phytate/atrophy

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Usefulness of scintigraphy with Tc-99m phytate for the diagnosis of alcoholic foamy degeneration.

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OBJECTIVE Alcoholic foamy degeneration (AFD) is a liver disease causing temporary hepatocyte dysfunction. The prognosis is usually good, but liver biopsy is needed for diagnosis. We report the usefulness of liver-spleen scintigraphy with the radiocolloid Tc-99m phytate for the diagnosis of

Effect of chitosan coatings incorporated sodium phytate on the shelf-life of Antarctic krill (Euphausia superba).

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Chitosan is a preservative with potent antibacterial activity, and sodium phytate has antioxidant activity and potent ion chelating ability. The present work aimed to explore the effects of chitosan coating combined with sodium phytate (0.1% sodium phytate + 1% chitosan) on preserving Antarctic

Dietary and nutritional biomarkers of lens degeneration, oxidative stress and micronutrient inadequacies in Indian cataract patients.

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OBJECTIVE Habitual food and nutrient intakes of 140 Indian cataract patients and 100 age- and sex-matched controls (50-75 years), from high income group and low income groups, were assessed. METHODS Food intake was recorded by food frequency questionnaire and data were examined for linkages with

Colorimetric determination of tin(II) levels in 99mTc labelling kits.

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A rapid quantitative test for determination of stannous tin in kit preparations is described. After addition of phosphomolybdate a blue colour is measured at 700 nm. Sn2+ levels in pyrophosphate, MDP, DTPA, diethyl HIDA, dimethyl HIDA, glucoheptonate, phytate and tin colloid kits were determined. In

Cellular, biological, and physicochemical basis for the hard-to-cook defect in legume seeds.

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This review integrates current knowledge on the hard-to-cook (HTC) defect in legume seeds, with emphasis on the cellular and biological changes during storage and soaking, and the physicochemical changes during heating. Several postulated mechanisms, including the pectin-cation-phytate model, cell

A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris.

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A novel phytase gene appA, with upstream and downstream sequences from Citrobacter amalonaticus CGMCC 1696, was cloned by degenerate polymerase chain reaction (PCR), and thermal asymmetric interlaced (TAIL) PCR and was overexpressed in Pichia pastoris. Sequence analysis revealed one open reading

[Antioxidants and their gastrointestinal absorption and interferences of their effects].

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Antioxidants and trace elements are using by hundreds millions of people. Effective are especially mixtures of antioxidants. Usually is declared only the composition of the tablets, but nowadays it is not satisfactory. Substantial is how much of the antioxidants is absorbed and where, how it

Phosphorus in fresh and dry dung of grazing dairy cattle, deer, and sheep: sequential fraction and phosphorus-31 nuclear magnetic resonance analyses.

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Knowledge of phosphorus (P) fractions in dung of animals (dairy cattle, deer, sheep) grazing pasture is important for soil fertility and the potential for P transport in runoff and subsequent surface water quality deterioration. We used sequential fractionation and 31P nuclear magnetic resonance

Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger.

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Phytase catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. A gene (phyA) of Aspergillus niger NRRL3135 coding for extracellular, glycosylated phytase was isolated using degenerate oligodeoxyribonucleotides deduced from phytase amino acid (aa)

Cloning, expression, and characterization of a new phytase from the phytopathogenic bacterium Pectobacterium wasabiae DSMZ 18074.

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The soft rot bacterium Pectobacterium wasabiae is an economically important pathogen of many crops. A new phytase gene, appA, was cloned from P. wasabiae by degenerate PCR and TAIL-PCR. The open reading frame of appA consisted of 1,302 bp encoding 433 amino acid residues, including 27 residues of a

A novel phytase with preferable characteristics from Yersinia intermedia.

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A Yersinia intermedia strain producing phytase was isolated from glacier soil. The phytase gene, appA, was isolated by degenerate PCR and TAIL-PCR. The full-length fragment contained 2354bp with a 1326-bp open reading frame encoding 441 amino acids. APPA contained the active site RHGXRXP and HD

Gene cloning, expression, and characterization of a novel phytase from Dickeya paradisiaca.

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A novel phytase gene, appA, was isolated by degenerate polymerase chain reaction (PCR) and thermal asymmetric interlaced PCR from Dickeya paradisiaca. The full-length appA comprises 1278 bp and encodes 425 amino acid residues, including a 23-residue putative N-terminal signal peptide. The deduced

A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive.

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A phytase with high activity at neutral pH and typical water temperatures ( approximately 25 degrees C) could effectively hydrolyze phytate in aquaculture. In this study, a phytase-producing strain, Pedobacter nyackensis MJ11 CGMCC 2503, was isolated from glacier soil, and the relevant gene, PhyP,

Purification, sequencing and evaluation of a divergent phytase from Penicillium oxalicum KCTC6440.

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A fungal strain producing high levels of phytase was purified to homogeneity from Penicillium oxalicum KCTC6440 (PhyA). The molecular mass of the purified PhyA was 65 kDa and optimal activity occurred at 55°C. The enzyme was stable in a pH range of 4.5-6.5, with an optimum performance at pH 5.5. The

Impact of the discovery of human zinc deficiency on health.

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The essentiality of zinc was recognized 46 years ago. Zinc deficiency resulting in growth retardation, hypogonadism, immune dysfunction and cognitive impairment affects nearly 2 billion subjects in the developing world. High phytate content of the cereal proteins consumed in the developing world,

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