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Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants.
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In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated
An anti-B lectin from Zea mays everta.
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The results of serological studies of Zea mays everta seed extracts with anti-B specificity are presented. Lectin will agglutinates A1B erythrocytes significantly more weakly than erythrocytes of B and A2B blood groups.
Purification and characterization of a galactose-specific lectin from corn (Zea mays) coleoptile.
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We purified and characterized a lectin from the corn coleoptyle (Zea mays). The lectin (CCL) was purified by affinity chromatography on a Lactosyl-Sepharose 4B column. It is a glycoprotein of 88.7 kDa, composed mainly by glutamic, aspartic, glycine, and Ser residues; in a minor proportion, it
Differences in the mannose oligomer specificities of the closely related lectins from Galanthus nivalis and Zea mays strongly determine their eventual anti-HIV activity.
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BACKGROUND
In a recent report, the carbohydrate-binding specificities of the plant lectins Galanthus nivalis (GNA) and the closely related lectin from Zea mays (GNAmaize) were determined by glycan array analysis and indicated that GNAmaize recognizes complex-type N-glycans whereas GNA has
Analysis of the lectins from teosinte (Zea diploperennis) and maize (Zea mays) coleoptiles.
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To identify molecular evidence of the common origin of maize and teosinte, a lectin from teosinte coleoptile (TCL) was purified, through affinity chromatography on a lactosyl-Sepharose column, and some of the physicochemical parameters were compared with those from the maize coleoptile lectin (CCL).
Cytokeratin, lectin, and acidic mucin modulation in differentiating colonic epithelial cells of mice after feeding Western-style diets.
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Several studies have recently reported the development of colonic epithelial cell hyperproliferation in rodents following the ingestion of Western-style diets. In this study, additional measurements related to differentiation and maturation of the colonic epithelial cells were made after feeding
Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs.
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Gamete transport, fertilization, and early embryonic development take place in different regions of the oviduct and under different hormonal conditions. The objective of this study was to use lectins to detect variation in the distribution of glycosylated molecules on the surface of the epithelia
Specific interactions between lectins and red blood cells of Chornobyl cleanup workers as indicator of some late radiation effects.
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OBJECTIVE
Growing interest in lectins is based on their diagnostic and pharmacological potential, especially the ability to inhibit proliferation and initiate apoptosis of cancer cells. In our research microplate lectinoassay able to detect carbohydrate containing structures (receptors) on
Applicability of lectin histochemistry in a test system with in ovo treatment for detecting androgenic and antiandrogenic effects of chemicals in Japanese quail (Coturnix japonica).
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The aim of the current study was to examine whether analysis of the appearance of specific lectin-positive substances in the quail embryonic cloacal gland would be useful for evaluating the androgenic and antiandrogenic effects of chemicals. Fertilized Japanese quail eggs were injected with 0 to 75
Effect of plant lectins on Ustilago maydis in vitro.
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Ustilago maydis is an edible parasitic basidiomycete, which specifically infects corn (Zea mays) and teocintle (Z. diploperennis). To characterise the interaction between the basidiomycete and its host organism, we tested the effect of plant lectins with well-known sugar specificity on the growth
Ultrastructural detection of fucosyl residues at the surface of axenically grown maize roots/sequential use of UeA lectin and fucosyl ferritin as the specific glycosylated marker.
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This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the
Salt-soluble lectins of corn grain.
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Lectins extracted from corn (Zea mays L.) kernel with Tris-HCl buffer pH 7.5 were isolated from the crude extract by affinity chromatography on Sepharose 6B-N-acetyl-d-galactosamine and Sepharose 6B-methylalpha-d-mannoside, and also by lectin affinity chromatography using concanavalin A and Lens
Characterization of the lectin from the bulbs of Eranthis hyemalis (winter aconite) as an inhibitor of protein synthesis.
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The lectin from Eranthis hyemalis has been previously characterized as consisting of two polypeptide chains covalently linked by disulfide bond(s) (Cammue, B. P., Peeters, B., and Peumans, W. J. (1985) Biochem. J. 227, 949-955). We have further characterized the biochemical properties of the lectin
Evolution and structural diversification of Nictaba-like lectin genes in food crops with a focus on soybean (Glycine max).
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The Nictaba family groups all proteins that show homology to Nictaba, the tobacco lectin. So far, Nictaba and an Arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. The availability of more than 50 sequenced plant genomes provided the opportunity for a
Induction of cytoplasmic mannose-binding jacalin-related lectins is a common phenomenon in cereals treated with jasmonate methyl ester.
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Treatment of whole plants with jasmonic acid methyl ester induces lectin activity in leaves of Oryza sativa, Hordeum vulgare, Triticum vulgare, Secale cereale and Zea mays. Purification and characterization of the lectins revealed that they all have a very similar molecular structure and
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