How does Malus crabapple resist ozone? Transcriptomics and metabolomics analyses

Ozone (O₃), an oxidizing toxic air pollutant, is ubiquitous in industrialized and developing countries. To understand the effects of O₃ exposure on apple (Malus) and to explore its defense mechanisms, we exposed 'Hongjiu' crabapple to O₃ and monitored its responses using physiological, transcriptomics, and metabolomics analyses. Exposure to 300 nL L^-1 O₃ for 3 h caused obvious damage to the leaves of Malus crabapple, affected chlorophyll and anthocyanin contents, and activated antioxidant enzymes. The gene encoding phospholipase A was highly responsive to O₃ in Malus crabapple. McWRKY75 is a key transcription factor in the response to O₃ stress, and its transcript levels were positively correlated with those of flavonoid-related structural genes (McC4H, McDFR, and McANR). The ethylene response factors McERF019 and McERF109-like were also up-regulated by O₃. Exogenous methyl jasmonate (MeJA) decreased the damaging effects of O₃ on crabapple and was most effective at 200 μmol L ^-1. Treatments with MeJA altered the metabolic pathways of crabapple under O₃ stress. In particular, MeJA activated the flavonoid metabolic pathway in Malus, which improved its resistance to O₃ stress.

Keywords: Metabolomics; Methyl jasmonate; Molecular mechanism; Ozone; Physiological mechanism; Transcriptomics.