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isopentenyl adenosine/jagung
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High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica.
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Cytokinin oxidase/dehydrogenase (CKO/CKX) is a flavoenzyme, which irreversibly inactivates cytokinins by severing the isoprenoid side chain from the adenine/adenosine moiety. There are several genes coding for the enzyme in maize (Zea mays). A Z. mays CKO1 cDNA was cloned in the yeast Yarrowia
trans-Ribosylzeatin: Its Biosynthesis in Zea mays Endosperm and the Mycorrhizal Fungus, Rhizopogon roseolus.
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When [8-(14)C]-N(6)-(Delta(2)-isopentenyl) adenosine is incubated with the endosperm of corn (2 weeks after pollination), it is converted to [(14)C]-N(6)-(4-hydroxy-3-methylbut-2-trans-enyl) adenosine, trans-ribosylzeatin. This biosynthetic step, N(6)-(Delta(2)-isopentenyl) adenosine to
A highly selective biosensor with nanomolar sensitivity based on cytokinin dehydrogenase.
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We have developed a N6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins. Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface
Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.
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Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an
Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro.
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Endogenous hormone levels (indole-3-acetic acid [IAA], abscisic acid [ABA], gibberellins (1, 3) and (20) [GAs], zeatin/zeatin riboside [Z/ZR] and N(6)[Delta(2)-isopentenyl] adenine/N(6)[Delta(2)-isopentenyl] adenosine; [iP/iPA]) were analysed in immature maize zygotic embryos of two maize (Zea mays
A response-regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize.
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A cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated utilizing the differential display technique, and studied using the expression of ZmCip1 in nitrogen-starved maize plants. The cloned cDNA contained an open reading frame consisting of 157 amino acids with
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