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phytohaemagglutinin/sarkoma
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Identification of an anti-human osteogenic sarcoma monoclonal-antibody-defined antigen on mitogen-stimulated peripheral blood mononuclear cells.
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An anti-human osteogenic sarcoma monoclonal antibody (mouse IgG2b) termed 791T/36 was found to exert complement-dependent cytotoxicity against phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear (PBMN) cells. This reaction was examined by flow cytofluorimetry using indirect membrane
Growth stage dependent expression of MHC antigens on the canine transmissible venereal sarcoma.
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Canine transmissible venereal sarcoma (CTVS) is a naturally occurring contagious neoplasm which can be transplanted with intact viable cells across major histocompatibility (MHC) barriers within the species and even to other members of the canine family, such as foxes, coyotes, and wolves. After 2
Cell-mediated immunity to moloney sarcoma virus in mice. I. Analysis of antigens responsible for lymphocyte stimulation in regressor mice.
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Purified viruses, viral antigens, and cell extracts have been tested for their ability to stimulate protein synthesis by MSV-M-1 regressor spleen cells from BALB/c mice. Immune, but not normal cells, responded to MSV-M, but not to MSV-G virus-1, and to the type-specific viral envelope glycoprotein
Infectious human herpesvirus 8 in a healthy North American blood donor.
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BACKGROUND
Molecular studies have provided strong evidence for the association of human herpesvirus 8 (HHV-8) with Kaposi's sarcoma. These data have been supported by serological studies, which have also suggested that HHV-8 can be found in the healthy population. We report the presence of
Quantification of non-specific immunosuppressive factors.
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A simple, reproducible and quantitative method for evaluating certain non-specific immunological inhibitors in a variety of biological fluids is described. Human lymphocytes were stimulated with PHA in the presence of colchicine. Phytohaemagglutinin stimulated a large percentage of cells and
IgD production and other lymphocyte functions in HIV infection: immaturity and activation of B cells at different clinical stages.
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T and B cell function, in particular IgD production in vitro, were studied across the spectrum of HIV infection in homosexual men and compared with seronegative homosexual and heterosexual male controls. Proliferation to phytohaemagglutinin (PHA) was reduced most strikingly in symptomatic HIV
Quantification of soluble E-receptor in the serum of patients with various diseases and its accompanying in vitro immunosuppression in neoplasia.
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Evidence in the literature indicates that soluble E-receptor in the serum may modulate T-dependent immune response. We have developed a solid-phase radioimmunoassay to measure the soluble form of E-receptor from various sources. The assay detects solubilized antigens derived from E-receptor-bearing
Immunity in tumours of the skin. Studies on cell-mediated immune reactions.
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Cell-mediated immunity (CMI) was investigated in a series of patients with epithelial tumours of the skin and with Kaposi's sarcoma. In all 53 patients the peripheral blood lymphocyte count showed no substantial difference from the control values. The blasts count after phytohaemagglutinin
Purine metabolism and control of cell proliferation.
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Exposure of normal lymphocytes to phytohaemagglutinin or other lectin mitogens results in increased concentrations of 5-phosphoribosyl-1-pyrophosphate (PP-ribose-P) within minutes. Subsequently, synthesis of purine nucleotides by both the de novo and the salvage pathways is facilitated. This change
Effect of phorbol myristate acetate on T cell colony formation, interleukin-2 (IL-2) receptor expression and IL-2 production by cells from patients at all stages of HIV infection.
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We and others have shown that several T cell responses induced by the mitogen phytohaemagglutinin (PHA), including T cell colony formation, IL-2 receptor (IL-2R) expression, and IL-2 production are impaired in patients with AIDS and lymphadenopathy syndrome (LAS). We investigated whether phorbol
Inhibition by human T-lymphotropic virus (HTLV-I) of T-lymphocyte mitogenesis: failure of exogenous T-cell growth factor to restore responsiveness to lectin.
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Various retroviruses, including human T-cell lymphotropic virus (HTLV-I) and avian sarcoma virus (ASV), can prevent coincubated human peripheral lymphocytes from responding efficiently to phytohaemagglutinin. Addition of high concentrations of T-cell growth factor (TCGF) activity usually overcomes
Expression of deoxycytidine kinase and phosphorylation of 2-chlorodeoxyadenosine in human normal and tumour cells and tissues.
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Deoxycytidine kinase (dCK) activates several clinically important drugs, including the recently developed antileukaemic compound 2-chlorodeoxyadenosine (CdA). The distribution of dCK in cells and tissues has previously been determined by activity measurements, which may be unreliable because of the
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