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daphnoretin/stellera
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7 risultati
Orthogonal test design for optimization of supercritical fluid extraction of daphnoretin, 7-methoxy-daphnoretin and 1,5-diphenyl-1-pentanone from Stellera chamaejasme L. and subsequent isolation by high-speed counter-current chromatography.
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Supercritical fluid extraction (SFE) of daphnoretin, 7-methoxy-daphnoretin and 1,5-diphenyl-1- pentanone from Stellera chamaejasme L. was performed. An orthogonal L9 (3)4 test design was applied to select the optimum extraction parameters including pressure, temperature, modifier and sample particle
Development of a method to screen and isolate bioactive constituents from Stellera chamaejasme by ultrafiltration-liquid chromatography combined with semi-preparative high-performance liquid chromatography and high-speed counter-current chromatography.
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A simple and efficient method based on ultrafiltration-liquid chromatography-mass spectrometry was used for the rapid screening and identification of ligands in the extracts of Stellera chamaejasme. The bound ligands, i.e., daphnoretin, isopimpinellin, chamaechromone, neochamaejasmin A, and
Efficient combination of circulating ultrasound-assisted extraction and centrifugal partition chromatography for extraction and on-line separation of chemical constituents from Stellera chamaejasme L.
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BACKGROUND
Sample preparation is a crucial step in medicinal herb analysis because the desired chemical components need to be extracted from the herbal materials for further separation and characterisation. Thus, the development of " modern" sample preparation techniques with significant advantages
Phytotoxicity of umbelliferone and its analogs: Structure-activity relationships and action mechanisms.
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Two coumarins, umbelliferone and daphnoretin, were isolated from roots of Stellera chamaejasme L; the former had been identified as one of the main allelochemicals in our previous studies. Both of them have the skeleton of 7-hydroxycoumarin, but showed different phytotoxic effects. Umbelliferone and
Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract.
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Isochamaejasmin, neochamaejasmin A and daphnoretin derived from Stellera chamaejasme L. are important because of their reported anticancer properties. In this study, a sensitive UPLC-MS/MS method for the determination of isochamaejasmin, neochamaejasmin A and daphnoretin in rat plasma was developed.
[Studies on chemical constituents of Stellera chamejasma L].
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OBJECTIVE
Isolate and identify the bioactive compounds from the root of Stellera chamejasma.
METHODS
The compounds were extracted with solvents, isolated by column chromatography and identified by spectroscopic methods.
RESULTS
Seven compounds were isolated and identified as umbelliferone(1);
7-Hydroxy-6-methoxy-3-[(2-oxo-2H-1-benzopyran-7-yl)oxy]-2H-1-benzopyran-2-one.
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The title compound, daphnoretin, C19H12O7, was isolated from the leaves of Stellera chamaejasme L. Two independent molecules are present in the asymmetric unit, with similar conformations. Each of the independent molecules is composed of two chromene systems connected by an ether bridge. The
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