[Studies on callus growth and phillyrin accumulation of Forsythia suspensa].

OBJECTIVE

To investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa.

METHODS

The cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents.

CONCLUSIONS

Growth cycle of cells is twenty-eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth. The optimum medium is MS. The optimum hormones concentrations are 1 mg.L-1 2,4-D, 0.5 mg.L-1 6-BA and 0.5 mg.L-1KT. The cell culture in light is more suitable than that in dark.