by lidocaine and basic fibroblast growth factor: a model for in vivo retroviral-mediated gene therapy.
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A strategy of direct, in vivo retroviral-mediated gene therapy targeting capillary endothelial cells must provide an environment of active angiogenesis. Both lidocaine and basic fibroblast growth factor (bFGF) promote angiogenesis, but the angiogenic response invoked by these substances in normal
Embryonal carcinoma in the abdominal cavity of a male calf.
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An embryonal carcinoma was diagnosed in the abdominal cavity of a 55-day-old male calf. Macroscopically, a large volume of ascitic fluid was observed along with white to yellowish-white masses of various sizes densely located on the abdominal wall and the surface of abdominal organs. There was an
Lectin and neuropeptide labeling of separate populations of dorsal root ganglion neurons and associated "nociceptor" thin axons in rat testis and cornea whole-mount preparations.
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As part of a program to explore patterns of innervation by nociceptor-related thin sensory axons in a variety of peripheral regions, we have labeled calcitonin gene-related peptide immunoreactive (CGRP-IR) nerve fibers in whole mounts of rat testicular tunica vasculosa and cornea. Efforts were
Identification of capillaries in sections from skeletal muscle by use of lectins and monoclonal antibodies reacting with histo-blood group ABH antigens.
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This study was performed to evaluate the application of different lectins and monoclonal antibodies against ABH antigens to detect and characterize carbohydrate structures in capillaries of skeletal muscle from humans and laboratory animals. Blood group specific lectins (Griffonia simplicifolia,
Effects of hypoxia at different life stages on locomotory muscle phenotype in deer mice native to high altitudes.
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Animals native to high altitude must overcome the constraining effects of hypoxia on tissue O2 supply to support routine metabolism, thermoregulation in the cold, and exercise. Deer mice (Peromyscus maniculatus) native to high altitude have evolved an enhanced aerobic capacity in hypoxia, along with
Sub-populations of smaller diameter trigeminal primary afferent neurons defined by expression of calcitonin gene-related peptide and the cell surface oligosaccharide recognized by monoclonal antibody LA4.
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Immunocytochemistry has been used to examine the trigeminal ganglion cell populations in the rat which expresses calcitonin gene-related peptide (CGRP) and the oligosaccharide antigen recognized by the monoclonal antibody LA4. Calcitonin gene-related peptide and LA4 identify two large but mainly
Transganglionic transport and binding of the isolectin B4 from Griffonia simplicifolia I in rat primary sensory neurons.
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The isolectin B4 from Griffonia simplicifolia I binds to a subpopulation of rat small-diameter dorsal root ganglion neurons, and to fibres and presumed terminals in laminae I-II of the spinal cord dorsal horn. In the present study we investigated B4 and B4 conjugated to horseradish peroxidase as
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