linum album/krabbamein

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Effect of aeration on production of anticancer lignans by cell suspension cultures of Linum album.

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The effects of aeration within the range of 0.2-0.5 vvm on transformed and high yielding cell cultures of Linum album were investigated in a 5-L stirred tank bioreactor equipped with low shear Setric impeller. The kinetics of cell growth, substrate utilization, and production of lignans, namely,

Potential anticancer agents II: antitumor and cytotoxic lignans from Linum album (Linaceae).

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A phytochemical study of Linum album (Linaceae), guided by bioassay with the 9KB cell culture, resulted in the isolation of podophyllotoxin and a new lignan, 3'-demethylpodophyllotoxin; alpha-and beta-peltatins were identified by comparative TLC.

In vitro polyploidy induction: changes in morphology, podophyllotoxin biosynthesis, and expression of the related genes in Linum album (Linaceae).

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UNASSIGNED Induction of tetraploidy was performed and podophyllotoxin production increased by upregulating the expression level and enzyme activity of genes related to its biosynthesis in tetraploid compared to diploid Linum album. Linum album is a valuable medicinal plant that produces antiviral

Optimization of podophyllotoxin extraction method from Linum album cell cultures.

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BACKGROUND Suspension cultures of Linum album Kotschy ex Boiss. (Linaceae) accumulate podophyllotoxin (an anticancer agent) and could therefore serve as an alternative source of this important aryltetralin lactone lignan. OBJECTIVE The present work compared four podophyllotoxin extraction methods

Time-course changes in fungal elicitor-induced lignan synthesis and expression of the relevant genes in cell cultures of Linum album.

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Linum album has been shown to accumulate anti-tumor podophyllotoxin (PTOX) and its related lignans. In the present study, we examined the effects of five fungal extracts on the production of lignans in L. album cell cultures. Fusarium graminearum extract induced the highest increase of PTOX

The effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.

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Podophyllotoxin (PTOX) is a naturally occurring phenolic compound isolated as an active anti-tumor agent. The stimulatory influence of light on the formation of phenolic compounds has been reported, but the molecular mechanism underlying the effect of light on the expression of genes involved in

Effect of in vitro morphogenesis on the production of podophyllotoxin derivatives in callus cultures of Linum album.

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The anticancer compound podophyllotoxin and other related lignans can be produced in Linum album in vitro cultures, although their biosynthesis varies according to the degree of differentiation of the plant material. In general, L. album cell cultures do not form the same lignans as roots or other

Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis.

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Treatment of Linum album cell cultures with 10 μM salicylic acid (SA) for 3 days improved podophyllotoxin (PTOX) production up to 333 μg/g dry weight (DW): over three times that of the control cultures. qPCR analyses showed that in SA-treated cells, the expression of the genes coding for

Cytotoxic Activity of Thirteen Endemic and Rare Plants from Chaharmahal and Bakhtiari Province in Iran.

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Chaharmahal and Bakhtiari Province is one of the most important endemism states of the flora of Iran with a considerable plant species diversity. In the present study, the cytotoxic activity of 13 plant species grown in Chaharmahal and Bakhtiari have been evaluated on prostate (PC-3), breast

Kinetics of glucosylated and non-glucosylated aryltetralin lignans in Linum hairy root cultures.

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Due to their pronounced cytotoxic activity, a number of aryltetralin lignans (ATLs), such as podophyllotoxin (PTOX), are used as antitumor compounds. The production of such molecules from entire plants or plant cell-tissue-organ cultures is thus of interest to the pharmaceutical industry. Hairy root

Updated biotechnological approaches developed for 2,7'-cyclolignan production1.

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2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations

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