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Pagina 1 a partire dal 2840 risultati
[Proline-dependent wild strains and proline-dependent mutants of the plague microbe].
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It is found that the growth of Yersinia pestis wild strains, isolated from Citellus musicus Menetrié in the Central Caucasus, depends on the presence of proline in the medium. Proline can not be substituted by glutamic acid, other amino acids or vitamins. 28 proline-requiring mutants were selected
[Evaluation of cold resistance of four wild Carex speices].
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Four wild Carex speices (C. rigescens, C. lancifolia, C. leucochlora and C. humilis) collected from Mount Taishan were used as experimental materials. The adaption across the winter and physiological characteristics resistant to cold stress were investigated, semi-lethal temperature (LT50) was
Characterization of drought resistance in a wild relative of wheat, Triticum kotschyi.
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Wild relatives of wheat have served as a genetic source for economically useful traits. A better understanding of the mechanisms underlying such traits may be useful in the genetic transfer and selection processes. Research was undertaken to compare the effects of controlled water stress on
Salt-tolerance and proline accumulation: a comparative study in salt-tolerant and wild type cultured cells of eggplant.
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A NaCl-tolerant cell line of eggplant has been isolated, as a spontaneous variant, on agar solidified medium supplemented with 1% NaCl (Electrical conductivity -17.5 m mho/cm), a concentration lethal to the wild type cells. The stability of the altered response of the selected clone was confirmed by
Ligand-induced conformational changes in wild-type and mutant yeast pyruvate kinase.
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A mutant form of pyruvate kinase in which serine 384 has been mutated to proline has been engineered in the yeast Saccharomyces cerevisiae. Residue 384 is located in a helix in a subunit interface of the tetrameric enzyme, and the mutation was anticipated to alter the conformation of the helix and
Comparative study on free amino acid composition of wild edible mushroom species.
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A comparative study on the amino acid composition of 11 wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, and
The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli.
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Positive effects of proline addition on the central metabolism of wild-type and lactic acid-producing Saccharomyces cerevisiae strains.
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In Saccharomyces cerevisiae, proline is a stress protectant interacting with other substrate uptake systems against oxidative stress under low pH conditions. In this study, we performed metabolomics analysis to investigate the response associated with an increase in cell growth rates and maximum
Molecular characterization of of alpha-gliadin genes from wild emmer wheat (Triticum dicoccoides).
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According to the two distal and conserved regions of known alpha-gliadin genes, gene-specific primers for alpha-gliadin were designed, and the coding regions of four gliadin genes (i.e. GliTd-1, GliTd-2, GliTd-3 and GliTd-4) with the length of about 800 bp were isolated from the genomic DNA of wild
Taste responses to sweet stimuli in alpha-gustducin knockout and wild-type mice.
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The importance of alpha-gustducin in sweet taste transduction is based on data obtained with sucrose and the artificial sweetener SC45647. Here we studied the role of alpha-gustducin in sweet taste. We compared the behavioral and electrophysiological responses of alpha-gustducin knockout (KO) and
Functional and structural studies of wild type SOX9 and mutations causing campomelic dysplasia.
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In humans, mutations in SOX9 result in a skeletal malformation syndrome, campomelic dysplasia (CD). The present study investigated two major classes of CD mutations: 1) point mutations in the high mobility group (HMG) domain and 2) truncations and frameshifts that alter the C terminus of the
Biosynthesis of prodigiosin by non-proliferating wild-type Serratia marcescens and mutants deficient in catabolism of alanine, histidine, and proline.
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Mutants of Serratia marcescens Nima, designated as Aut, Hut, or Put, did not utilize L-alanine, L-histidine, or L-proline, respectively, as a sole carbon source but did utilize other amino acids or glycerol as carbon sources. The bacteria were permeable to alanine, histidine, and proline but lacked
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.
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The genes proA and proB encoding the first two enzymes of the proline biosynthetic sequence in Escherichia coli were subcloned from a ColE1 hybrid plasmid containing 23.3 kilobases of genomic DNA. proA and proB are contiguous and constitute a single operon transcribed in the direction proB-proA. The
Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins.
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BACKGROUND
The mitogen-activated protein kinases (MAPKs) pathway is critical for cellular signaling, and proteins such as phosphatases that regulate this pathway are important for normal tissue development. Based on our previous work on dual specificity phosphatase-5 (DUSP5), and its role in
Polymorphism in wild-type p53 modulates response to chemotherapy in vitro and in vivo.
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A single-nucleotide polymorphism (SNP) in exon 4 results in expression of either arginine (72R) or proline (72P) at codon 72 of p53. We demonstrate that the in vitro response of cells exposed to anticancer agents is strongly influenced by this SNP in wild-type p53. In inducible systems and in cells
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