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Plasma hydroxyproline and proline in primary hyperparathyroidism and in chronic uremia.
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A rapid capillary electrophoresis with electrochemiluminescence method for the assay of human urinary proline and hydroxyproline.
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A novel simultaneous determination method for free and total proline (Pro) and hydroxyproline (Hyp) in human urine was developed, based on capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection, using tris-(2,2'-bipyridyl) ruthenium(II). Experimental conditions, such as the
Simultaneous determination of proline and pipemidic acid in human urine by capillary electrophoresis with electrochemiluminescence detection.
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Pipemidic acid is extensively used in the treatment of Gram-negative urinary tract infections, and the contents of proline in human urine vary in association with chronic uremia. The simultaneous determination of pipemidic acid and proline in human urine is of significance for quality control of the
Capillary electrophoresis with electrochemiluminescence detection for simultaneous determination of proline and fleroxacin in human urine.
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The content of free proline (Pro) in body fluids is a biological parameter for patients with renal insufficiency and chronic uraemia. Fleroxacin (FLX) must be used cautiously because of adverse reactions. Its dosage must be adjusted according to the degree of renal insufficiency. In a clinical
Small intestinal dipeptidases and disaccharidases in experimental uremia in rats.
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Rats were made severely uremic with partial nephrectomy (24-hour creatinine clearance 10% of normal). Jejunal dipeptidase activities (substrates: glycyl-L-leucine, L-alanyl-L-proline, and L-methionyl-L-methionine), disaccharidase activities (maltase, sucrase, trehalase, and lactase) and morphology
Abnormalities of granulation tissue and collagen formation in experimental diabetes, uraemia and malnutrition.
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The formation of granulation tissue and collagen was studied in rats made diabetic with streptozotocin. Granulation tissue was harvested from the inside of steelmesh cylinders implanted in the back of diabetic and control animals. Four weeks after implantation there was a reduction in the quantity
Proteomics: a novel tool to unravel the patho-physiology of uraemia.
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BACKGROUND
Uraemic toxicity results in the dysfunction of many organ systems, provoking an increase in morbidity and mortality. To date, only approximately 90 uraemic retention solutes have been described. To examine unknown uraemic substances thoroughly, the identification of as many compounds as
Phosphate transport in brush border membranes from uremic rats. Response to phosphonoformic acid.
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Phosphate retention in chronic renal failure may result in the development of secondary hyperparathyroidism and renal osteodystrophy. Thus, the addition of a specific phosphaturic agent may be beneficial in the treatment of chronic renal failure. Phosphonoformic acid (PFA), a specific and
Activities of intestinal enzymes in experimental chronic renal insufficiency.
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Rats with chronic uremia following five-sixths nephrectomy showed a significant fall in the sucrase and maltase activities in the small intestinal mucosa, the lactase and cellobiase activities in contrast remained uninfluenced. The activity of the L-leucyl-L-proline and L-methionyl-L-proline
Small intestinal dipeptidases and disaccharidases in uraemic rats on a low protein diet.
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Small intestinal dipeptidases (substrate: glycyl-L-leucine, L-methionyl-L-methionine, and L-alanyl-L-proline) were determined in uraemic and sham-operated rats given normal protein diet (24%), low protein diet (6%), and total parenteral nutrition for 10-14 days. Disaccharidases (substrate: maltose,
Effect of experimental chronic renal insufficiency on bone mineral and collagen maturation.
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The effect of chronic renal disease on bone matrix and mineral maturation was evaluated in rats with experimental renal insufficiency of 2-11 wk duration utilizing bromoform-toluene gradient fractionation of bone powder, pulse labeling experiments with (45)Ca and proline-(3)H differential
In vitro human osteoblast and extracellular matrix changes after transforming growth factor beta 1 treatment.
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OBJECTIVE
Normal bone tissue is characterised by a balancing of osteoblast and osteoclast activity. The activity and differentiation of these cells are regulated by vitamins, hormones and cytokines. The action of these factors on bone tissue cells depends on the composition and mineralisation of
Protein uptake disturbs collagen homeostasis in proximal tubule-derived cells.
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BACKGROUND
Interstitial fibrosis is of major importance for the deterioration of renal function, leading to uremia. Interaction of filtered proteins with proximal tubular cells is important for the onset and development of tubulointerstitial damage.
METHODS
We investigated the effects of protein
Elimination of amino acids in chronic renal failure.
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Elimination of parenterally administered amino acids was investigated in patients with chronic renal failure (CRF) (10 on conservative treatment = CT, 13 on regular hemodialysis therapy = HD). In a bolus injection protocol 0.1 g amino acids as a 10% solution containing essential and nonessential
Differences and Effects of Metabolic Fate of Individual Amino Acid Loss in High-Efficiency Hemodialysis and Hemodiafiltration.
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