lupinus digitatus/protease

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Effects of the aspartic protease inhibitor from Lupinus bogotensis seeds on the growth and development of Hypothenemus hampei: an inhibitor showing high homology with storage proteins.

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התחבר הרשם

The coffee berry borer Hypothenemus hampei is a pest that causes great economic damage to coffee grains worldwide. Because the proteins consumed are digested by aspartic proteases in the insect's midgut, the inhibition of these proteases by transferring a gene encoding an aspartic protease inhibitor

An inhibitor from Lupinus bogotensis seeds effective against aspartic proteases from Hypothenemus hampei.

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התחבר הרשם

The coffee berry borer, Hypothenemus hampei (Ferrari), is one of the most devastating coffee pests (Coffea arabica L.) worldwide. Digestion in the midgut of H. hampei is facilitated by aspartic proteases. This is the first report of an aspartic protease inhibitor from Lupinus bogotensis. The L.

[Activities of protein-inhibitors of proteases in seeds of different varieties of yellow and white lupine plants].

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התחבר הרשם

Activities of protein-inhibitors of proteases and their correlation with the total content of protein and alkaloids in yellow and white lupine seeds were studied. Yellow lupine seeds showed lowe activities of protein-inhibitors. They also displayed negative correlation between activities of

Purification and properties of a protease from Lupinus angustifolius during germination.

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התחבר הרשם

A protease, present in Lupinus angustifolius cotyledons on the fifth day after germination and assayable by following the release of amino groups from gliadin has been purified to the degree that the associated protein of the extract is undetectable. The enzyme is capable, under varying conditions,

The effect of protease and Bacillus licheniformis on nutritional value of pea, faba bean, yellow lupin and narrow-leaved lupin in broiler chicken diets.

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התחבר הרשם

This study investigated the nutritional value (digestibility of ileal crude protein and amino acids (AAs)) and the AME_N value of legumes (pea, faba bean, yellow lupin, and narrow-leaved lupin) with or without protease.Two hundred, one-day-old, male Ross 308 broiler chickens were randomly

Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.

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התחבר הרשם

Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry

Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds.

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התחבר הרשם

The paper describes the purification, structural characterization and inhibitory properties of a trypsin inhibitor from Lupinus albus L., a leguminous plant believed to be devoid of any protease inhibitor. The protein has been isolated by a newly set-up procedure and characterized by direct amino

Effect of enzyme supplementation on the nutritional value of raw, autoclaved, and dehulled lupins (Lupinus albus) in chicken diets.

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התחבר הרשם

The effects of adding crude enzyme preparations to diets containing raw, autoclaved, and dehulled lupin seeds on the performance of broiler and Leghorn chicks (7 to 21 days) were evaluated in five experiments. In the first experiment, enzyme addition (combination of Energex-carbohydrase, Bio-Feed

Structure of γ-conglutin: insight into the quaternary structure of 7S basic globulins from legumes.

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התחבר הרשם

γ-Conglutin from lupin seeds is an unusual 7S basic globulin protein. It is capable of reducing glycaemia in mammals, but the structural basis of this activity is not known. γ-Conglutin shares a high level of structural homology with glycoside hydrolase inhibitor proteins, although it lacks any kind

[Artifactual multiple forms of beta-cyanoalanine synthase from white lupine].

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התחבר הרשם

The three active forms of beta-cyanoalanine synthase (EC 4.4.1.9) from white lupine seedlings were obtained in a homogeneous state and some physico-chemical and catalytic properties of the enzyme, i.e. isoelectric points, molecular weight, amino acid composition, Km, substrate and cosubstrate

Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights.

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התחבר הרשם

Feeding trials have been done with rats to assess the effects of long-term (700 d) consumption of diets based on raw cowpeas (Vigna unguiculata; moderate Bowman-Birk inhibitor content, low lectin content), lupin seeds (Lupinus angustifolius; low lectin and protease inhibitor content) or soya beans

Effects of feeding growing broiler chickens with practical diets containing sweet lupin (Lupinus angustifolius) seed meal.

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התחבר הרשם

1. Two experiments with growing broiler chickens were carried out to study the effects of the inclusion in their diets of whole or dehulled lupin (Lupinus angustifolius) seed meal on productive and physiological parameters. The effects of the addition of a commercial protease (1 g/kg, Bio-Feed Pro,

Some 2S albumin from peanut seeds exhibits inhibitory activity against Aspergillus flavus.

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התחבר הרשם

A crude 2S albumin fraction was separated from peanut (Arachis hypogaea L.) cotyledons. Untreated 2S albumin had little inhibitory activity against trypsin, spore germination, or hyphal growth of Aspergillus flavus. However, following treatment of 2S albumin with SDS, increased inhibitory activity

Effect of water stress on lupin stem protein analysed by two-dimensional gel electrophoresis.

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התחבר הרשם

Lupinus albus plants can withstand severe drought stress and show signs of recovery 24 h after rewatering (RW). Two-dimensional gel electrophoresis was used to evaluate the effect of water deficit (WD) on the protein composition of the two components of the lupin stem (stele and cortex). This was

Molecular characterization of a novel quinolizidine alkaloid O-tigloyltransferase: cDNA cloning, catalytic activity of recombinant protein and expression analysis in Lupinus plants.

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התחבר הרשם

A novel acyltransferase committed to the final step of quinolizidine alkaloid biosynthesis, tigloyl-CoA:(-)-13alpha-hydroxymultiflorine/(+)-13alpha-hydroxylupanine O-tigloyltransferase, has been purified from Lupinus albus. The internal amino acid sequences were determined with protease-digested

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Herbal & Natural Medicine

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