The sesquiterpene lactone eupatoriopicrin (EUP) from Eupatorium cannabinum L. has been shown to be cytotoxic in a glutathione (GSH)-dependent way. In order to assess possible DNA damage as a cause for cell death, the study reported was initiated. After 2 hr incubation of Ehrlich ascites tumour cells
The activity was determined in tissue culture of KB, HeLa human tumor cells. RK normal animal and EAC-E4 Ehrlich ascites carcinoma cells. Eupatolide (EUP) and eupatoriopicrin (EUPP) show similar cytotoxic activity (ED50 from 0-5 to 1-3 mug/ml), dependent on the cell strain used. In plate test EUP