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13 hasil
Ulex europaeus 1 lectin targets microspheres to mouse Peyer's patch M-cells in vivo.
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The interaction of latex microspheres with mouse Peyer's patch membranous M-cells was studied in a mouse gut loop model after the microspheres were coated with a variety of agents. Carboxylated microspheres (diameter 0.5 micron) were covalently coated with lectins Ulex europaeus 1, Concanavalin A,
A novel quantification of blood-brain barrier damage and histochemical typing after embolic stroke in rats.
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Treatment strategies in acute ischemic stroke are still limited. Considering numerous translation failures, research is tending to a preferred use of human-like animal models, and a more-complex perspective of tissue salvaging involving endothelial, glial and neuronal components according to the
Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.
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A fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to
Retinal pigment epithelial damage, breakdown of the blood-retinal barrier, and retinal inflammation in dogs with primary glaucoma.
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OBJECTIVE
This paper aims to determine if abnormalities of the retinal pigment epithelium (RPE) and retinal inflammation occur in primary glaucoma.
METHODS
Twenty-three canine globes with primary glaucoma, goniodysgenesis, and elevated intraocular pressure were evaluated. Sections from 6 control and
Microvessel loss, vascular damage and glutamate redistribution in the retinas of dogs with primary glaucoma.
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OBJECTIVE
Vascular damage and ischemia-like changes in glutamate distribution occur in primary glaucoma (PG) in dogs. We measured the microvessel density in PG retinas to determine whether microvessel loss may induce ischemia and glutamate redistribution.
METHODS
Sections from 12 control and 33
Histochemical characteristic of perivascular space in the brain with an advanced edema.
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Amorphorous and colorless spaces, Virchow-Robin spaces (VRS), were often found by HE stain around blood vessels in the edematous brain. Histochemical characteristic of the enlarged VRS caused by an advanced edema and detected by lectin stain using Griffonia simplicifolia I agglutinin in the brain
Binding of Griffonia simplicifolia 1 isolectin B4 (GS1 B4) to alpha-galactose antigens.
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Glycoconjugates with terminal Galalpha1-3Galbeta1-4GlcNAc sequences (alpha-galactosyl epitopes, natural xenoreactive antigens) are present on various tissues in pigs and are recognized by human anti-alphagalactosyl (alphaGal) antibodies1. Hence xenotransplantation (pig-to-human) would trigger immune
Comparative study of blood group-recognizing lectins toward ABO blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides.
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Binding specificities of ABO blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. Human serum
Cellular mechanisms of blood-retinal barrier dysfunction in macular edema.
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OBJECTIVE
To determine the mechanism of blood-retinal barrier (BRB) dysfunction in human and experimental specimens using immunocytochemistry.
METHODS
Extravascular albumin was localized in clinical specimens and retinas from transgenic mice that overexpress vascular endothelial growth factor (VEGF)
Defect of sinusoidal Fc receptors and immune complex uptake in CCl4-induced liver cirrhosis in rats.
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The purpose of this paper is to provide a histopathologic basis for abnormalities in immune-complex clearance in liver disease. Fc receptors in CCl4-induced liver cirrhosis in rats were studied by applying peroxidase-antiperoxidase immunoglobulin G complex as a ligand to the frozen sections.
Purification and characterization of mouse interferon with novel affinity sorbents.
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Several novel selective sorbents for mouse interferon are described that exploit the hydrophobic property and glycoprotein nature of this molecule. Low-molecular-weight ligands (hydrocarbons) and high-molecular-weight ligands (bovine serum albumin) immobilized on agarose bind selectively mouse
Segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium.
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The barrier function, surface biochemistry, and morphology of confluent monolayers of endothelial cells isolated from different segments of the bovine lung vasculature [microvessels (BLMVEC), vein (BPVEC) and artery (BPAEC)] were grown in culture and compared. A number of common cell surface
Impairment of myocardial angiogenic response in the absence of osteopontin.
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OBJECTIVE
Osteopontin (OPN), increased in the heart following myocardial infarction (MI), plays an important role in post-MI remodeling. Angiogenesis, an important feature of tissue repair, begins in the infarcted myocardium within 3 days post-MI. Here, the authors studied the role of OPN in
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