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choline acetyltransferase/sarcoma
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[Immunohistochemical analysis of tumor cells using choline acetyltransferase (author's transl)].
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For an immunohistochemical analysis of cellular function of tumors, as related to acetylcholine, the antibody to choline acetyltransferase from bovine brain was obtained in guinea pigs. The specificity of the antibody was immunohistochemically studied in the cervical spinal cord of the mouse. And
Clinical application of intraoperative measurement of choline acetyltransferase activity during functioning free muscle transfer.
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Intraoperative measurement of choline acetyltransferase (CAT) activity was used for evaluation of the functional status of donor nerves during functioning free muscle transfer (FFMT). Twelve patients underwent the procedure. Seven patients had a brachial plexus injury, 3 Volkmann's contracture, 1
Indistinguishable patterns of protooncogene expression in two distinct but closely related tumors: Ewing's sarcoma and neuroepithelioma.
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Genetic characterization of human tumors promises new insights of biological importance and clinical relevance. We have found that two solid tumors, peripheral neuroepithelioma and Ewing's sarcoma of bone, which share a common cytogenetic rearrangement, are characterized by an indistinguishable and
A Ewing's sarcoma cell line showing some, but not all, of the traits of a cholinergic neuron.
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The Ewing's sarcoma cell line ICB 112 was examined in detail for a cholinergic phenotype. Choline acetyltransferase activity (12.3 +/- 2.9 nmol/h/mg of protein) was associated with the presence of multiple mRNA species labeled with a human choline acetyltransferase riboprobe. Choline was taken up by
Modulation of retinal differentiation by oncogenes: effect of the v-src gene on expression of choline acetyltransferase and glutamine synthetase.
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Expression of the protooncogene c-src in chick neural retina is developmentally regulated and associated with neural differentiation. In the present study, chick neural retina (NR) cell cultures from 7 day embryos were exposed to the exogenous src oncogene, the c-src counterpart, to establish the
Expression of neuronal markers in chick and quail embryo neuroretina cultures infected with Rous sarcoma virus.
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Cultures of neuroretina (NR) cells from 7-day chick and quail embryos were infected with ts NY-68, a thermosensitive mutant of Rous sarcoma virus (RSV) which transformed NR cells at 36 degrees C. The following differentiation markers for neurones were studied: tetanus toxin-binding sites at the cell
A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture.
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Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors, glial (Müller) cells and horizontal, bipolar, amacrine and ganglion neuronal cells. We describe here the usefulness of Rous sarcoma virus (RSV) in the establishment of a neuronal clone from
Altered cellular functions in a PC-12 cell clone chronically infected with retrovirus.
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We characterized retrovirus-induced changes in PC-12 cell function and neuronal differentiation. PC-12 cells were infected with a neurotropic retrovirus (temperature-sensitive Moloney murine leukemia virus, mutant BA-1). We isolated a cell clone from this infected culture that displayed altered
Cooperation between phosphorylation and acetylation processes in transcriptional control.
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We previously reported that the activation of the M promoter of the human choline acetyltransferase (ChAT) gene by butyrate and trapoxin in transfected CHP126 cells is blocked by PD98059, a specific mitogen-activated protein kinase kinase (MEK) inhibitor (E. Espinos and M. J. Weber, Mol. Brain Res.
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