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14 risultati
Rapid detection and isolation of known and putative alpha-L-arabinofuranosidase genes using degenerate PCR primers.
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alpha-L-Arabinofuranosidases (AFases; EC 3.2.1.55) are exo-type enzymes, which hydrolyze terminal nonreducing arabinose residues from various polysaccharides such as arabinan and arabinoxylan. Genome-wide BLAST search showed that various bacterial strains possess the putative AFase genes with
Exocellular proteases of Malbranchea gypsea and their role in keratin deterioration.
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Malbranchea gypsea IMI 338,168 isolated from the soils of Keoladeo National Park, Bharatpur was studied for its ability to produce exocellular proteases on glucose-gelatin medium at pH 7; 28 degrees C. The fungus was observed to be a potent producer of such enzymes. Protease production was optimal
Use of xylanase and arabinofuranosidase for arabinose removal from unbleached kraft pulp.
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Preparations of arabinofuranosidase and xylanase, respectively from Aureobasidium pullulans and Trichoderma longibrachiatum, were used to remove selectively xylose and arabinose from kraft pulp. The equilibrium moisture content of pulps treated with both enzymes, at varying relative humidities,
Redistribution and degeneration of retinal astrocytes in experimental murine cerebral malaria: relationship to disruption of the blood-retinal barrier.
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To determine whether astrocytes play a critical role in the pathogenesis of experimental murine cerebral malaria (EMCM), we examined changes in astrocyte morphology and distribution, using retinal wholemounts, in three models: a fatal cerebral malaria (CM) model, in which mice die showing cerebral
Separation of furans and carboxylic acids from sugars in dilute acid rice straw hydrolyzates by nanofiltration.
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This work studied the concentration of hydrolyzates obtained from dilute acid hydrolysis of rice straw using nanofiltration (NF). In order to minimize the Donnan exclusion effect of the membrane, the hydrolyzate solution was controlled at low pH value. Negative retentions of both furans and
[The 72nd Annual Meeting Education Lecture. Cord factor].
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Mycobacterial cell wall contains various lipids (glycolipids and phospholipids) to contribute to its hydrophobic property or acid-fastness and these surface molecules contact with host cells in the early step of infection. Among them, cord factor (trehalose 6,6'-dimycolate, TDM or CF) is the most
Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening.
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Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and
Functional analysis of arabinofuranosidases and a xylanase of Corynebacterium alkanolyticum for arabinoxylan utilization in Corynebacterium glutamicum.
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Xylooligosaccharides (XOSs) and arabinoxylooligosaccharides (AXOSs) are major oligosaccharides derived from arabinoxylan. In our previous report, Corynebacterium glutamicum was engineered to utilize XOSs by introducing Corynebacterium alkanolyticum xyloside transporter and β-xylosidase. However,
alpha-l-Arabinofuranosidase from strawberry fruit: cloning of three cDNAs, characterization of their expression and analysis of enzymatic activity in cultivars with contrasting firmness.
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Softening of fleshy fruits during ripening is associated to catabolism of cell wall components. In strawberry, pectin degradation, as well as loss of neutral sugars (mainly arabinose), increases during ripening, and probably contributes to fruit softening. In this work, we report the activity of
Cellular mechanisms of the blood-brain barrier (BBB) opening to albumin-gold complex.
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Cold lesion injury applied to mouse brain and infusion of hyperosmolar L(+) arabinose solution into rat carotid artery were used as extravascular and intravascular insults, respectively, leading to blood-brain barrier (BBB) disruption. To study the cellular mechanisms of the BBB opening,
THE INFLUENCE OF CARBOHYDRATES ON THE CULTIVATION OF SPIROCHETES.
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Various carbohydrates have been added to the fluid cultures of different strains of spirochetes in order to determine the behavior of the latter toward the carbohydrates. In the present experiment, amygdalin, arabinose, beerwort, dextrin, galactose, glycogen, glucose, inulin, lactose, levulose,
The lethal principle of Poa huecu (coiron blanco): a plant indigenous to Argentina.
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The lethality of Poa huecu, a plant toxic to cattle and sheep, was followed by injection of chromatographic fractions in mice. The lethal aqueous extract was administered i.p. to Rockland mice of either sex and produced motor incoordination, transient ataxia, rough hair coat, tremors and muscle
Scavenging of free radicals, antimicrobial, and cytotoxic activities of the Maillard reaction products of beta-lactoglobulin glycated with several sugars.
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The Maillard reaction occurs during many industrial and domestic thermal treatments of foods. It is widely used because of its role in creating colors, flavors, textures, and other functional properties in foodstuffs. Proteins glycated without the use of conventional chemical reagents have improved
Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease
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Nonalcoholic fatty liver disease (NAFLD) is one of the prevalent and typical chronic liver diseases. In this study, we extracted a novel Angelica sinensis polysaccharide (ASP) with low molecular weight (MW) of 3.2 kDa through optimized "one-step" purification process. The major monosaccharide
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