asthenozoospermia/tyrosine

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Nitric oxide synthase and tyrosine nitration in idiopathic asthenozoospermia: an immunohistochemical study.

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OBJECTIVE To characterize the expression pattern of constitutive and inducible nitric oxide synthase (NOS) in spermatozoa isolated from normospermic fertile donors and asthenozoospermic infertile patients, by means of immunohistochemistry. Also to evaluate the immunohistochemical expression of

[Identification of asthenozoospermia-associated proteins in human seminal plasma by shotgun proteomic strategy].

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OBJECTIVE To identify asthenozoospermia-associated proteins in seminal plasma by the shotgun proteomic strategy. METHODS Six seminal plasma samples were collected by Percoll respectively from healthy fertile and asthenozoospermia volunteers, balanced, mixed, and then the mixture was separated by

Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples.

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Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of

Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.

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To characterize in depth and investigate the role of exosomes present in seminal plasma in affecting parameters underlying sperm activity.In vitro experimental study.Research

Molecular study of human sperm RNA: Ropporin and CABYR in asthenozoospermia.

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BACKGROUND Sperm motility is an essential aspect of human fertility. Sperm contain an abundance of transcripts, thought to be remnants of mRNA, which comprise a genetic fingerprint and can be considered a historic record of gene expression during spermatogenesis. The aberrant expression of numerous

EMC10 governs male fertility via maintaining sperm ion balance.

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Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples, and male infertility accounts for half of total infertility. Studies on genetically modified animal models have identified lots of genes involved in the pathogenesis of male infertility. The

Membrane-bound Glucose regulated protein 78 interacts with alpha-2-macroglobulin to promote actin reorganization in sperm during epididymal maturation.

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Glucose regulated protein 78 (GRP78) is expressed on cell surface in exceptional conditions as seen in cancer cells and macrophages. We have reported its membrane localization in sperm. The functional significance of its surface localization in sperm is an enigma. Alpha-2-macroglobulin (α2M)

Motility enhancement of human spermatozoa using electrical stimulation in the nano-Ampere range with enzymatic biofuel cells.

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Sperm motility is a crucial factor for normal fertilisation that is partly supported by mitochondrial activity. Enzymatic biofuel cells (EBFCs) generate electric currents by an electron grade from anodic to cathodic electrodes in a culture media. We demonstrate that electrical stimulation by EBFC at

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy.

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OBJECTIVE The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. METHODS A total of 103

Functions of TAM RTKs in regulating spermatogenesis and male fertility in mice.

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Mice lacking TYRO3, AXL and MER (TAM) receptor tyrosine kinases (RTKs) are male sterile. The mechanism of TAM RTKs in regulating male fertility remains unknown. In this study, we analyzed in more detail the testicular phenotype of TAM triple mutant (TAM(-/-)) mice with an effort to understand the

The production of peroxynitrite by human spermatozoa may affect sperm motility through the formation of protein nitrotyrosine.

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OBJECTIVE To detect peroxynitrite and 3-nitrotyrosine production in human spermatozoa of asthenozoospermic infertile patients and normospermic donors, and evaluate any influence on kinetic sperm features. METHODS Basic study. METHODS Andrology Unit, Dept of Internal Medicine and Biochemistry

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